Journal article 365 views 56 downloads
Metarhizium anisopliae blastospores are highly virulent to adult Aedes aegypti, an important arbovirus vector
Parasites & Vectors, Volume: 14, Issue: 1
Swansea University Author: Tariq Butt
-
PDF | Version of Record
© The Author(s) 2021. Open Access This article is licensed under a Creative Commons Attribution 4.0 International License
Download (910.32KB)
DOI (Published version): 10.1186/s13071-021-05055-z
Abstract
BackgroundThe use of entomopathogenic fungi (EPF) for the control of adult mosquitoes is a promising alternative to synthetic insecticides. Previous studies have only evaluated conidiospores against adult mosquitoes. However, blastospores, which are highly virulent against mosquito larvae and pupae,...
Published in: | Parasites & Vectors |
---|---|
ISSN: | 1756-3305 |
Published: |
Springer Science and Business Media LLC
2021
|
Online Access: |
Check full text
|
URI: | https://cronfa.swan.ac.uk/Record/cronfa58655 |
Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
first_indexed |
2021-11-15T10:25:18Z |
---|---|
last_indexed |
2021-12-01T04:18:38Z |
id |
cronfa58655 |
recordtype |
SURis |
fullrecord |
<?xml version="1.0"?><rfc1807><datestamp>2021-11-30T14:54:59.6627963</datestamp><bib-version>v2</bib-version><id>58655</id><entry>2021-11-15</entry><title>Metarhizium anisopliae blastospores are highly virulent to adult Aedes aegypti, an important arbovirus vector</title><swanseaauthors><author><sid>85d1c2ddde272a1176e74978e25ebece</sid><ORCID>0000-0002-8789-9543</ORCID><firstname>Tariq</firstname><surname>Butt</surname><name>Tariq Butt</name><active>true</active><ethesisStudent>false</ethesisStudent></author></swanseaauthors><date>2021-11-15</date><deptcode>SBI</deptcode><abstract>BackgroundThe use of entomopathogenic fungi (EPF) for the control of adult mosquitoes is a promising alternative to synthetic insecticides. Previous studies have only evaluated conidiospores against adult mosquitoes. However, blastospores, which are highly virulent against mosquito larvae and pupae, could also be effective against adults.MethodsMetarhizium anisopliae (ESALQ 818 and LEF 2000) blastospores and conidia were first tested against adult Aedes aegypti by spraying insects with spore suspensions. Blastospores were then tested using an indirect contact bioassay, exposing mosquitoes to fungus-impregnated cloths. Virulence when using blastospores suspended in 20% sunflower oil was also investigated.ResultsFemale mosquitoes sprayed with blastospores or conidia at a concentration of 108 propagules ml−1 were highly susceptible to both types of spores, resulting in 100% mortality within 7 days. However, significant differences in virulence of the isolates and propagules became apparent at 107 spores ml−1, with ESALQ 818 blastospores being more virulent than LEF 2000 blastospores. ESALQ 818 blastospores were highly virulent when mosquitoes were exposed to black cotton cloths impregnated with blastospores shortly after preparing the suspensions, but virulence declined rapidly 12 h post-application. The addition of vegetable oil to blastospores helped maintain virulence for up to 48 h.ConclusionThe results showed that blastospores were more virulent to adult female Ae. aegypti than conidia when sprayed onto the insects or applied to black cloths. Vegetable oil helped maintain blastospore virulence. The results show that blastospores have potential for use in integrated vector management, although new formulations and drying techniques need to be investigated.</abstract><type>Journal Article</type><journal>Parasites & Vectors</journal><volume>14</volume><journalNumber>1</journalNumber><paginationStart/><paginationEnd/><publisher>Springer Science and Business Media LLC</publisher><placeOfPublication/><isbnPrint/><isbnElectronic/><issnPrint/><issnElectronic>1756-3305</issnElectronic><keywords>Arbovirus, Dengue, Biological control, Fungus, Pathogen, Blastospores, Conidia</keywords><publishedDay>28</publishedDay><publishedMonth>10</publishedMonth><publishedYear>2021</publishedYear><publishedDate>2021-10-28</publishedDate><doi>10.1186/s13071-021-05055-z</doi><url/><notes/><college>COLLEGE NANME</college><department>Biosciences</department><CollegeCode>COLLEGE CODE</CollegeCode><DepartmentCode>SBI</DepartmentCode><institution>Swansea University</institution><apcterm/><funders>This research was funded by FAPERJ: Fundação de Amparo à Pesquisa do Estado