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Enzymes Encapsulated within Alginate Hydrogels: Bioelectrocatalysis and Electrochemiluminescence Applications

Simona Ferraraccio Orcid Logo, Donatella Di Lisa, Laura Pastorino Orcid Logo, Paolo Bertoncello Orcid Logo

Analytical Chemistry, Volume: 94, Issue: 46, Pages: 16122 - 16131

Swansea University Authors: Simona Ferraraccio Orcid Logo, Paolo Bertoncello Orcid Logo

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DOI (Published version): 10.1021/acs.analchem.2c03389

Abstract

A simple procedure to incorporate enzymes (horseradish peroxidase, HRP, and lactate oxidase, LOx) within alginate hydrogels is reported with electrochemiluminescence (ECL) used to detect the enzymatic reactions with the corresponding substrates. First, HRP and LOx were successfully immobilized into...

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Published in: Analytical Chemistry
ISSN: 0003-2700 1520-6882
Published: American Chemical Society (ACS) 2022
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URI: https://cronfa.swan.ac.uk/Record/cronfa61826
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First, HRP and LOx were successfully immobilized into CaCO3 microspheres, followed by the electrostatic layer-by-layer deposition of a nanoshell onto the microspheres, and finally by their dispersion into alginate solution. The as-prepared dispersion was drop cast onto the glassy carbon electrodes and cross-linked by the external and internal gelation methods using Ca2+ cations. The enzymes encapsulated within the alginate hydrogels were characterized using cyclic voltammetry and kinetic studies performed using ECL. The results showed that the enzymatic activity was significantly maintained as a result of the immobilization, with values of the apparent Michaelis&#x2013;Menten constants estimated as 7.71 &#xB1; 0.62 and 8.41 &#xB1; 0.43 &#x3BC;M, for HRP and LOx, respectively. The proposed biosensors showed good stability and repeatability with an estimated limit of detection of 5.38 &#xB1; 0.05 and 0.50 &#xB1; 0.03 &#x3BC;M for hydrogen peroxide and lactic acid, respectively. The as-prepared enzymes encapsulated within the alginate hydrogels showed good stability up to 28 days from their preparation. The sensitivity and selectivity of the enzymes encapsulated within the alginate hydrogels were tested in real matrices (HRP, hydrogen peroxide, in contact lens solution; LOx, lactic acid in artificial sweat) showing the sensitivity of the ECL detection methods for the detection of hydrogen peroxide and lactic acid in real samples.</abstract><type>Journal Article</type><journal>Analytical Chemistry</journal><volume>94</volume><journalNumber>46</journalNumber><paginationStart>16122</paginationStart><paginationEnd>16131</paginationEnd><publisher>American Chemical Society (ACS)</publisher><placeOfPublication/><isbnPrint/><isbnElectronic/><issnPrint>0003-2700</issnPrint><issnElectronic>1520-6882</issnElectronic><keywords/><publishedDay>22</publishedDay><publishedMonth>11</publishedMonth><publishedYear>2022</publishedYear><publishedDate>2022-11-22</publishedDate><doi>10.1021/acs.analchem.2c03389</doi><url/><notes/><college>COLLEGE NANME</college><department>Chemical Engineering</department><CollegeCode>COLLEGE CODE</CollegeCode><DepartmentCode>CHEG</DepartmentCode><institution>Swansea University</institution><apcterm>SU Library paid the OA fee (TA Institutional Deal)</apcterm><funders>L.S.F. gratefully acknowledges financial support from the Knowledge Economy Skills PhD Scholarship (KESS2) under the Welsh Government&#x2019;s European Social Fund (ESF) convergence program for West Wales and the Valleys and Perpetuus Carbon Ltd.