Marine Chitinolytic Pseudoalteromonas Represents an Untapped Reservoir of Bioactive Potential

Paulsen, Sara Skøtt; Strube, Mikael Lenz; Bech, Pernille Kjersgaard; Gram, Lone; Sonnenschein, Eva C.

doi:10.1128/msystems.00060-19

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Marine Chitinolytic Pseudoalteromonas Represents an Untapped Reservoir of Bioactive Potential

Sara Skøtt Paulsen

, Mikael Lenz Strube

, Pernille Kjersgaard Bech

, Lone Gram

, Eva C. Sonnenschein

mSystems, Volume: 4, Issue: 4

Swansea University Author: Eva C. Sonnenschein

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DOI (Published version): 10.1128/msystems.00060-19

Abstract

Chitin is the most abundant polymer in the marine environment and a nutrient-rich surface for adhering marine bacteria. We have previously shown that chitin can induce the production of antibiotic compounds in Vibrionaceae, suggesting that the discovery of novel bioactive molecules from bacteria can...

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Published in:	mSystems
ISSN:	2379-5077
Published:	American Society for Microbiology 2019
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URI:	https://cronfa.swan.ac.uk/Record/cronfa61726
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spelling	2022-11-04T17:26:40.4720886 v2 61726 2022-10-31 Marine Chitinolytic Pseudoalteromonas Represents an Untapped Reservoir of Bioactive Potential f6a4027578a15ea3e6453a54b849c686 0000-0001-6959-5100 Eva C. Sonnenschein Eva C. Sonnenschein true false 2022-10-31 SBI Chitin is the most abundant polymer in the marine environment and a nutrient-rich surface for adhering marine bacteria. We have previously shown that chitin can induce the production of antibiotic compounds in Vibrionaceae, suggesting that the discovery of novel bioactive molecules from bacteria can be facilitated by mimicking their natural habitat. The purpose of this study was to determine the glycosyl hydrolase (GH) profiles of strains of the genus Pseudoalteromonas to enable selection of presumed growth substrates and explore possible links to secondary metabolism. Genomic analyses were conducted on 62 pigmented and 95 nonpigmented strains. Analysis of the total GH profiles and multidimensional scaling suggested that the degradation of chitin is a significant trait of pigmented strains, whereas nonpigmented strains seem to be driven toward the degradation of alga-derived carbohydrates. The genomes of all pigmented strains and 40 nonpigmented strains encoded at least one conserved chitin degradation cluster, and chitinolytic activity was phenotypically confirmed. Additionally, the genomes of all pigmented and a few nonpigmented strains encoded chitinases of the rare GH family 19. Pigmented strains devote up to 15% of their genome to secondary metabolism, while for nonpigmented species it was 3% at most. Thus, pigmented Pseudoalteromonas strains have a bioactive potential similar to that of well-known antibiotic producers of the Actinobacteria phylum. Growth on chitin did not measurably enhance the antibacterial activity of the strains; however, we demonstrated a remarkable co-occurrence of chitin degradation and the potential for secondary metabolite production in pigmented Pseudoalteromonas strains. This indicates that chitin and its colonizers of the Pseudoalteromonas genus represent a so far underexplored niche for novel enzymes and bioactive compounds. Journal Article mSystems 4 4 American Society for Microbiology 2379-5077 bioactivity, glycosyl hydrolases, Pseudoalteromonas, chitin 27 8 2019 2019-08-27 10.1128/msystems.00060-19 COLLEGE NANME Biosciences COLLEGE CODE SBI Swansea University The study was funded by the Villum Kann Rasmussen Annual Award 2016 to Lone Gram. 2022-11-04T17:26:40.4720886 2022-10-31T15:19:12.1168137 Faculty of Science and Engineering School of Biosciences, Geography and Physics - Biosciences Sara Skøtt Paulsen 0000-0002-5610-5915 1 Mikael Lenz Strube 0000-0003-0905-5705 2 Pernille Kjersgaard Bech 0000-0002-6028-9382 3 Lone Gram 0000-0002-1076-5723 4 Eva C. Sonnenschein 0000-0001-6959-5100 5 61726__25655__cc1f1b67bf094b089aa7f94b452206a0.pdf 61726.pdf 2022-11-04T17:25:22.2860655 Output 2912847 application/pdf Version of Record true © 2019 Paulsen et al. This is an open-access article distributed under the terms of the Creative Commons Attribution 4.0 International license true eng https://creativecommons.org/licenses/by/4.0/