do Rio de Janeiro (E-26/201.336/2016; E26/202.923/2019) and CNPQ: Conselho Nacional de Desenvolvimento Científco e Tecnológico (440495/2016)</funders><lastEdited>2021-11-30T14:54:59.6627963</lastEdited><Created>2021-11-15T10:25:10.0664539</Created><path><level id="1">Faculty of Science and Engineering</level><level id="2">School of Biosciences, Geography and Physics - Biosciences</level></path><authors><author><firstname>Adriano Rodrigues de</firstname><surname>Paula</surname><order>1</order></author><author><firstname>Leila Eid Imad</firstname><surname>Silva</surname><order>2</order></author><author><firstname>Anderson</firstname><surname>Ribeiro</surname><order>3</order></author><author><firstname>Gerson Adriano da</firstname><surname>Silva</surname><order>4</order></author><author><firstname>Carlos Peres</firstname><surname>Silva</surname><order>5</order></author><author><firstname>Tariq</firstname><surname>Butt</surname><orcid>0000-0002-8789-9543</orcid><order>6</order></author><author><firstname>Richard Ian</firstname><surname>Samuels</surname><order>7</order></author></authors><documents><document><filename>58655__21733__d6a5c9e87f4d4db98100705f28b3a461.pdf</filename><originalFilename>58655.pdf</originalFilename><uploaded>2021-11-30T14:53:50.6981927</uploaded><type>Output</type><contentLength>932164</contentLength><contentType>application/pdf</contentType><version>Version of Record</version><cronfaStatus>true</cronfaStatus><documentNotes>© The Author(s) 2021. Open Access This article is licensed under a Creative Commons Attribution 4.0 International License</documentNotes><copyrightCorrect>true</copyrightCorrect><language>eng</language><licence>http://creativecommons.org/licenses/by/4.0/</licence></document></documents><OutputDurs/></rfc1807> |
spelling |
2021-11-30T14:54:59.6627963 v2 58655 2021-11-15 Metarhizium anisopliae blastospores are highly virulent to adult Aedes aegypti, an important arbovirus vector 85d1c2ddde272a1176e74978e25ebece 0000-0002-8789-9543 Tariq Butt Tariq Butt true false 2021-11-15 SBI BackgroundThe use of entomopathogenic fungi (EPF) for the control of adult mosquitoes is a promising alternative to synthetic insecticides. Previous studies have only evaluated conidiospores against adult mosquitoes. However, blastospores, which are highly virulent against mosquito larvae and pupae, could also be effective against adults.MethodsMetarhizium anisopliae (ESALQ 818 and LEF 2000) blastospores and conidia were first tested against adult Aedes aegypti by spraying insects with spore suspensions. Blastospores were then tested using an indirect contact bioassay, exposing mosquitoes to fungus-impregnated cloths. Virulence when using blastospores suspended in 20% sunflower oil was also investigated.ResultsFemale mosquitoes sprayed with blastospores or conidia at a concentration of 108 propagules ml−1 were highly susceptible to both types of spores, resulting in 100% mortality within 7 days. However, significant differences in virulence of the isolates and propagules became apparent at 107 spores ml−1, with ESALQ 818 blastospores being more virulent than LEF 2000 blastospores. ESALQ 818 blastospores were highly virulent when mosquitoes were exposed to black cotton cloths impregnated with blastospores shortly after preparing the suspensions, but virulence declined rapidly 12 h post-application. The addition of vegetable oil to blastospores helped maintain virulence for up to 48 h.ConclusionThe results showed that blastospores were more virulent to adult female Ae. aegypti than conidia when sprayed onto the insects or applied to black cloths. Vegetable oil helped maintain blastospore virulence. The results show that blastospores have potential for use in integrated vector management, although new formulations and drying techniques need to be investigated. Journal Article Parasites & Vectors 14 1 Springer Science and Business Media LLC 1756-3305 Arbovirus, Dengue, Biological control, Fungus, Pathogen, Blastospores, Conidia 28 10 2021 2021-10-28 10.1186/s13071-021-05055-z COLLEGE NANME Biosciences COLLEGE CODE SBI Swansea University This research was funded by FAPERJ: Fundação de Amparo à Pesquisa do Estado do Rio de Janeiro (E-26/201.336/2016; E26/202.923/2019) and CNPQ: Conselho Nacional de Desenvolvimento Científco e Tecnológico (440495/2016) 2021-11-30T14:54:59.6627963 2021-11-15T10:25:10.0664539 Faculty of Science and Engineering School of Biosciences, Geography and Physics - Biosciences Adriano Rodrigues de Paula 1 Leila Eid Imad Silva 2 Anderson Ribeiro 3 Gerson Adriano da Silva 4 Carlos Peres Silva 5 Tariq Butt 0000-0002-8789-9543 6 Richard Ian Samuels 7 58655__21733__d6a5c9e87f4d4db98100705f28b3a461.pdf 58655.pdf 2021-11-30T14:53:50.6981927 Output 932164 application/pdf Version of Record true © The Author(s) 2021. Open Access This article is licensed under a Creative Commons Attribution 4.0 International License true eng http://creativecommons.org/licenses/by/4.0/ |