</funders><projectreference/><lastEdited>2023-01-05T16:12:39.8372153</lastEdited><Created>2022-11-09T09:33:24.2408993</Created><path><level id="1">Faculty of Science and Engineering</level><level id="2">School of Engineering and Applied Sciences - Chemical Engineering</level></path><authors><author><firstname>Simona</firstname><surname>Ferraraccio</surname><orcid>0000-0001-6949-6882</orcid><order>1</order></author><author><firstname>Donatella Di</firstname><surname>Lisa</surname><order>2</order></author><author><firstname>Laura</firstname><surname>Pastorino</surname><orcid>0000-0002-5928-3856</orcid><order>3</order></author><author><firstname>Paolo</firstname><surname>Bertoncello</surname><orcid>0000-0002-6557-7885</orcid><order>4</order></author></authors><documents><document><filename>61826__25704__05fc816d00164ea4a92d6d95f4e7a9e4.pdf</filename><originalFilename>61826_VoR.pdf</originalFilename><uploaded>2022-11-09T10:04:38.0547047</uploaded><type>Output</type><contentLength>6761367</contentLength><contentType>application/pdf</contentType><version>Version of Record</version><cronfaStatus>true</cronfaStatus><documentNotes>Released under the terms of a Creative Commons Attribution License (CC-BY 4.0)</documentNotes><copyrightCorrect>true</copyrightCorrect><language>eng</language><licence>https://creativecommons.org/licenses/by/4.0/</licence></document></documents><OutputDurs/></rfc1807>
spelling 2023-01-05T16:12:39.8372153 v2 61826 2022-11-09 Enzymes Encapsulated within Alginate Hydrogels: Bioelectrocatalysis and Electrochemiluminescence Applications 1300f1211abc29e989f5aee998831070 0000-0001-6949-6882 Simona Ferraraccio Simona Ferraraccio true false ad352842aa5fe9c1947bd24ff61816c8 0000-0002-6557-7885 Paolo Bertoncello Paolo Bertoncello true false 2022-11-09 CHEG A simple procedure to incorporate enzymes (horseradish peroxidase, HRP, and lactate oxidase, LOx) within alginate hydrogels is reported with electrochemiluminescence (ECL) used to detect the enzymatic reactions with the corresponding substrates. First, HRP and LOx were successfully immobilized into CaCO3 microspheres, followed by the electrostatic layer-by-layer deposition of a nanoshell onto the microspheres, and finally by their dispersion into alginate solution. The as-prepared dispersion was drop cast onto the glassy carbon electrodes and cross-linked by the external and internal gelation methods using Ca2+ cations. The enzymes encapsulated within the alginate hydrogels were characterized using cyclic voltammetry and kinetic studies performed using ECL. The results showed that the enzymatic activity was significantly maintained as a result of the immobilization, with values of the apparent Michaelis–Menten constants estimated as 7.71 ± 0.62 and 8.41 ± 0.43 μM, for HRP and LOx, respectively. The proposed biosensors showed good stability and repeatability with an estimated limit of detection of 5.38 ± 0.05 and 0.50 ± 0.03 μM for hydrogen peroxide and lactic acid, respectively. The as-prepared enzymes encapsulated within the alginate hydrogels showed good stability up to 28 days from their preparation. The sensitivity and selectivity of the enzymes encapsulated within the alginate hydrogels were tested in real matrices (HRP, hydrogen peroxide, in contact lens solution; LOx, lactic acid in artificial sweat) showing the sensitivity of the ECL detection methods for the detection of hydrogen peroxide and lactic acid in real samples. Journal Article Analytical Chemistry 94 46 16122 16131 American Chemical Society (ACS) 0003-2700 1520-6882 22 11 2022 2022-11-22 10.1021/acs.analchem.2c03389 COLLEGE NANME Chemical Engineering COLLEGE CODE CHEG Swansea University SU Library paid the OA fee (TA Institutional Deal) L.S.F. gratefully acknowledges financial support from the Knowledge Economy Skills PhD Scholarship (KESS2) under the Welsh Government’s European Social Fund (ESF) convergence program for West Wales and the Valleys and Perpetuus Carbon Ltd. 2023-01-05T16:12:39.8372153 2022-11-09T09:33:24.2408993 Faculty of Science and Engineering School of Engineering and Applied Sciences - Chemical Engineering Simona Ferraraccio 0000-0001-6949-6882 1 Donatella Di Lisa 2 Laura Pastorino 0000-0002-5928-3856 3 Paolo Bertoncello 0000-0002-6557-7885 4 61826__25704__05fc816d00164ea4a92d6d95f4e7a9e4.pdf 61826_VoR.pdf 2022-11-09T10:04:38.0547047 Output 6761367 application/pdf Version of Record true Released under the terms of a Creative Commons Attribution License (CC-BY 4.0) true eng https://creativecommons.org/licenses/by/4.0/
