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Characterization and <i>ex vivo</i> evaluation of curcumin nanoethosomes for melanoma treatment

Rajesh Sreedharan Nair Orcid Logo, Nashiru Billa Orcid Logo, Lim Yang Mooi Orcid Logo, Andrew Morris Orcid Logo

Pharmaceutical Development and Technology, Volume: 27, Issue: 1, Pages: 72 - 82

Swansea University Author: Andrew Morris Orcid Logo

  • Accepted Manuscript under embargo until: 27th December 2022

Abstract

This study aimed at developing curcumin nanoethosomes (Cur-Ets) with superior skin permeation intended for melanoma treatment. Although curcumin is active against many types of skin cancers, a suitable topical formulation is still lacking due to its hydrophobicity and poor skin permeation. The formu...

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Published in: Pharmaceutical Development and Technology
ISSN: 1083-7450 1097-9867
Published: Informa UK Limited 2022
Online Access: Check full text

URI: https://cronfa.swan.ac.uk/Record/cronfa59051
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Abstract: This study aimed at developing curcumin nanoethosomes (Cur-Ets) with superior skin permeation intended for melanoma treatment. Although curcumin is active against many types of skin cancers, a suitable topical formulation is still lacking due to its hydrophobicity and poor skin permeation. The formulation was characterized using Scanning Transmission Electron Microscopy (STEM), atomic force microscopy (AFM), ATR-FTIR, DSC, and XRD. In vitro skin permeation was carried out using human skin, and the cytotoxicity of the formulation was evaluated on human melanoma cells (SK-MEL28). The vesicle size and zeta potential of the Cur-Ets were determined as 67 ± 1.6 nm and - 87.3 ± 3.3 mV, respectively. STEM and AFM analysis further support the size and morphology of the formulation. Curcumin's compatibility with formulation additives was confirmed by ATR-FTIR analysis. In addition, DSC and XRD analyses showed successful drug encapsulation in nanoethosomes. The drug encapsulation efficiency was determined as 87 ± 0.9%. The skin permeation of curcumin from Cur-Ets showed a superior flux (0.14 ± 0.03 µg cm−2 h−1) compared to the control (p < 0.05). Cytotoxicity of the formulation demonstrated a time-dependent and concentration-dependent antiproliferative activity against melanoma cells. The developed Cur-Ets is suggested as a promising topical formulation for melanoma treatment.
Keywords: curcumin; melanoma; nanoethosomes; human skin; permeation; cytotoxicity
College: Swansea University Medical School
Issue: 1
Start Page: 72
End Page: 82