title |
Metarhizium anisopliae blastospores are highly virulent to adult Aedes aegypti, an important arbovirus vector |
spellingShingle |
Metarhizium anisopliae blastospores are highly virulent to adult Aedes aegypti, an important arbovirus vector Tariq Butt |
title_short |
Metarhizium anisopliae blastospores are highly virulent to adult Aedes aegypti, an important arbovirus vector |
title_full |
Metarhizium anisopliae blastospores are highly virulent to adult Aedes aegypti, an important arbovirus vector |
title_fullStr |
Metarhizium anisopliae blastospores are highly virulent to adult Aedes aegypti, an important arbovirus vector |
title_full_unstemmed |
Metarhizium anisopliae blastospores are highly virulent to adult Aedes aegypti, an important arbovirus vector |
title_sort |
Metarhizium anisopliae blastospores are highly virulent to adult Aedes aegypti, an important arbovirus vector |
author_id_str_mv |
85d1c2ddde272a1176e74978e25ebece |
author_id_fullname_str_mv |
85d1c2ddde272a1176e74978e25ebece_***_Tariq Butt |
author |
Tariq Butt |
author2 |
Adriano Rodrigues de Paula Leila Eid Imad Silva Anderson Ribeiro Gerson Adriano da Silva Carlos Peres Silva Tariq Butt Richard Ian Samuels |
format |
Journal article |
container_title |
Parasites & Vectors |
container_volume |
14 |
container_issue |
1 |
publishDate |
2021 |
institution |
Swansea University |
issn |
1756-3305 |
doi_str_mv |
10.1186/s13071-021-05055-z |
publisher |
Springer Science and Business Media LLC |
college_str |
Faculty of Science and Engineering |
hierarchytype |
|
hierarchy_top_id |
facultyofscienceandengineering |
hierarchy_top_title |
Faculty of Science and Engineering |
hierarchy_parent_id |
facultyofscienceandengineering |
hierarchy_parent_title |
Faculty of Science and Engineering |
department_str |
School of Biosciences, Geography and Physics - Biosciences{{{_:::_}}}Faculty of Science and Engineering{{{_:::_}}}School of Biosciences, Geography and Physics - Biosciences |
document_store_str |
1 |
active_str |
0 |
description |
BackgroundThe use of entomopathogenic fungi (EPF) for the control of adult mosquitoes is a promising alternative to synthetic insecticides. Previous studies have only evaluated conidiospores against adult mosquitoes. However, blastospores, which are highly virulent against mosquito larvae and pupae, could also be effective against adults.MethodsMetarhizium anisopliae (ESALQ 818 and LEF 2000) blastospores and conidia were first tested against adult Aedes aegypti by spraying insects with spore suspensions. Blastospores were then tested using an indirect contact bioassay, exposing mosquitoes to fungus-impregnated cloths. Virulence when using blastospores suspended in 20% sunflower oil was also investigated.ResultsFemale mosquitoes sprayed with blastospores or conidia at a concentration of 108 propagules ml−1 were highly susceptible to both types of spores, resulting in 100% mortality within 7 days. However, significant differences in virulence of the isolates and propagules became apparent at 107 spores ml−1, with ESALQ 818 blastospores being more virulent than LEF 2000 blastospores. ESALQ 818 blastospores were highly virulent when mosquitoes were exposed to black cotton cloths impregnated with blastospores shortly after preparing the suspensions, but virulence declined rapidly 12 h post-application. The addition of vegetable oil to blastospores helped maintain virulence for up to 48 h.ConclusionThe results showed that blastospores were more virulent to adult female Ae. aegypti than conidia when sprayed onto the insects or applied to black cloths. Vegetable oil helped maintain blastospore virulence. The results show that blastospores have potential for use in integrated vector management, although new formulations and drying techniques need to be investigated. |
published_date |
2021-10-28T04:15:21Z |
_version_ |
1763754032426385408 |
score |
10.99342 |