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Comparison of screening methods for high-throughput determination of oil yields in micro-algal biofuel strains

Steve Slocombe, QianYi Zhang, Kenneth D. Black, John G. Day, Michele S. Stanley

Journal of Applied Phycology, Volume: 25, Issue: 4, Pages: 961 - 972

Swansea University Author: Steve Slocombe

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Abstract

The phenotypic and phylogenetic diversity of micro-algae capable of accumulating triacylglycerols provides a challenge for the accurate determination of biotechnological potential. High-yielding strains are needed to improve economic viability and their compositional information is required for opti...

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Published in: Journal of Applied Phycology
ISSN: 0921-8971 1573-5176
Published: Springer Science and Business Media LLC 2013
Online Access: Check full text

URI: https://cronfa.swan.ac.uk/Record/cronfa65473
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Abstract: The phenotypic and phylogenetic diversity of micro-algae capable of accumulating triacylglycerols provides a challenge for the accurate determination of biotechnological potential. High-yielding strains are needed to improve economic viability and their compositional information is required for optimizing biodiesel properties. To facilitate a high-throughput screening programme, a very rapid direct-derivatization procedure capable of extracting lyophilized material for GC analysis was compared with a scaled-down Folch-based method. This was carried out on ten micro-algal strains from 6 phyla where the more rapid direct-derivatization approach was found to provide a more reliable measure of yield. The modified Folch-based procedure was found to substantially underestimate oil yield in one Chlorella species (P < 0.01). In terms of fatty acid composition however, the Folch procedure proved to be slightly better in recovering polyunsaturated fatty acids, in six out of the ten strains. Therefore, direct-derivatization is recommended for rapid determination of yields in screening approaches but can provide slightly less compositional accuracy than solvent-based extraction methods.
Keywords: Intelligent screening; In situ transesterification; Lipids; Biodiesel; Biofuel; Chlorella
College: Faculty of Science and Engineering
Issue: 4
Start Page: 961
End Page: 972