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Looking through the FOG: microbiome characterization and lipolytic bacteria isolation from a fatberg site

Elizabeth K. Court, Roy R. Chaudhuri, Rahul Kapoore Orcid Logo, Raffaella X. Villa, Jagroop Pandhal, Catherine A. Biggs, Graham P. Stafford

Microbiology, Volume: 167, Issue: 12

Swansea University Author: Rahul Kapoore Orcid Logo

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DOI (Published version): 10.1099/mic.0.001117

Abstract

Sewer systems are complex physical, chemical and microbial ecosystems where fats, oils and grease (FOG) present a major problem for sewer management. Their accumulation can lead to blockages (‘Fatbergs’), sewer overflows and disruption of downstream wastewater treatment. Further advancements of biol...

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Published in: Microbiology
ISSN: 1350-0872 1465-2080
Published: Microbiology Society 2021
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URI: https://cronfa.swan.ac.uk/Record/cronfa59015
first_indexed 2021-12-15T17:11:55Z
last_indexed 2021-12-16T04:28:20Z
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spelling 2021-12-15T17:14:05.4866309 v2 59015 2021-12-15 Looking through the FOG: microbiome characterization and lipolytic bacteria isolation from a fatberg site 5583be4600daecd670edac16f6e77e88 0000-0002-2287-0619 Rahul Kapoore Rahul Kapoore true false 2021-12-15 BGPS Sewer systems are complex physical, chemical and microbial ecosystems where fats, oils and grease (FOG) present a major problem for sewer management. Their accumulation can lead to blockages (‘Fatbergs’), sewer overflows and disruption of downstream wastewater treatment. Further advancements of biological FOG treatments need to be tailored to degrade the FOG, and operate successfully within the sewer environment. In this study we developed a pipeline for isolation of lipolytic strains directly from two FOG blockage sites in the UK, and isolated a range of highly lipolytic bacteria. We selected the five most lipolytic strains using Rhodamine B agar plates and pNP-Fatty acid substrates, with two Serratia spp., two Klebsiella spp. and an environmental Acinetobacter strain that all have the capacity to grow on FOG-based carbon sources. Their genome sequences identified the genetic capacity for fatty acid harvesting (lipases), catabolism and utilization (Fad genes). Furthermore, we performed a preliminary molecular characterization of the microbial community at these sites, showing a diverse community of environmental bacteria at each site, but which did include evidence of sequences related to our isolates. This study provides proof of concept to isolation strategies targeting Fatberg sites to yield candidate strains with bioremediation potential for FOG in the wastewater network. Our work sets the foundation for development of novel bioadditions tailored to the environment with non-pathogenic Acinetobacter identified as a candidate for this purpose. Journal Article Microbiology 167 12 Microbiology Society 1350-0872 1465-2080 wastewater, fatberg, FOG, fat oil and grease, microbial communities 6 12 2021 2021-12-06 10.1099/mic.0.001117 COLLEGE NANME Biosciences Geography and Physics School COLLEGE CODE BGPS Swansea University UK Biotechnology and Biological Sciences Research Council (grant number BB/L024209/1); UK Engineering and Physical Sciences Research Council (EP/N010124/1) the University of Sheffield Impact and Innovation Knowledge Exchange (156922). 2021-12-15T17:14:05.4866309 2021-12-15T17:08:52.3042684 Faculty of Science and Engineering School of Biosciences, Geography and Physics - Biosciences Elizabeth K. Court 1 Roy R. Chaudhuri 2 Rahul Kapoore 0000-0002-2287-0619 3 Raffaella X. Villa 4 Jagroop Pandhal 5 Catherine A. Biggs 6 Graham P. Stafford 7 59015__21897__4a8d4c65a1644b86af3c666756551c66.pdf 59015.pdf 2021-12-15T17:12:26.6474761 Output 2375447 application/pdf Version of Record true © 2021 The Authors. This is an open-access article distributed under the terms of the Creative Commons Attribution License true eng https://creativecommons.org/licenses/by/4.0/deed.ast
title Looking through the FOG: microbiome characterization and lipolytic bacteria isolation from a fatberg site
spellingShingle Looking through the FOG: microbiome characterization and lipolytic bacteria isolation from a fatberg site
Rahul Kapoore
title_short Looking through the FOG: microbiome characterization and lipolytic bacteria isolation from a fatberg site
title_full Looking through the FOG: microbiome characterization and lipolytic bacteria isolation from a fatberg site
title_fullStr Looking through the FOG: microbiome characterization and lipolytic bacteria isolation from a fatberg site
title_full_unstemmed Looking through the FOG: microbiome characterization and lipolytic bacteria isolation from a fatberg site
title_sort Looking through the FOG: microbiome characterization and lipolytic bacteria isolation from a fatberg site
author_id_str_mv 5583be4600daecd670edac16f6e77e88
author_id_fullname_str_mv 5583be4600daecd670edac16f6e77e88_***_Rahul Kapoore
author Rahul Kapoore
author2 Elizabeth K. Court
Roy R. Chaudhuri
Rahul Kapoore
Raffaella X. Villa
Jagroop Pandhal
Catherine A. Biggs
Graham P. Stafford
format Journal article
container_title Microbiology
container_volume 167
container_issue 12
publishDate 2021
institution Swansea University
issn 1350-0872
1465-2080
doi_str_mv 10.1099/mic.0.001117
publisher Microbiology Society
college_str Faculty of Science and Engineering
hierarchytype
hierarchy_top_id facultyofscienceandengineering
hierarchy_top_title Faculty of Science and Engineering
hierarchy_parent_id facultyofscienceandengineering
hierarchy_parent_title Faculty of Science and Engineering
department_str School of Biosciences, Geography and Physics - Biosciences{{{_:::_}}}Faculty of Science and Engineering{{{_:::_}}}School of Biosciences, Geography and Physics - Biosciences
document_store_str 1
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description Sewer systems are complex physical, chemical and microbial ecosystems where fats, oils and grease (FOG) present a major problem for sewer management. Their accumulation can lead to blockages (‘Fatbergs’), sewer overflows and disruption of downstream wastewater treatment. Further advancements of biological FOG treatments need to be tailored to degrade the FOG, and operate successfully within the sewer environment. In this study we developed a pipeline for isolation of lipolytic strains directly from two FOG blockage sites in the UK, and isolated a range of highly lipolytic bacteria. We selected the five most lipolytic strains using Rhodamine B agar plates and pNP-Fatty acid substrates, with two Serratia spp., two Klebsiella spp. and an environmental Acinetobacter strain that all have the capacity to grow on FOG-based carbon sources. Their genome sequences identified the genetic capacity for fatty acid harvesting (lipases), catabolism and utilization (Fad genes). Furthermore, we performed a preliminary molecular characterization of the microbial community at these sites, showing a diverse community of environmental bacteria at each site, but which did include evidence of sequences related to our isolates. This study provides proof of concept to isolation strategies targeting Fatberg sites to yield candidate strains with bioremediation potential for FOG in the wastewater network. Our work sets the foundation for development of novel bioadditions tailored to the environment with non-pathogenic Acinetobacter identified as a candidate for this purpose.
published_date 2021-12-06T20:08:34Z
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