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Challenges to Implementing Environmental-DNA Monitoring in Namibia
Frontiers in Environmental Science, Volume: 9
Swansea University Author:
Iain Perry
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Copyright © 2022 Perry, Jâms, Casas-Mulet, Hamutoko, Marchbank, Lendelvo, Naomab, Mapani, Creer, Wanke, Durance and Kille. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY).
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DOI (Published version): 10.3389/fenvs.2021.773991
Abstract
By identifying fragments of DNA in the environment, eDNA approaches present apromising tool for monitoring biodiversity in a cost-effective way. This is particularlypertinent for countries where traditional morphological monitoring has been sparse.The first step to realising the potential of eDNA is...
Published in: | Frontiers in Environmental Science |
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ISSN: | 2296-665X |
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2022
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URI: | https://cronfa.swan.ac.uk/Record/cronfa65668 |
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v2 65668 2024-02-22 Challenges to Implementing Environmental-DNA Monitoring in Namibia 7d630cc1fa34fdcd873711c80a874322 0000-0001-8530-4086 Iain Perry Iain Perry true false 2024-02-22 BMS By identifying fragments of DNA in the environment, eDNA approaches present apromising tool for monitoring biodiversity in a cost-effective way. This is particularlypertinent for countries where traditional morphological monitoring has been sparse.The first step to realising the potential of eDNA is to develop methodologies that areadapted to local conditions. Here, we test field and laboratory eDNA protocols (aqueousand sediment samples) in a range of semi-arid ecosystems in Namibia. We successfullygathered eDNA data on a broad suite of organisms at multiple trophic levels (includingalgae, invertebrates and bacteria) but identified two key challenges to the implementationof eDNA methods in the region: 1) high turbidity requires a tailored sampling technique and2) identification of taxa by eDNA methods is currently constrained by a lack of referencedata. We hope this work will guide the deployment of eDNA biomonitoring in the aridecosystems of Namibia and neighbouring countries. Journal Article Frontiers in Environmental Science 9 Frontiers Media SA 2296-665X eDNA, Namibia, freshwater, biomonitoring, water security 17 1 2022 2022-01-17 10.3389/fenvs.2021.773991 COLLEGE NANME Biomedical Sciences COLLEGE CODE BMS Swansea University This work was funded by the Global Challenges Research Fund (GCRF) project HEFCW GCRF Small Project: Freshwater ecosystems, biodiversity and pathogens inNamibia awarded to Durance, Kille and Mapani. The data collected in December 2017 was done via an EPSRC Global Challenges Research Fund Institutional Sponsorship awarded to Casas-Mulet. 2024-03-26T12:47:33.6695600 2024-02-22T14:53:10.2797108 Faculty of Medicine, Health and Life Sciences Swansea University Medical School - Biomedical Science Iain Perry 0000-0001-8530-4086 1 Ifan B. Jâms 2 Roser Casas-Mulet 3 Josefina Hamutoko 4 Angela Marchbank 5 Selma Lendelvo 6 Erold Naomab 7 Benjamin Mapani 8 Simon Creer 9 Heike Wanke 10 Isabelle Durance 11 Peter Kille 12 65668__29853__1afd5ba04d004631b383eb54a6177086.pdf 65668.VOR.pdf 2024-03-26T12:45:23.6030286 Output 2257061 application/pdf Version of Record true Copyright © 2022 Perry, Jâms, Casas-Mulet, Hamutoko, Marchbank, Lendelvo, Naomab, Mapani, Creer, Wanke, Durance and Kille. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). true eng https://creativecommons.org/licenses/by/4.0/ |
title |
Challenges to Implementing Environmental-DNA Monitoring in Namibia |
spellingShingle |
Challenges to Implementing Environmental-DNA Monitoring in Namibia Iain Perry |
title_short |
Challenges to Implementing Environmental-DNA Monitoring in Namibia |
title_full |
Challenges to Implementing Environmental-DNA Monitoring in Namibia |
title_fullStr |
Challenges to Implementing Environmental-DNA Monitoring in Namibia |
title_full_unstemmed |
Challenges to Implementing Environmental-DNA Monitoring in Namibia |
title_sort |
Challenges to Implementing Environmental-DNA Monitoring in Namibia |
author_id_str_mv |
7d630cc1fa34fdcd873711c80a874322 |
author_id_fullname_str_mv |
7d630cc1fa34fdcd873711c80a874322_***_Iain Perry |
author |
Iain Perry |
author2 |
Iain Perry Ifan B. Jâms Roser Casas-Mulet Josefina Hamutoko Angela Marchbank Selma Lendelvo Erold Naomab Benjamin Mapani Simon Creer Heike Wanke Isabelle Durance Peter Kille |
format |
Journal article |
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Frontiers in Environmental Science |
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9 |
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2022 |
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Swansea University |
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2296-665X |
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10.3389/fenvs.2021.773991 |
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Frontiers Media SA |
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Faculty of Medicine, Health and Life Sciences |
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Swansea University Medical School - Biomedical Science{{{_:::_}}}Faculty of Medicine, Health and Life Sciences{{{_:::_}}}Swansea University Medical School - Biomedical Science |
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description |
By identifying fragments of DNA in the environment, eDNA approaches present apromising tool for monitoring biodiversity in a cost-effective way. This is particularlypertinent for countries where traditional morphological monitoring has been sparse.The first step to realising the potential of eDNA is to develop methodologies that areadapted to local conditions. Here, we test field and laboratory eDNA protocols (aqueousand sediment samples) in a range of semi-arid ecosystems in Namibia. We successfullygathered eDNA data on a broad suite of organisms at multiple trophic levels (includingalgae, invertebrates and bacteria) but identified two key challenges to the implementationof eDNA methods in the region: 1) high turbidity requires a tailored sampling technique and2) identification of taxa by eDNA methods is currently constrained by a lack of referencedata. We hope this work will guide the deployment of eDNA biomonitoring in the aridecosystems of Namibia and neighbouring countries. |
published_date |
2022-01-17T12:47:30Z |
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