No Cover Image

Journal article 88 views

A validated reverse-phase high performance liquid chromatography (RP-HPLC) method for the quantification of Gamma- tocotrienol in tocotrienol rich fractions of crude palm oil

Rajesh Sreedharan Nair, Nashiru Billa, Andrew Morris Orcid Logo

Current Nutrition & Food Science, Volume: 17

Swansea University Author: Andrew Morris Orcid Logo

Full text not available from this repository: check for access using links below.

Abstract

Background: Palm oil and its constituents have wide applications in food, cosmetics and pharmaceutical industries. The tocotrienol-rich fractions of crude palm oil have drawn greater research interest in recent years due to its potent health benefits. Therefore, reliable and validated analytical met...

Full description

Published in: Current Nutrition & Food Science
ISSN: 1573-4013
Published: Bentham Science Publishers Ltd. 2021
Online Access: Check full text

URI: https://cronfa.swan.ac.uk/Record/cronfa57832
Tags: Add Tag
No Tags, Be the first to tag this record!
first_indexed 2021-09-09T21:39:16Z
last_indexed 2021-11-13T04:24:21Z
id cronfa57832
recordtype SURis
fullrecord <?xml version="1.0"?><rfc1807><datestamp>2021-11-12T16:24:35.5198814</datestamp><bib-version>v2</bib-version><id>57832</id><entry>2021-09-09</entry><title>A validated reverse-phase high performance liquid chromatography (RP-HPLC) method for the quantification of Gamma- tocotrienol in tocotrienol rich fractions of crude palm oil</title><swanseaauthors><author><sid>3d7a7f540a5143114fcaf67e4c68d151</sid><ORCID>0000-0002-6315-7553</ORCID><firstname>Andrew</firstname><surname>Morris</surname><name>Andrew Morris</name><active>true</active><ethesisStudent>false</ethesisStudent></author></swanseaauthors><date>2021-09-09</date><deptcode>PHAR</deptcode><abstract>Background: Palm oil and its constituents have wide applications in food, cosmetics and pharmaceutical industries. The tocotrienol-rich fractions of crude palm oil have drawn greater research interest in recent years due to its potent health benefits. Therefore, reliable and validated analytical methods are essential for the quantification of tocotrienols.Objective: This study aimed to develop a simple and economical RP-HPLC method for the quantification of gamma-tocotrienol in tocotrienol rich fractions of crude palm oil.Methods: An Agilent HPLC system supplied with a Diode Array detector and an auto-injector system was used for the method development, and the wavelength was set 295 nm. A reversed-phase C18 column maintained at 30 &#xB0;C using a mobile phase composition of methanol: water (95:05) at a flow rate of 1 mL/min was used for the analysis. The developed method was validated according to ICH guidelines.Results and discussion: A symmetrical peak of gamma-tocotrienol was observed at 8.7 minutes with minimal peak tailing (between 0.76 and 0.78), and an acceptable resolution above 2.0. Excellent linearity was evident with R2 values 0.9996 and 0.9991 for intra-day and inter-day respectively. The method demonstrated a high precision (%RSD values &#x2264; 5.8%) and accuracy (%RE&lt;9.6%). The LOD and LOQ of gamma-tocotrienol were determined as 1.4 &#x3BC;g/mL and 4.2 &#x3BC;g/mL respectively. The system suitability studies indicate that the chromatographic parameters are well within the acceptable limit.Conclusion: In conclusion, the developed RP-HPLC method is rapid, precise, stable and economical for the quantification of gamma-tocotrienol.</abstract><type>Journal Article</type><journal>Current Nutrition &amp; Food Science</journal><volume>17</volume><journalNumber/><paginationStart/><paginationEnd/><publisher>Bentham Science Publishers Ltd.</publisher><placeOfPublication/><isbnPrint/><isbnElectronic/><issnPrint>1573-4013</issnPrint><issnElectronic/><keywords>TRF, gamma-tocotrienol, RP-HPLC, validation, ICH, system suitability.