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Divergent Regulation of Terpenoid Metabolism in the Trichomes of Wild and Cultivated Tomato Species

Katrin Besser, Andrea Harper, Nicholas Welsby, Ines Schauvinhold, Steve Slocombe, Yi Li, Richard A. Dixon, Pierre Broun

Plant Physiology, Volume: 149, Issue: 1, Pages: 499 - 514

Swansea University Author: Steve Slocombe

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DOI (Published version): 10.1104/pp.108.126276

Abstract

The diversification of chemical production in glandular trichomes is important in the development of resistance against pathogens and pests in two species of tomato. We have used genetic and genomic approaches to uncover some of the biochemical and molecular mechanisms that underlie the divergence i...

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Published in: Plant Physiology
ISSN: 1532-2548
Published: Oxford University Press (OUP) 2009
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URI: https://cronfa.swan.ac.uk/Record/cronfa65490
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spelling v2 65490 2024-01-22 Divergent Regulation of Terpenoid Metabolism in the Trichomes of Wild and Cultivated Tomato Species 4a1ea486a78ed357efdfa053a277ae40 Steve Slocombe Steve Slocombe true false 2024-01-22 SBI The diversification of chemical production in glandular trichomes is important in the development of resistance against pathogens and pests in two species of tomato. We have used genetic and genomic approaches to uncover some of the biochemical and molecular mechanisms that underlie the divergence in trichome metabolism between the wild species Solanum habrochaites LA1777 and its cultivated relative, Solanum lycopersicum. LA1777 produces high amounts of insecticidal sesquiterpene carboxylic acids (SCAs), whereas cultivated tomatoes lack SCAs and are more susceptible to pests. We show that trichomes of the two species have nearly opposite terpenoid profiles, consisting mainly of monoterpenes and low levels of sesquiterpenes in S. lycopersicum and mainly of SCAs and very low monoterpene levels in LA1777. The accumulation patterns of these terpenoids are different during development, in contrast to the developmental expression profiles of terpenoid pathway genes, which are similar in the two species, but they do not correlate in either case with terpenoid accumulation. However, our data suggest that the accumulation of monoterpenes in S. lycopersicum and major sesquiterpenes in LA1777 are linked both genetically and biochemically. Metabolite analyses after targeted gene silencing, inhibitor treatments, and precursor feeding all show that sesquiterpene biosynthesis relies mainly on products from the plastidic 2-C-methyl-d-erythritol-4-phosphate pathway in LA1777 but less so in the cultivated species. Furthermore, two classes of sesquiterpenes produced by the wild species may be synthesized from distinct pools of precursors via cytosolic and plastidial cyclases. However, highly trichome-expressed sesquiterpene cyclase-like enzymes were ruled out as being involved in the production of major LA1777 sesquiterpenes. Journal Article Plant Physiology 149 1 499 514 Oxford University Press (OUP) 1532-2548 6 1 2009 2009-01-06 10.1104/pp.108.126276 COLLEGE NANME Biosciences COLLEGE CODE SBI Swansea University 2024-03-21T16:16:12.6868482 2024-01-22T14:44:08.0370607 Faculty of Science and Engineering School of Biosciences, Geography and Physics - Biosciences Katrin Besser 1 Andrea Harper 2 Nicholas Welsby 3 Ines Schauvinhold 4 Steve Slocombe 5 Yi Li 6 Richard A. Dixon 7 Pierre Broun 8
title Divergent Regulation of Terpenoid Metabolism in the Trichomes of Wild and Cultivated Tomato Species
spellingShingle Divergent Regulation of Terpenoid Metabolism in the Trichomes of Wild and Cultivated Tomato Species
Steve Slocombe
title_short Divergent Regulation of Terpenoid Metabolism in the Trichomes of Wild and Cultivated Tomato Species
title_full Divergent Regulation of Terpenoid Metabolism in the Trichomes of Wild and Cultivated Tomato Species
title_fullStr Divergent Regulation of Terpenoid Metabolism in the Trichomes of Wild and Cultivated Tomato Species
title_full_unstemmed Divergent Regulation of Terpenoid Metabolism in the Trichomes of Wild and Cultivated Tomato Species
title_sort Divergent Regulation of Terpenoid Metabolism in the Trichomes of Wild and Cultivated Tomato Species
author_id_str_mv 4a1ea486a78ed357efdfa053a277ae40
author_id_fullname_str_mv 4a1ea486a78ed357efdfa053a277ae40_***_Steve Slocombe
author Steve Slocombe
author2 Katrin Besser
Andrea Harper
Nicholas Welsby
Ines Schauvinhold
Steve Slocombe
Yi Li
Richard A. Dixon
Pierre Broun
format Journal article
container_title Plant Physiology
container_volume 149
container_issue 1
container_start_page 499
publishDate 2009
institution Swansea University
issn 1532-2548
doi_str_mv 10.1104/pp.108.126276
publisher Oxford University Press (OUP)
college_str Faculty of Science and Engineering
hierarchytype
hierarchy_top_id facultyofscienceandengineering
hierarchy_top_title Faculty of Science and Engineering
hierarchy_parent_id facultyofscienceandengineering
hierarchy_parent_title Faculty of Science and Engineering
department_str School of Biosciences, Geography and Physics - Biosciences{{{_:::_}}}Faculty of Science and Engineering{{{_:::_}}}School of Biosciences, Geography and Physics - Biosciences
document_store_str 0
active_str 0
description The diversification of chemical production in glandular trichomes is important in the development of resistance against pathogens and pests in two species of tomato. We have used genetic and genomic approaches to uncover some of the biochemical and molecular mechanisms that underlie the divergence in trichome metabolism between the wild species Solanum habrochaites LA1777 and its cultivated relative, Solanum lycopersicum. LA1777 produces high amounts of insecticidal sesquiterpene carboxylic acids (SCAs), whereas cultivated tomatoes lack SCAs and are more susceptible to pests. We show that trichomes of the two species have nearly opposite terpenoid profiles, consisting mainly of monoterpenes and low levels of sesquiterpenes in S. lycopersicum and mainly of SCAs and very low monoterpene levels in LA1777. The accumulation patterns of these terpenoids are different during development, in contrast to the developmental expression profiles of terpenoid pathway genes, which are similar in the two species, but they do not correlate in either case with terpenoid accumulation. However, our data suggest that the accumulation of monoterpenes in S. lycopersicum and major sesquiterpenes in LA1777 are linked both genetically and biochemically. Metabolite analyses after targeted gene silencing, inhibitor treatments, and precursor feeding all show that sesquiterpene biosynthesis relies mainly on products from the plastidic 2-C-methyl-d-erythritol-4-phosphate pathway in LA1777 but less so in the cultivated species. Furthermore, two classes of sesquiterpenes produced by the wild species may be synthesized from distinct pools of precursors via cytosolic and plastidial cyclases. However, highly trichome-expressed sesquiterpene cyclase-like enzymes were ruled out as being involved in the production of major LA1777 sesquiterpenes.
published_date 2009-01-06T16:16:13Z
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