Challenges to Implementing Environmental-DNA Monitoring in Namibia

Perry, Iain; Jâms, Ifan B.; Casas-Mulet, Roser; Hamutoko, Josefina; Marchbank, Angela; Lendelvo, Selma; Naomab, Erold; Mapani, Benjamin; Creer, Simon; Wanke, Heike; Durance, Isabelle; Kille, Peter

doi:10.3389/fenvs.2021.773991

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Challenges to Implementing Environmental-DNA Monitoring in Namibia

Iain Perry

, Ifan B. Jâms, Roser Casas-Mulet, Josefina Hamutoko, Angela Marchbank, Selma Lendelvo, Erold Naomab, Benjamin Mapani, Simon Creer, Heike Wanke, Isabelle Durance, Peter Kille

Frontiers in Environmental Science, Volume: 9

Swansea University Author: Iain Perry

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Copyright © 2022 Perry, Jâms, Casas-Mulet, Hamutoko, Marchbank, Lendelvo, Naomab, Mapani, Creer, Wanke, Durance and Kille. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY).
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DOI (Published version): 10.3389/fenvs.2021.773991

Abstract

By identifying fragments of DNA in the environment, eDNA approaches present apromising tool for monitoring biodiversity in a cost-effective way. This is particularlypertinent for countries where traditional morphological monitoring has been sparse.The first step to realising the potential of eDNA is...

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Published in:	Frontiers in Environmental Science
ISSN:	2296-665X
Published:	Frontiers Media SA 2022
Online Access:	Check full text
URI:	https://cronfa.swan.ac.uk/Record/cronfa65668
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spelling	v2 65668 2024-02-22 Challenges to Implementing Environmental-DNA Monitoring in Namibia 7d630cc1fa34fdcd873711c80a874322 0000-0001-8530-4086 Iain Perry Iain Perry true false 2024-02-22 BMS By identifying fragments of DNA in the environment, eDNA approaches present apromising tool for monitoring biodiversity in a cost-effective way. This is particularlypertinent for countries where traditional morphological monitoring has been sparse.The first step to realising the potential of eDNA is to develop methodologies that areadapted to local conditions. Here, we test field and laboratory eDNA protocols (aqueousand sediment samples) in a range of semi-arid ecosystems in Namibia. We successfullygathered eDNA data on a broad suite of organisms at multiple trophic levels (includingalgae, invertebrates and bacteria) but identified two key challenges to the implementationof eDNA methods in the region: 1) high turbidity requires a tailored sampling technique and2) identification of taxa by eDNA methods is currently constrained by a lack of referencedata. We hope this work will guide the deployment of eDNA biomonitoring in the aridecosystems of Namibia and neighbouring countries. Journal Article Frontiers in Environmental Science 9 Frontiers Media SA 2296-665X eDNA, Namibia, freshwater, biomonitoring, water security 17 1 2022 2022-01-17 10.3389/fenvs.2021.773991 COLLEGE NANME Biomedical Sciences COLLEGE CODE BMS Swansea University This work was funded by the Global Challenges Research Fund (GCRF) project HEFCW GCRF Small Project: Freshwater ecosystems, biodiversity and pathogens inNamibia awarded to Durance, Kille and Mapani. The data collected in December 2017 was done via an EPSRC Global Challenges Research Fund Institutional Sponsorship awarded to Casas-Mulet. 2024-03-26T12:47:33.6695600 2024-02-22T14:53:10.2797108 Faculty of Medicine, Health and Life Sciences Swansea University Medical School - Biomedical Science Iain Perry 0000-0001-8530-4086 1 Ifan B. Jâms 2 Roser Casas-Mulet 3 Josefina Hamutoko 4 Angela Marchbank 5 Selma Lendelvo 6 Erold Naomab 7 Benjamin Mapani 8 Simon Creer 9 Heike Wanke 10 Isabelle Durance 11 Peter Kille 12 65668__29853__1afd5ba04d004631b383eb54a6177086.pdf 65668.VOR.pdf 2024-03-26T12:45:23.6030286 Output 2257061 application/pdf Version of Record true Copyright © 2022 Perry, Jâms, Casas-Mulet, Hamutoko, Marchbank, Lendelvo, Naomab, Mapani, Creer, Wanke, Durance and Kille. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). true eng https://creativecommons.org/licenses/by/4.0/
title	Challenges to Implementing Environmental-DNA Monitoring in Namibia
spellingShingle	Challenges to Implementing Environmental-DNA Monitoring in Namibia Iain Perry
title_short	Challenges to Implementing Environmental-DNA Monitoring in Namibia
title_full	Challenges to Implementing Environmental-DNA Monitoring in Namibia
title_fullStr	Challenges to Implementing Environmental-DNA Monitoring in Namibia
title_full_unstemmed	Challenges to Implementing Environmental-DNA Monitoring in Namibia
title_sort	Challenges to Implementing Environmental-DNA Monitoring in Namibia
author_id_str_mv	7d630cc1fa34fdcd873711c80a874322
author_id_fullname_str_mv	7d630cc1fa34fdcd873711c80a874322_***_Iain Perry
author	Iain Perry
author2	Iain Perry Ifan B. Jâms Roser Casas-Mulet Josefina Hamutoko Angela Marchbank Selma Lendelvo Erold Naomab Benjamin Mapani Simon Creer Heike Wanke Isabelle Durance Peter Kille
format	Journal article
container_title	Frontiers in Environmental Science
container_volume	9
publishDate	2022
institution	Swansea University
issn	2296-665X
doi_str_mv	10.3389/fenvs.2021.773991
publisher	Frontiers Media SA
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description	By identifying fragments of DNA in the environment, eDNA approaches present apromising tool for monitoring biodiversity in a cost-effective way. This is particularlypertinent for countries where traditional morphological monitoring has been sparse.The first step to realising the potential of eDNA is to develop methodologies that areadapted to local conditions. Here, we test field and laboratory eDNA protocols (aqueousand sediment samples) in a range of semi-arid ecosystems in Namibia. We successfullygathered eDNA data on a broad suite of organisms at multiple trophic levels (includingalgae, invertebrates and bacteria) but identified two key challenges to the implementationof eDNA methods in the region: 1) high turbidity requires a tailored sampling technique and2) identification of taxa by eDNA methods is currently constrained by a lack of referencedata. We hope this work will guide the deployment of eDNA biomonitoring in the aridecosystems of Namibia and neighbouring countries.
published_date	2022-01-17T12:47:30Z
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Challenges to Implementing Environmental-DNA Monitoring in Namibia

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