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A validated reverse-phase high performance liquid chromatography (RP-HPLC) method for the quantification of Gamma- tocotrienol in tocotrienol rich fractions of crude palm oil
Current Nutrition & Food Science, Volume: 17
Swansea University Author:
Andrew Morris
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DOI (Published version): 10.2174/1573401317666210803155717
Abstract
Background: Palm oil and its constituents have wide applications in food, cosmetics and pharmaceutical industries. The tocotrienol-rich fractions of crude palm oil have drawn greater research interest in recent years due to its potent health benefits. Therefore, reliable and validated analytical met...
Published in: | Current Nutrition & Food Science |
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ISSN: | 1573-4013 |
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Bentham Science Publishers Ltd.
2021
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URI: | https://cronfa.swan.ac.uk/Record/cronfa57832 |
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2021-11-12T16:24:35.5198814 v2 57832 2021-09-09 A validated reverse-phase high performance liquid chromatography (RP-HPLC) method for the quantification of Gamma- tocotrienol in tocotrienol rich fractions of crude palm oil 3d7a7f540a5143114fcaf67e4c68d151 0000-0002-6315-7553 Andrew Morris Andrew Morris true false 2021-09-09 PHAR Background: Palm oil and its constituents have wide applications in food, cosmetics and pharmaceutical industries. The tocotrienol-rich fractions of crude palm oil have drawn greater research interest in recent years due to its potent health benefits. Therefore, reliable and validated analytical methods are essential for the quantification of tocotrienols.Objective: This study aimed to develop a simple and economical RP-HPLC method for the quantification of gamma-tocotrienol in tocotrienol rich fractions of crude palm oil.Methods: An Agilent HPLC system supplied with a Diode Array detector and an auto-injector system was used for the method development, and the wavelength was set 295 nm. A reversed-phase C18 column maintained at 30 °C using a mobile phase composition of methanol: water (95:05) at a flow rate of 1 mL/min was used for the analysis. The developed method was validated according to ICH guidelines.Results and discussion: A symmetrical peak of gamma-tocotrienol was observed at 8.7 minutes with minimal peak tailing (between 0.76 and 0.78), and an acceptable resolution above 2.0. Excellent linearity was evident with R2 values 0.9996 and 0.9991 for intra-day and inter-day respectively. The method demonstrated a high precision (%RSD values ≤ 5.8%) and accuracy (%RE<9.6%). The LOD and LOQ of gamma-tocotrienol were determined as 1.4 μg/mL and 4.2 μg/mL respectively. The system suitability studies indicate that the chromatographic parameters are well within the acceptable limit.Conclusion: In conclusion, the developed RP-HPLC method is rapid, precise, stable and economical for the quantification of gamma-tocotrienol. Journal Article Current Nutrition & Food Science 17 Bentham Science Publishers Ltd. 1573-4013 TRF, gamma-tocotrienol, RP-HPLC, validation, ICH, system suitability. 3 8 2021 2021-08-03 10.2174/1573401317666210803155717 COLLEGE NANME Pharmacy COLLEGE CODE PHAR Swansea University 2021-11-12T16:24:35.5198814 2021-09-09T22:35:10.6206819 Faculty of Medicine, Health and Life Sciences Swansea University Medical School - Medicine Rajesh Sreedharan Nair 1 Nashiru Billa 2 Andrew Morris 0000-0002-6315-7553 3 57832__20840__0eab8d8eb2814e98a09fdc05c8682423.pdf 57832.pdf 2021-09-13T17:13:39.3246276 Output 530072 application/pdf Accepted Manuscript true 2022-08-03T00:00:00.0000000 true eng |
title |
A validated reverse-phase high performance liquid chromatography (RP-HPLC) method for the quantification of Gamma- tocotrienol in tocotrienol rich fractions of crude palm oil |
spellingShingle |
A validated reverse-phase high performance liquid chromatography (RP-HPLC) method for the quantification of Gamma- tocotrienol in tocotrienol rich fractions of crude palm oil Andrew Morris |
title_short |
A validated reverse-phase high performance liquid chromatography (RP-HPLC) method for the quantification of Gamma- tocotrienol in tocotrienol rich fractions of crude palm oil |
title_full |
A validated reverse-phase high performance liquid chromatography (RP-HPLC) method for the quantification of Gamma- tocotrienol in tocotrienol rich fractions of crude palm oil |
title_fullStr |
A validated reverse-phase high performance liquid chromatography (RP-HPLC) method for the quantification of Gamma- tocotrienol in tocotrienol rich fractions of crude palm oil |
title_full_unstemmed |
A validated reverse-phase high performance liquid chromatography (RP-HPLC) method for the quantification of Gamma- tocotrienol in tocotrienol rich fractions of crude palm oil |
title_sort |
A validated reverse-phase high performance liquid chromatography (RP-HPLC) method for the quantification of Gamma- tocotrienol in tocotrienol rich fractions of crude palm oil |
author_id_str_mv |
3d7a7f540a5143114fcaf67e4c68d151 |
author_id_fullname_str_mv |
3d7a7f540a5143114fcaf67e4c68d151_***_Andrew Morris |
author |
Andrew Morris |
author2 |
Rajesh Sreedharan Nair Nashiru Billa Andrew Morris |
format |
Journal article |
container_title |
Current Nutrition & Food Science |
container_volume |
17 |
publishDate |
2021 |
institution |
Swansea University |
issn |
1573-4013 |
doi_str_mv |
10.2174/1573401317666210803155717 |
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Bentham Science Publishers Ltd. |
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Faculty of Medicine, Health and Life Sciences |
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Faculty of Medicine, Health and Life Sciences |
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Faculty of Medicine, Health and Life Sciences |
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Swansea University Medical School - Medicine{{{_:::_}}}Faculty of Medicine, Health and Life Sciences{{{_:::_}}}Swansea University Medical School - Medicine |
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description |
Background: Palm oil and its constituents have wide applications in food, cosmetics and pharmaceutical industries. The tocotrienol-rich fractions of crude palm oil have drawn greater research interest in recent years due to its potent health benefits. Therefore, reliable and validated analytical methods are essential for the quantification of tocotrienols.Objective: This study aimed to develop a simple and economical RP-HPLC method for the quantification of gamma-tocotrienol in tocotrienol rich fractions of crude palm oil.Methods: An Agilent HPLC system supplied with a Diode Array detector and an auto-injector system was used for the method development, and the wavelength was set 295 nm. A reversed-phase C18 column maintained at 30 °C using a mobile phase composition of methanol: water (95:05) at a flow rate of 1 mL/min was used for the analysis. The developed method was validated according to ICH guidelines.Results and discussion: A symmetrical peak of gamma-tocotrienol was observed at 8.7 minutes with minimal peak tailing (between 0.76 and 0.78), and an acceptable resolution above 2.0. Excellent linearity was evident with R2 values 0.9996 and 0.9991 for intra-day and inter-day respectively. The method demonstrated a high precision (%RSD values ≤ 5.8%) and accuracy (%RE<9.6%). The LOD and LOQ of gamma-tocotrienol were determined as 1.4 μg/mL and 4.2 μg/mL respectively. The system suitability studies indicate that the chromatographic parameters are well within the acceptable limit.Conclusion: In conclusion, the developed RP-HPLC method is rapid, precise, stable and economical for the quantification of gamma-tocotrienol. |
published_date |
2021-08-03T04:13:52Z |
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1763753939539329024 |
score |
11.021648 |