</keywords><publishedDay>3</publishedDay><publishedMonth>8</publishedMonth><publishedYear>2021</publishedYear><publishedDate>2021-08-03</publishedDate><doi>10.2174/1573401317666210803155717</doi><url/><notes/><college>COLLEGE NANME</college><department>Pharmacy</department><CollegeCode>COLLEGE CODE</CollegeCode><DepartmentCode>PHAR</DepartmentCode><institution>Swansea University</institution><apcterm/><lastEdited>2021-11-12T16:24:35.5198814</lastEdited><Created>2021-09-09T22:35:10.6206819</Created><path><level id="1">Swansea University Medical School</level><level id="2">Swansea University Medical School</level></path><authors><author><firstname>Rajesh Sreedharan</firstname><surname>Nair</surname><order>1</order></author><author><firstname>Nashiru</firstname><surname>Billa</surname><order>2</order></author><author><firstname>Andrew</firstname><surname>Morris</surname><orcid>0000-0002-6315-7553</orcid><order>3</order></author></authors><documents><document><filename>Under embargo</filename><originalFilename>Under embargo</originalFilename><uploaded>2021-09-13T17:13:39.3246276</uploaded><type>Output</type><contentLength>530072</contentLength><contentType>application/pdf</contentType><version>Accepted Manuscript</version><cronfaStatus>true</cronfaStatus><embargoDate>2022-08-03T00:00:00.0000000</embargoDate><copyrightCorrect>true</copyrightCorrect><language>eng</language></document></documents><OutputDurs/></rfc1807>
spelling 2021-11-12T16:24:35.5198814 v2 57832 2021-09-09 A validated reverse-phase high performance liquid chromatography (RP-HPLC) method for the quantification of Gamma- tocotrienol in tocotrienol rich fractions of crude palm oil 3d7a7f540a5143114fcaf67e4c68d151 0000-0002-6315-7553 Andrew Morris Andrew Morris true false 2021-09-09 PHAR Background: Palm oil and its constituents have wide applications in food, cosmetics and pharmaceutical industries. The tocotrienol-rich fractions of crude palm oil have drawn greater research interest in recent years due to its potent health benefits. Therefore, reliable and validated analytical methods are essential for the quantification of tocotrienols.Objective: This study aimed to develop a simple and economical RP-HPLC method for the quantification of gamma-tocotrienol in tocotrienol rich fractions of crude palm oil.Methods: An Agilent HPLC system supplied with a Diode Array detector and an auto-injector system was used for the method development, and the wavelength was set 295 nm. A reversed-phase C18 column maintained at 30 °C using a mobile phase composition of methanol: water (95:05) at a flow rate of 1 mL/min was used for the analysis. The developed method was validated according to ICH guidelines.Results and discussion: A symmetrical peak of gamma-tocotrienol was observed at 8.7 minutes with minimal peak tailing (between 0.76 and 0.78), and an acceptable resolution above 2.0. Excellent linearity was evident with R2 values 0.9996 and 0.9991 for intra-day and inter-day respectively. The method demonstrated a high precision (%RSD values ≤ 5.8%) and accuracy (%RE<9.6%). The LOD and LOQ of gamma-tocotrienol were determined as 1.4 μg/mL and 4.2 μg/mL respectively. The system suitability studies indicate that the chromatographic parameters are well within the acceptable limit.Conclusion: In conclusion, the developed RP-HPLC method is rapid, precise, stable and economical for the quantification of gamma-tocotrienol. Journal Article Current Nutrition & Food Science 17 Bentham Science Publishers Ltd. 1573-4013 TRF, gamma-tocotrienol, RP-HPLC, validation, ICH, system suitability. 3 8 2021 2021-08-03 10.2174/1573401317666210803155717 COLLEGE NANME Pharmacy COLLEGE CODE PHAR Swansea University 2021-11-12T16:24:35.5198814 2021-09-09T22:35:10.6206819 Swansea University Medical School Swansea University Medical School Rajesh Sreedharan Nair 1 Nashiru Billa 2 Andrew Morris 0000-0002-6315-7553 3 Under embargo Under embargo 2021-09-13T17:13:39.3246276 Output 530072 application/pdf Accepted Manuscript true 2022-08-03T00:00:00.0000000 true eng
