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One-pot RAFT and fast polymersomes assembly: a ‘beeline’ from monomers to drug-loaded nanovectors
Polymer Chemistry, Volume: 7, Issue: 44, Pages: 6714 - 6724
Swansea University Author:
Georgina Marsh
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DOI (Published version): 10.1039/c6py01292b
Abstract
Rapid and simple routes to functional polymersomes are increasingly needed to expand their clinical or industrial applications. Here we describe a novel strategy where polymersomes are prepared through an in-line process in just a few hours, starting from simple acrylate or acrylamide monomers. Usin...
Published in: | Polymer Chemistry |
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ISSN: | 1759-9954 1759-9962 |
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Royal Society of Chemistry (RSC)
2016
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URI: | https://cronfa.swan.ac.uk/Record/cronfa64802 |
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v2 64802 2023-10-23 One-pot RAFT and fast polymersomes assembly: a ‘beeline’ from monomers to drug-loaded nanovectors 60eb67dc80ac6072cbea39caa88d662c 0000-0001-8621-2925 Georgina Marsh Georgina Marsh true false 2023-10-23 PHAR Rapid and simple routes to functional polymersomes are increasingly needed to expand their clinical or industrial applications. Here we describe a novel strategy where polymersomes are prepared through an in-line process in just a few hours, starting from simple acrylate or acrylamide monomers. Using Perrier's protocol, well-defined amphiphilic diblock copolymers formed from PEG acrylate (mPEGA480), 2-(acryloyloxy)ethyl-3-chloro-4-hydroxybenzoate (ACH) or 2-(3-chloro-4-hydroxybenzamido)ethyl acrylate (CHB), have been synthesised by RAFT polymerisation in one-pot, pushing the monomer conversion for each block close to completion (≥94%). The reaction mixture, consisting of green biocompatible solvents (ethanol/water) have then been directly utilised to generate well-defined polymersomes, by simple cannulation into water or in a more automated process, by using a bespoke microfluidic device. Terbinafine and cyanocobalamine were used to demonstrate the suitability of the process to incorporate model hydrophobic and hydrophilic drugs, respectively. Vesicles size and morphology were characterised by DLS, TEM, and AFM. In this work we show that materials and experimental conditions can be chosen to allow facile and rapid generation drug-loaded polymersomes, through a suitable in-line process, directly from acrylate or acrylamide monomer building blocks. Journal Article Polymer Chemistry 7 44 6714 6724 Royal Society of Chemistry (RSC) 1759-9954 1759-9962 Polymersomes, monomers 20 9 2016 2016-09-20 10.1039/c6py01292b http://dx.doi.org/10.1039/c6py01292b COLLEGE NANME Pharmacy COLLEGE CODE PHAR Swansea University The authors thank the UK Engineering and Physical Sciences Research Council (EPSRC: Grants EP/H006915/1 and EP/H005625/1 Leadership Fellowship (C. A.); EP/L01646X (A. F. B.); EP/I01375X/1 (G. E. M.)). 2023-11-15T14:29:41.8237061 2023-10-23T14:58:38.9174721 Faculty of Medicine, Health and Life Sciences Swansea University Medical School - Pharmacy F. Mastrotto 1 A. F. Breen 2 G. Sicilia 3 S. Murdan 4 A. D. Johnstone 5 Georgina Marsh 0000-0001-8621-2925 6 C. Grainger-Boultby 7 N. A. Russell 8 C. Alexander 0000-0001-8337-1875 9 G. Mantovani 10 64802__29032__2b2db73a09a6405d904b476ec3752b58.pdf 64802.VOR.pdf 2023-11-15T14:17:12.7484522 Output 3414221 application/pdf Version of Record true Distributed under the terms of a Creative Commons Attribution 3.0 Unported License (CC BY 3.0). true eng https://creativecommons.org/licenses/by/3.0/ |
title |
One-pot RAFT and fast polymersomes assembly: a ‘beeline’ from monomers to drug-loaded nanovectors |
spellingShingle |
One-pot RAFT and fast polymersomes assembly: a ‘beeline’ from monomers to drug-loaded nanovectors Georgina Marsh |
title_short |
One-pot RAFT and fast polymersomes assembly: a ‘beeline’ from monomers to drug-loaded nanovectors |
title_full |
One-pot RAFT and fast polymersomes assembly: a ‘beeline’ from monomers to drug-loaded nanovectors |
title_fullStr |
One-pot RAFT and fast polymersomes assembly: a ‘beeline’ from monomers to drug-loaded nanovectors |
title_full_unstemmed |
One-pot RAFT and fast polymersomes assembly: a ‘beeline’ from monomers to drug-loaded nanovectors |
title_sort |
One-pot RAFT and fast polymersomes assembly: a ‘beeline’ from monomers to drug-loaded nanovectors |
author_id_str_mv |
60eb67dc80ac6072cbea39caa88d662c |
author_id_fullname_str_mv |
60eb67dc80ac6072cbea39caa88d662c_***_Georgina Marsh |
author |
Georgina Marsh |
author2 |
F. Mastrotto A. F. Breen G. Sicilia S. Murdan A. D. Johnstone Georgina Marsh C. Grainger-Boultby N. A. Russell C. Alexander G. Mantovani |
format |
Journal article |
container_title |
Polymer Chemistry |
container_volume |
7 |
container_issue |
44 |
container_start_page |
6714 |
publishDate |
2016 |
institution |
Swansea University |
issn |
1759-9954 1759-9962 |
doi_str_mv |
10.1039/c6py01292b |
publisher |
Royal Society of Chemistry (RSC) |
college_str |
Faculty of Medicine, Health and Life Sciences |
hierarchytype |
|
hierarchy_top_id |
facultyofmedicinehealthandlifesciences |
hierarchy_top_title |
Faculty of Medicine, Health and Life Sciences |
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facultyofmedicinehealthandlifesciences |
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Faculty of Medicine, Health and Life Sciences |
department_str |
Swansea University Medical School - Pharmacy{{{_:::_}}}Faculty of Medicine, Health and Life Sciences{{{_:::_}}}Swansea University Medical School - Pharmacy |
url |
http://dx.doi.org/10.1039/c6py01292b |
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description |
Rapid and simple routes to functional polymersomes are increasingly needed to expand their clinical or industrial applications. Here we describe a novel strategy where polymersomes are prepared through an in-line process in just a few hours, starting from simple acrylate or acrylamide monomers. Using Perrier's protocol, well-defined amphiphilic diblock copolymers formed from PEG acrylate (mPEGA480), 2-(acryloyloxy)ethyl-3-chloro-4-hydroxybenzoate (ACH) or 2-(3-chloro-4-hydroxybenzamido)ethyl acrylate (CHB), have been synthesised by RAFT polymerisation in one-pot, pushing the monomer conversion for each block close to completion (≥94%). The reaction mixture, consisting of green biocompatible solvents (ethanol/water) have then been directly utilised to generate well-defined polymersomes, by simple cannulation into water or in a more automated process, by using a bespoke microfluidic device. Terbinafine and cyanocobalamine were used to demonstrate the suitability of the process to incorporate model hydrophobic and hydrophilic drugs, respectively. Vesicles size and morphology were characterised by DLS, TEM, and AFM. In this work we show that materials and experimental conditions can be chosen to allow facile and rapid generation drug-loaded polymersomes, through a suitable in-line process, directly from acrylate or acrylamide monomer building blocks. |
published_date |
2016-09-20T14:29:45Z |
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1782640631678500864 |
score |
11.024221 |