title	Marine Chitinolytic Pseudoalteromonas Represents an Untapped Reservoir of Bioactive Potential
spellingShingle	Marine Chitinolytic Pseudoalteromonas Represents an Untapped Reservoir of Bioactive Potential Eva C. Sonnenschein
title_short	Marine Chitinolytic Pseudoalteromonas Represents an Untapped Reservoir of Bioactive Potential
title_full	Marine Chitinolytic Pseudoalteromonas Represents an Untapped Reservoir of Bioactive Potential
title_fullStr	Marine Chitinolytic Pseudoalteromonas Represents an Untapped Reservoir of Bioactive Potential
title_full_unstemmed	Marine Chitinolytic Pseudoalteromonas Represents an Untapped Reservoir of Bioactive Potential
title_sort	Marine Chitinolytic Pseudoalteromonas Represents an Untapped Reservoir of Bioactive Potential
author_id_str_mv	f6a4027578a15ea3e6453a54b849c686
author_id_fullname_str_mv	f6a4027578a15ea3e6453a54b849c686_***_Eva C. Sonnenschein
author	Eva C. Sonnenschein
author2	Sara Skøtt Paulsen Mikael Lenz Strube Pernille Kjersgaard Bech Lone Gram Eva C. Sonnenschein
format	Journal article
container_title	mSystems
container_volume	4
container_issue	4
publishDate	2019
institution	Swansea University
issn	2379-5077
doi_str_mv	10.1128/msystems.00060-19
publisher	American Society for Microbiology
college_str	Faculty of Science and Engineering
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hierarchy_top_title	Faculty of Science and Engineering
hierarchy_parent_id	facultyofscienceandengineering
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department_str	School of Biosciences, Geography and Physics - Biosciences{{{_:::_}}}Faculty of Science and Engineering{{{_:::_}}}School of Biosciences, Geography and Physics - Biosciences
document_store_str	1
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description	Chitin is the most abundant polymer in the marine environment and a nutrient-rich surface for adhering marine bacteria. We have previously shown that chitin can induce the production of antibiotic compounds in Vibrionaceae, suggesting that the discovery of novel bioactive molecules from bacteria can be facilitated by mimicking their natural habitat. The purpose of this study was to determine the glycosyl hydrolase (GH) profiles of strains of the genus Pseudoalteromonas to enable selection of presumed growth substrates and explore possible links to secondary metabolism. Genomic analyses were conducted on 62 pigmented and 95 nonpigmented strains. Analysis of the total GH profiles and multidimensional scaling suggested that the degradation of chitin is a significant trait of pigmented strains, whereas nonpigmented strains seem to be driven toward the degradation of alga-derived carbohydrates. The genomes of all pigmented strains and 40 nonpigmented strains encoded at least one conserved chitin degradation cluster, and chitinolytic activity was phenotypically confirmed. Additionally, the genomes of all pigmented and a few nonpigmented strains encoded chitinases of the rare GH family 19. Pigmented strains devote up to 15% of their genome to secondary metabolism, while for nonpigmented species it was 3% at most. Thus, pigmented Pseudoalteromonas strains have a bioactive potential similar to that of well-known antibiotic producers of the Actinobacteria phylum. Growth on chitin did not measurably enhance the antibacterial activity of the strains; however, we demonstrated a remarkable co-occurrence of chitin degradation and the potential for secondary metabolite production in pigmented Pseudoalteromonas strains. This indicates that chitin and its colonizers of the Pseudoalteromonas genus represent a so far underexplored niche for novel enzymes and bioactive compounds.
published_date	2019-08-27T04:20:46Z
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score	11.016235

Marine Chitinolytic Pseudoalteromonas Represents an Untapped Reservoir of Bioactive Potential

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