title A validated reverse-phase high performance liquid chromatography (RP-HPLC) method for the quantification of Gamma- tocotrienol in tocotrienol rich fractions of crude palm oil
spellingShingle A validated reverse-phase high performance liquid chromatography (RP-HPLC) method for the quantification of Gamma- tocotrienol in tocotrienol rich fractions of crude palm oil
Andrew Morris
title_short A validated reverse-phase high performance liquid chromatography (RP-HPLC) method for the quantification of Gamma- tocotrienol in tocotrienol rich fractions of crude palm oil
title_full A validated reverse-phase high performance liquid chromatography (RP-HPLC) method for the quantification of Gamma- tocotrienol in tocotrienol rich fractions of crude palm oil
title_fullStr A validated reverse-phase high performance liquid chromatography (RP-HPLC) method for the quantification of Gamma- tocotrienol in tocotrienol rich fractions of crude palm oil
title_full_unstemmed A validated reverse-phase high performance liquid chromatography (RP-HPLC) method for the quantification of Gamma- tocotrienol in tocotrienol rich fractions of crude palm oil
title_sort A validated reverse-phase high performance liquid chromatography (RP-HPLC) method for the quantification of Gamma- tocotrienol in tocotrienol rich fractions of crude palm oil
author_id_str_mv 3d7a7f540a5143114fcaf67e4c68d151
author_id_fullname_str_mv 3d7a7f540a5143114fcaf67e4c68d151_***_Andrew Morris
author Andrew Morris
author2 Rajesh Sreedharan Nair
Nashiru Billa
Andrew Morris
format Journal article
container_title Current Nutrition & Food Science
container_volume 17
publishDate 2021
institution Swansea University
issn 1573-4013
doi_str_mv 10.2174/1573401317666210803155717
publisher Bentham Science Publishers Ltd.
college_str Swansea University Medical School
hierarchytype
hierarchy_top_id swanseauniversitymedicalschool
hierarchy_top_title Swansea University Medical School
hierarchy_parent_id swanseauniversitymedicalschool
hierarchy_parent_title Swansea University Medical School
department_str Swansea University Medical School{{{_:::_}}}Swansea University Medical School{{{_:::_}}}Swansea University Medical School
document_store_str 0
active_str 0
description Background: Palm oil and its constituents have wide applications in food, cosmetics and pharmaceutical industries. The tocotrienol-rich fractions of crude palm oil have drawn greater research interest in recent years due to its potent health benefits. Therefore, reliable and validated analytical methods are essential for the quantification of tocotrienols.Objective: This study aimed to develop a simple and economical RP-HPLC method for the quantification of gamma-tocotrienol in tocotrienol rich fractions of crude palm oil.Methods: An Agilent HPLC system supplied with a Diode Array detector and an auto-injector system was used for the method development, and the wavelength was set 295 nm. A reversed-phase C18 column maintained at 30 °C using a mobile phase composition of methanol: water (95:05) at a flow rate of 1 mL/min was used for the analysis. The developed method was validated according to ICH guidelines.Results and discussion: A symmetrical peak of gamma-tocotrienol was observed at 8.7 minutes with minimal peak tailing (between 0.76 and 0.78), and an acceptable resolution above 2.0. Excellent linearity was evident with R2 values 0.9996 and 0.9991 for intra-day and inter-day respectively. The method demonstrated a high precision (%RSD values ≤ 5.8%) and accuracy (%RE<9.6%). The LOD and LOQ of gamma-tocotrienol were determined as 1.4 μg/mL and 4.2 μg/mL respectively. The system suitability studies indicate that the chromatographic parameters are well within the acceptable limit.Conclusion: In conclusion, the developed RP-HPLC method is rapid, precise, stable and economical for the quantification of gamma-tocotrienol.
published_date 2021-08-03T04:14:11Z
_version_ 1737027854064943104
score 10.890757