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Early pre-neural serotonin modulates balance of late monoamines and behavioral patterns in fish model system

Evgeny Ivashkin Orcid Logo, Stefan Spulber, Andrei Zinovyev, Takashi Yoshitake, Shimako Yoshitake, Olga Kharchenko, Marina Yu. Khabarova, Spyridon Theofilopoulos Orcid Logo, Jan Kehr, Ernest Arenas, Sandra Ceccatelli, Elena E. Voronezhskaya, Igor Adameyko

BioRxiv

Swansea University Author: Spyridon Theofilopoulos Orcid Logo

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DOI (Published version): 10.1101/2022.07.25.501403

Abstract

The presence of serotonergic system during early pre-neural development is enigmatic and conserved amongst all studied invertebrate and vertebrate animals. We took advantage of zebrafish model system to address what is the role of early serotonin before first neurons form. Unexpectedly, we experimen...

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Published in: BioRxiv
Published: Cold Spring Harbor Laboratory 2022
URI: https://cronfa.swan.ac.uk/Record/cronfa60878
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first_indexed 2022-08-24T07:49:34Z
last_indexed 2023-01-13T19:21:20Z
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spelling v2 60878 2022-08-24 Early pre-neural serotonin modulates balance of late monoamines and behavioral patterns in fish model system bcd1bdbdf59d0724d2f9e9a48e671107 0000-0003-1986-0943 Spyridon Theofilopoulos Spyridon Theofilopoulos true false 2022-08-24 BMS The presence of serotonergic system during early pre-neural development is enigmatic and conserved amongst all studied invertebrate and vertebrate animals. We took advantage of zebrafish model system to address what is the role of early serotonin before first neurons form. Unexpectedly, we experimentally revealed the existence of delayed developmental neurogenic and behavioral effects resulting from the manipulations of pre-neural (zygote, blastula and gastrula) serotonergic system. In particular, the delayed effects included differences in the synthesis of serotonin in early serotonergic neurons in the central nervous system as well as in behavioral alterations after habituation in zebrafish larvae. These effects appeared as highly specific and did not coincide with any major abnormalities. The same manipulations of the serotonergic system at neural developmental stages did not show such effects, which confirms that early effects of serotonergic system manipulation are not based on retained serotonin in embryonic cells. Accordingly, gene expression analysis demonstrated specific changes only in response to the elevation of early pre-neural serotonin, which included the delayed and pre-mature onsets of different gene expression programs. Taken together, our results introduce a novel function of early pre-neural serotonergic system in a vertebrate embryo – tuning and fine control of specific mechanisms at later neural developmental stages that result in a mild variation of a behavioral adaptive spectrum. Journal Article BioRxiv Cold Spring Harbor Laboratory 26 7 2022 2022-07-26 10.1101/2022.07.25.501403 Preprint article before certification by peer review. COLLEGE NANME Biomedical Sciences COLLEGE CODE BMS Swansea University 2023-06-01T11:26:40.2364879 2022-08-24T08:43:08.7980734 Faculty of Medicine, Health and Life Sciences Swansea University Medical School - Medicine Evgeny Ivashkin 0000-0002-1763-0282 1 Stefan Spulber 2 Andrei Zinovyev 3 Takashi Yoshitake 4 Shimako Yoshitake 5 Olga Kharchenko 6 Marina Yu. Khabarova 7 Spyridon Theofilopoulos 0000-0003-1986-0943 8 Jan Kehr 9 Ernest Arenas 10 Sandra Ceccatelli 11 Elena E. Voronezhskaya 12 Igor Adameyko 13
title Early pre-neural serotonin modulates balance of late monoamines and behavioral patterns in fish model system
spellingShingle Early pre-neural serotonin modulates balance of late monoamines and behavioral patterns in fish model system
Spyridon Theofilopoulos
title_short Early pre-neural serotonin modulates balance of late monoamines and behavioral patterns in fish model system
title_full Early pre-neural serotonin modulates balance of late monoamines and behavioral patterns in fish model system
title_fullStr Early pre-neural serotonin modulates balance of late monoamines and behavioral patterns in fish model system
title_full_unstemmed Early pre-neural serotonin modulates balance of late monoamines and behavioral patterns in fish model system
title_sort Early pre-neural serotonin modulates balance of late monoamines and behavioral patterns in fish model system
author_id_str_mv bcd1bdbdf59d0724d2f9e9a48e671107
author_id_fullname_str_mv bcd1bdbdf59d0724d2f9e9a48e671107_***_Spyridon Theofilopoulos
author Spyridon Theofilopoulos
author2 Evgeny Ivashkin
Stefan Spulber
Andrei Zinovyev
Takashi Yoshitake
Shimako Yoshitake
Olga Kharchenko
Marina Yu. Khabarova
Spyridon Theofilopoulos
Jan Kehr
Ernest Arenas
Sandra Ceccatelli
Elena E. Voronezhskaya
Igor Adameyko
format Journal article
container_title BioRxiv
publishDate 2022
institution Swansea University
doi_str_mv 10.1101/2022.07.25.501403
publisher Cold Spring Harbor Laboratory
college_str Faculty of Medicine, Health and Life Sciences
hierarchytype
hierarchy_top_id facultyofmedicinehealthandlifesciences
hierarchy_top_title Faculty of Medicine, Health and Life Sciences
hierarchy_parent_id facultyofmedicinehealthandlifesciences
hierarchy_parent_title Faculty of Medicine, Health and Life Sciences
department_str Swansea University Medical School - Medicine{{{_:::_}}}Faculty of Medicine, Health and Life Sciences{{{_:::_}}}Swansea University Medical School - Medicine
document_store_str 0
active_str 0
description The presence of serotonergic system during early pre-neural development is enigmatic and conserved amongst all studied invertebrate and vertebrate animals. We took advantage of zebrafish model system to address what is the role of early serotonin before first neurons form. Unexpectedly, we experimentally revealed the existence of delayed developmental neurogenic and behavioral effects resulting from the manipulations of pre-neural (zygote, blastula and gastrula) serotonergic system. In particular, the delayed effects included differences in the synthesis of serotonin in early serotonergic neurons in the central nervous system as well as in behavioral alterations after habituation in zebrafish larvae. These effects appeared as highly specific and did not coincide with any major abnormalities. The same manipulations of the serotonergic system at neural developmental stages did not show such effects, which confirms that early effects of serotonergic system manipulation are not based on retained serotonin in embryonic cells. Accordingly, gene expression analysis demonstrated specific changes only in response to the elevation of early pre-neural serotonin, which included the delayed and pre-mature onsets of different gene expression programs. Taken together, our results introduce a novel function of early pre-neural serotonergic system in a vertebrate embryo – tuning and fine control of specific mechanisms at later neural developmental stages that result in a mild variation of a behavioral adaptive spectrum.
published_date 2022-07-26T11:26:39Z
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score 11.016503

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