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In situ environmental conditions and molecular identification of the photosymbiotic marine worm Symsagittifera roscoffensis
Symbiosis, Volume: 92, Issue: 1, Pages: 137 - 148
Swansea University Authors:
NATHAN THOMAS, Kam Tang
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DOI (Published version): 10.1007/s13199-023-00964-2
Abstract
Known as the Roscoff worm or mint-sauce worm, Symsagittifera roscoffensis is an Acoel distinguishable due to the presence of symbiotic alga Tetraselmis convolutae, held beneath the epidermis. Isolated populations of S. roscoffensis span a broad geographical range along the north-eastern Atlantic coa...
Published in: | Symbiosis |
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ISSN: | 0334-5114 1878-7665 |
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Springer Science and Business Media LLC
2024
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URI: | https://cronfa.swan.ac.uk/Record/cronfa65190 |
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v2 65190 2023-12-03 In situ environmental conditions and molecular identification of the photosymbiotic marine worm Symsagittifera roscoffensis ea301a73007049cf191d0caf171a3b8c NATHAN THOMAS NATHAN THOMAS true false 69af43a3b9da24aef65c5d3a44956fe3 0000-0001-9427-9564 Kam Tang Kam Tang true false 2023-12-03 Known as the Roscoff worm or mint-sauce worm, Symsagittifera roscoffensis is an Acoel distinguishable due to the presence of symbiotic alga Tetraselmis convolutae, held beneath the epidermis. Isolated populations of S. roscoffensis span a broad geographical range along the north-eastern Atlantic coast, from Wales to Portugal. The only known population of the worm in the United Kingdom was discovered in Wales decades ago, but very little research has been done on it since. For 13 months, we measured how environmental conditions such as temperature, salinity and light intensity coincided with population size at the Welsh field site. To establish phylogenetic relationships among the different populations and their algal symbionts, we designed new polymerase chain reaction (PCR) oligonucleotides to assess the nucleotide diversity of the mitochondrial cytochrome c oxidase I subunit (COI) gene in gDNA extracted from representative worms across their known 25 range (Wales, France, Portugal, Spain, and Guernsey). We also targeted the 18S rRNA gene of their algal symbiont, Tetraselmis convolutae. We observed temporal shifts in environmental factors coinciding with fluctuating worm colony size, notably temperature. Based on the molecular data, the worm exhibited different ecotypes across locations, while the algal symbiont showed little genetic variation. Journal Article Symbiosis 92 1 137 148 Springer Science and Business Media LLC 0334-5114 1878-7665 Acoela; Tetraselmis spp.; marine phylogenetics; PCR; Symbiosis 1 1 2024 2024-01-01 10.1007/s13199-023-00964-2 http://dx.doi.org/10.1007/s13199-023-00964-2 COLLEGE NANME COLLEGE CODE Swansea University SU Library paid the OA fee (TA Institutional Deal) HEFCW & Swansea University RWIF (RIG1036-125) 2024-04-03T15:37:24.6848099 2023-12-03T13:29:20.4788327 Faculty of Science and Engineering School of Biosciences, Geography and Physics - Biosciences NATHAN THOMAS 1 Kam Tang 0000-0001-9427-9564 2 Christopher J. Coates 3 65190__29895__a82f5b279fc84fceb9ee73153b9ab7f9.pdf 65190.VOR.pdf 2024-04-03T15:35:57.5248777 Output 3159300 application/pdf Version of Record true This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence. true eng http://creativecommons.org/licenses/by/4.0/ |
title |
In situ environmental conditions and molecular identification of the photosymbiotic marine worm Symsagittifera roscoffensis |
spellingShingle |
In situ environmental conditions and molecular identification of the photosymbiotic marine worm Symsagittifera roscoffensis NATHAN THOMAS Kam Tang |
title_short |
In situ environmental conditions and molecular identification of the photosymbiotic marine worm Symsagittifera roscoffensis |
title_full |
In situ environmental conditions and molecular identification of the photosymbiotic marine worm Symsagittifera roscoffensis |
title_fullStr |
In situ environmental conditions and molecular identification of the photosymbiotic marine worm Symsagittifera roscoffensis |
title_full_unstemmed |
In situ environmental conditions and molecular identification of the photosymbiotic marine worm Symsagittifera roscoffensis |
title_sort |
In situ environmental conditions and molecular identification of the photosymbiotic marine worm Symsagittifera roscoffensis |
author_id_str_mv |
ea301a73007049cf191d0caf171a3b8c 69af43a3b9da24aef65c5d3a44956fe3 |
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ea301a73007049cf191d0caf171a3b8c_***_NATHAN THOMAS 69af43a3b9da24aef65c5d3a44956fe3_***_Kam Tang |
author |
NATHAN THOMAS Kam Tang |
author2 |
NATHAN THOMAS Kam Tang Christopher J. Coates |
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Symbiosis |
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92 |
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137 |
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2024 |
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Swansea University |
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0334-5114 1878-7665 |
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10.1007/s13199-023-00964-2 |
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Springer Science and Business Media LLC |
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Faculty of Science and Engineering |
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Faculty of Science and Engineering |
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http://dx.doi.org/10.1007/s13199-023-00964-2 |
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description |
Known as the Roscoff worm or mint-sauce worm, Symsagittifera roscoffensis is an Acoel distinguishable due to the presence of symbiotic alga Tetraselmis convolutae, held beneath the epidermis. Isolated populations of S. roscoffensis span a broad geographical range along the north-eastern Atlantic coast, from Wales to Portugal. The only known population of the worm in the United Kingdom was discovered in Wales decades ago, but very little research has been done on it since. For 13 months, we measured how environmental conditions such as temperature, salinity and light intensity coincided with population size at the Welsh field site. To establish phylogenetic relationships among the different populations and their algal symbionts, we designed new polymerase chain reaction (PCR) oligonucleotides to assess the nucleotide diversity of the mitochondrial cytochrome c oxidase I subunit (COI) gene in gDNA extracted from representative worms across their known 25 range (Wales, France, Portugal, Spain, and Guernsey). We also targeted the 18S rRNA gene of their algal symbiont, Tetraselmis convolutae. We observed temporal shifts in environmental factors coinciding with fluctuating worm colony size, notably temperature. Based on the molecular data, the worm exhibited different ecotypes across locations, while the algal symbiont showed little genetic variation. |
published_date |
2024-01-01T15:37:21Z |
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1795324684460359680 |
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11.017797 |