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The tetrameric pheromone module SteC-MkkB-MpkB-SteD regulates asexual sporulation, sclerotia formation and aflatoxin production in Aspergillus flavus

Dean Frawley Orcid Logo, Claudio Greco Orcid Logo, Berl Oakley, Mohamed M. Alhussain, Alastair B. Fleming, Nancy P. Keller Orcid Logo, Özgür Bayram Orcid Logo

Cellular Microbiology, Volume: 22, Issue: 6

Swansea University Author: Claudio Greco Orcid Logo

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DOI (Published version): 10.1111/cmi.13192

Abstract

For eukaryotes like fungi to regulate biological responses to environmental stimuli, various signalling cascades are utilized, like the highly conserved mitogen-activated protein kinase (MAPK) pathways. In the model fungus Aspergillus nidulans, a MAPK pathway known as the pheromone module regulates...

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Published in: Cellular Microbiology
ISSN: 1462-5814 1462-5822
Published: Hindawi Limited 2020
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URI: https://cronfa.swan.ac.uk/Record/cronfa61520
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spelling 2022-10-20T13:05:45.2489929 v2 61520 2022-10-10 The tetrameric pheromone module SteC-MkkB-MpkB-SteD regulates asexual sporulation, sclerotia formation and aflatoxin production in Aspergillus flavus cacac6459bd7cf4a241f63661006036f 0000-0003-3067-0999 Claudio Greco Claudio Greco true false 2022-10-10 BGPS For eukaryotes like fungi to regulate biological responses to environmental stimuli, various signalling cascades are utilized, like the highly conserved mitogen-activated protein kinase (MAPK) pathways. In the model fungus Aspergillus nidulans, a MAPK pathway known as the pheromone module regulates development and the production of secondary metabolites (SMs). This pathway consists five proteins, the three kinases SteC, MkkB and MpkB, the adaptor SteD and the scaffold HamE. In this study, homologs of these five pheromone module proteins have been identified in the plant and human pathogenic fungus Aspergillus flavus. We have shown that a tetrameric complex consisting of the three kinases and the SteD adaptor is assembled in this species. It was observed that this complex assembles in the cytoplasm and that MpkB translocates into the nucleus. Deletion of steC, mkkB, mpkB or steD results in abolishment of both asexual sporulation and sclerotia production. This complex is required for the positive regulation of aflatoxin production and negative regulation of various SMs, including leporin B and cyclopiazonic acid (CPA), likely via MpkB interactions in the nucleus. These data highlight the conservation of the pheromone module in Aspergillus species, signifying the importance of this pathway in regulating fungal development and secondary metabolism. Journal Article Cellular Microbiology 22 6 Hindawi Limited 1462-5814 1462-5822 aflatoxin B1,Aspergillus flavus, pheromone module, sclerotia, secondary metabolism 1 6 2020 2020-06-01 10.1111/cmi.13192 COLLEGE NANME Biosciences Geography and Physics School COLLEGE CODE BGPS Swansea University rish Research Council for Science, Engineeringand Technology, Grant/Award Number:GOIPG/2018/35; Irving S. Johnson Fund ofthe University of Kansas Foundation; John andPat Hume Scholarship; NIH Clinical Center,Grant/Award Number: R01GM112739;Science Foundation Ireland, Grant/AwardNumbers: 12/RI/2346(3), 13/CDA/2142,SFI/07/RFP/GEN/F571/ECO7 2022-10-20T13:05:45.2489929 2022-10-10T17:24:49.5951830 Faculty of Science and Engineering School of Biosciences, Geography and Physics - Biosciences Dean Frawley 0000-0002-8371-5233 1 Claudio Greco 0000-0003-3067-0999 2 Berl Oakley 3 Mohamed M. Alhussain 4 Alastair B. Fleming 5 Nancy P. Keller 0000-0002-4386-9473 6 Özgür Bayram 0000-0002-0283-5322 7 61520__25519__0b339cff6fea43bcb4fdc377bd9d23a0.pdf 61520_VoR.pdf 2022-10-20T13:01:46.1775600 Output 3580439 application/pdf Version of Record true © 2020 The Authors. This is an open access article under the terms of the Creative Commons Attribution License true eng http://creativecommons.org/licenses/by/4.0/
title The tetrameric pheromone module SteC-MkkB-MpkB-SteD regulates asexual sporulation, sclerotia formation and aflatoxin production in Aspergillus flavus
spellingShingle The tetrameric pheromone module SteC-MkkB-MpkB-SteD regulates asexual sporulation, sclerotia formation and aflatoxin production in Aspergillus flavus
Claudio Greco
title_short The tetrameric pheromone module SteC-MkkB-MpkB-SteD regulates asexual sporulation, sclerotia formation and aflatoxin production in Aspergillus flavus
title_full The tetrameric pheromone module SteC-MkkB-MpkB-SteD regulates asexual sporulation, sclerotia formation and aflatoxin production in Aspergillus flavus
title_fullStr The tetrameric pheromone module SteC-MkkB-MpkB-SteD regulates asexual sporulation, sclerotia formation and aflatoxin production in Aspergillus flavus
title_full_unstemmed The tetrameric pheromone module SteC-MkkB-MpkB-SteD regulates asexual sporulation, sclerotia formation and aflatoxin production in Aspergillus flavus
title_sort The tetrameric pheromone module SteC-MkkB-MpkB-SteD regulates asexual sporulation, sclerotia formation and aflatoxin production in Aspergillus flavus
author_id_str_mv cacac6459bd7cf4a241f63661006036f
author_id_fullname_str_mv cacac6459bd7cf4a241f63661006036f_***_Claudio Greco
author Claudio Greco
author2 Dean Frawley
Claudio Greco
Berl Oakley
Mohamed M. Alhussain
Alastair B. Fleming
Nancy P. Keller
Özgür Bayram
format Journal article
container_title Cellular Microbiology
container_volume 22
container_issue 6
publishDate 2020
institution Swansea University
issn 1462-5814
1462-5822
doi_str_mv 10.1111/cmi.13192
publisher Hindawi Limited
college_str Faculty of Science and Engineering
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hierarchy_top_title Faculty of Science and Engineering
hierarchy_parent_id facultyofscienceandengineering
hierarchy_parent_title Faculty of Science and Engineering
department_str School of Biosciences, Geography and Physics - Biosciences{{{_:::_}}}Faculty of Science and Engineering{{{_:::_}}}School of Biosciences, Geography and Physics - Biosciences
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description For eukaryotes like fungi to regulate biological responses to environmental stimuli, various signalling cascades are utilized, like the highly conserved mitogen-activated protein kinase (MAPK) pathways. In the model fungus Aspergillus nidulans, a MAPK pathway known as the pheromone module regulates development and the production of secondary metabolites (SMs). This pathway consists five proteins, the three kinases SteC, MkkB and MpkB, the adaptor SteD and the scaffold HamE. In this study, homologs of these five pheromone module proteins have been identified in the plant and human pathogenic fungus Aspergillus flavus. We have shown that a tetrameric complex consisting of the three kinases and the SteD adaptor is assembled in this species. It was observed that this complex assembles in the cytoplasm and that MpkB translocates into the nucleus. Deletion of steC, mkkB, mpkB or steD results in abolishment of both asexual sporulation and sclerotia production. This complex is required for the positive regulation of aflatoxin production and negative regulation of various SMs, including leporin B and cyclopiazonic acid (CPA), likely via MpkB interactions in the nucleus. These data highlight the conservation of the pheromone module in Aspergillus species, signifying the importance of this pathway in regulating fungal development and secondary metabolism.
published_date 2020-06-01T20:28:53Z
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