title Enzymes Encapsulated within Alginate Hydrogels: Bioelectrocatalysis and Electrochemiluminescence Applications
spellingShingle Enzymes Encapsulated within Alginate Hydrogels: Bioelectrocatalysis and Electrochemiluminescence Applications
Simona Ferraraccio
Paolo Bertoncello
title_short Enzymes Encapsulated within Alginate Hydrogels: Bioelectrocatalysis and Electrochemiluminescence Applications
title_full Enzymes Encapsulated within Alginate Hydrogels: Bioelectrocatalysis and Electrochemiluminescence Applications
title_fullStr Enzymes Encapsulated within Alginate Hydrogels: Bioelectrocatalysis and Electrochemiluminescence Applications
title_full_unstemmed Enzymes Encapsulated within Alginate Hydrogels: Bioelectrocatalysis and Electrochemiluminescence Applications
title_sort Enzymes Encapsulated within Alginate Hydrogels: Bioelectrocatalysis and Electrochemiluminescence Applications
author_id_str_mv 1300f1211abc29e989f5aee998831070
ad352842aa5fe9c1947bd24ff61816c8
author_id_fullname_str_mv 1300f1211abc29e989f5aee998831070_***_Simona Ferraraccio
ad352842aa5fe9c1947bd24ff61816c8_***_Paolo Bertoncello
author Simona Ferraraccio
Paolo Bertoncello
author2 Simona Ferraraccio
Donatella Di Lisa
Laura Pastorino
Paolo Bertoncello
format Journal article
container_title Analytical Chemistry
container_volume 94
container_issue 46
container_start_page 16122
publishDate 2022
institution Swansea University
issn 0003-2700
1520-6882
doi_str_mv 10.1021/acs.analchem.2c03389
publisher American Chemical Society (ACS)
college_str Faculty of Science and Engineering
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hierarchy_top_id facultyofscienceandengineering
hierarchy_top_title Faculty of Science and Engineering
hierarchy_parent_id facultyofscienceandengineering
hierarchy_parent_title Faculty of Science and Engineering
department_str School of Engineering and Applied Sciences - Chemical Engineering{{{_:::_}}}Faculty of Science and Engineering{{{_:::_}}}School of Engineering and Applied Sciences - Chemical Engineering
document_store_str 1
active_str 0
description A simple procedure to incorporate enzymes (horseradish peroxidase, HRP, and lactate oxidase, LOx) within alginate hydrogels is reported with electrochemiluminescence (ECL) used to detect the enzymatic reactions with the corresponding substrates. First, HRP and LOx were successfully immobilized into CaCO3 microspheres, followed by the electrostatic layer-by-layer deposition of a nanoshell onto the microspheres, and finally by their dispersion into alginate solution. The as-prepared dispersion was drop cast onto the glassy carbon electrodes and cross-linked by the external and internal gelation methods using Ca2+ cations. The enzymes encapsulated within the alginate hydrogels were characterized using cyclic voltammetry and kinetic studies performed using ECL. The results showed that the enzymatic activity was significantly maintained as a result of the immobilization, with values of the apparent Michaelis–Menten constants estimated as 7.71 ± 0.62 and 8.41 ± 0.43 μM, for HRP and LOx, respectively. The proposed biosensors showed good stability and repeatability with an estimated limit of detection of 5.38 ± 0.05 and 0.50 ± 0.03 μM for hydrogen peroxide and lactic acid, respectively. The as-prepared enzymes encapsulated within the alginate hydrogels showed good stability up to 28 days from their preparation. The sensitivity and selectivity of the enzymes encapsulated within the alginate hydrogels were tested in real matrices (HRP, hydrogen peroxide, in contact lens solution; LOx, lactic acid in artificial sweat) showing the sensitivity of the ECL detection methods for the detection of hydrogen peroxide and lactic acid in real samples.
published_date 2022-11-22T04:20:57Z
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score 11.016235

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