No Cover Image

Journal article 507 views 56 downloads

Role of hypoxia inducible factor 1alpha in cobalt nanoparticle induced cytotoxicity of human THP-1 macrophages

Wendy Francis Orcid Logo, Sian-eleri Owens Orcid Logo, Venkat Kanamarlapudi Orcid Logo, Zhidao Xia Orcid Logo

Biomaterials Translational, Volume: 2, Issue: 2, Pages: 143 - 150

Swansea University Authors: Wendy Francis Orcid Logo, Sian-eleri Owens Orcid Logo, Venkat Kanamarlapudi Orcid Logo, Zhidao Xia Orcid Logo

  • 57975.pdf

    PDF | Version of Record

    Released under the terms of a Creative Commons Attribution-NonCommercial-NoDerivs License (CC BY-NC-ND 4.0)

    Download (1.19MB)

Abstract

Cobalt is one of the main components of metal hip prostheses and cobalt nanoparticles (CoNPs) produced from wear cause inflammation, bone lyses and cytotoxicity at high concentrations. Cobalt ions mimic hypoxia in the presence of normal oxygen levels, and activate hypoxic signalling by stabilising h...

Full description

Published in: Biomaterials Translational
ISSN: 2096-112X
Published: 2021
Online Access: Check full text

URI: https://cronfa.swan.ac.uk/Record/cronfa57975
Tags: Add Tag
No Tags, Be the first to tag this record!
Abstract: Cobalt is one of the main components of metal hip prostheses and cobalt nanoparticles (CoNPs) produced from wear cause inflammation, bone lyses and cytotoxicity at high concentrations. Cobalt ions mimic hypoxia in the presence of normal oxygen levels, and activate hypoxic signalling by stabilising hypoxia inducible transcription factor 1α (HIF1α). This study aimed to assess in vitro the functional role of HIF1α in CoNP induced cellular cytotoxicity. HIF1α, lysosomal pH, tumour necrosis factor α and interleukin 1β expression were analysed in THP-1 macrophages treated with CoNP (0, 10 and 100 μg/mL). HIF1α knock out assays were performed using small interfering RNA to assess the role of HIF1α in CoNP-induced cytotoxicity. Increasing CoNP concentration increased lysosomal activity and acidity in THP-1 macrophages. Higher doses of CoNP significantly reduced cell viability, stimulated caspase 3 activity and apoptosis. Reducing HIF1α activity increased the pro-inflammatory activity of tumour necrosis factor α and interleukin 1β, but had no significant impact on cellular cytotoxicity. This suggests that whilst CoNP promotes cytotoxicity and cellular inflammation, the apoptotic mechanism is not dependent on HIF1α.
Keywords: cobalt nanoparticle, cytotoxicity, hypoxia inducible factor, macrophages, TNFα
College: Faculty of Medicine, Health and Life Sciences
Issue: 2
Start Page: 143
End Page: 150