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A cyst-forming coccidian with large geographical range infecting forest and commensal rodents: Sarcocystis muricoelognathis sp. nov.

Tao Qin, Paula Ortega-Perez, Gudrun Wibbelt, Maklarin B. Lakim, Sulaiman Ginting, Yuvaluk Khoprasert, Konstans Wells Orcid Logo, Junjie Hu, Thomas Jäkel

Parasites and Vectors, Volume: 17, Issue: 1

Swansea University Author: Konstans Wells Orcid Logo

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DOI (Published version): 10.1186/s13071-024-06230-8

Abstract

The geographic distribution and host-parasite interaction networks of Sarcocystis spp. in small mammals in eastern Asia remain incompletely known.Experimental infections, morphological and molecular characterizations were used for discrimination of a new Sarcocystis species isolated from colubrid sn...

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Published in: Parasites and Vectors
ISSN: 1756-3305
Published: Springer Science and Business Media LLC 2024
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Sarcocystis sporocysts collected from rat snakes (Coelognathus radiatus, C. flavolineatus) in Thailand induced development of sarcocysts in experimental SD rats showing a type 10a cyst wall ultrastructure that was identical with those found in Rattus norvegicus from China and the forest rat Maxomys whiteheadi in Borneo. Its cystozoites had equal sizes in all intermediate hosts and locations, while sporocysts and cystozoites were distinct from other Sarcocystis species. Partial 28S rRNA sequences of S. muricoelognathis from M. whiteheadi were largely identical to those from R. norvegicus in China but distinct from newly sequenced Sarcocystis zuoi. The phylogeny of the nuclear 18S rRNA gene placed S. muricoelognathis within the so-called S. zuoi complex, including Sarcocystis attenuati, S. kani, S. scandentiborneensis and S. zuoi, while the latter clustered with the new species. However, the phylogeny of the ITS1-region confirmed the distinction between S. muricoelognathis and S. zuoi. Moreover, all three gene trees suggested that an isolate previously addressed as S. zuoi from Thailand (KU341120) is conspecific with S. muricoelognathis. Partial mitochondrial cox1 sequences of S. muricoelognathis were almost identical with those from other members of the group suggesting a shared, recent ancestry. Additionally, we isolated two partial 28S rRNA Sarcocystis sequences from Low’s squirrel Sundasciurus lowii that clustered with those of S. scandentiborneensis from treeshews.Our results provide strong evidence of broad geographic distributions of rodent-associated Sarcocystis and host shifts between commensal and forest small mammal species, even if the known host associations remain likely only snapshots of the true associations.</abstract><type>Journal Article</type><journal>Parasites and Vectors</journal><volume>17</volume><journalNumber>1</journalNumber><paginationStart/><paginationEnd/><publisher>Springer Science and Business Media LLC</publisher><placeOfPublication/><isbnPrint/><isbnElectronic/><issnPrint/><issnElectronic>1756-3305</issnElectronic><keywords>Rattus norvegicus, Maxomys whiteheadi, Coelognathus radiatus, Coelognathus favolineatus, Sarcocystis muricoelognathis, Life cycle, Morphological and molecular characterization, Sarcocystis zuoi-complex</keywords><publishedDay>15</publishedDay><publishedMonth>3</publishedMonth><publishedYear>2024</publishedYear><publishedDate>2024-03-15</publishedDate><doi>10.1186/s13071-024-06230-8</doi><url/><notes/><college>COLLEGE NANME</college><department>Biosciences</department><CollegeCode>COLLEGE CODE</CollegeCode><DepartmentCode>SBI</DepartmentCode><institution>Swansea University</institution><apcterm>Another institution paid the OA fee</apcterm><funders>German Federal Ministry of Economic Cooperation and Development (BMZ) (Project No. 2002.2156.4), the Natural Sciences Foundation of China (Grant 32260119)</funders><projectreference/><lastEdited>2024-04-17T17:06:38.1495376</lastEdited><Created>2024-03-16T21:22:28.6283175</Created><path><level id="1">Faculty of Science and Engineering</level><level id="2">School of Biosciences, Geography and Physics - Biosciences</level></path><authors><author><firstname>Tao</firstname><surname>Qin</surname><order>1</order></author><author><firstname>Paula</firstname><surname>Ortega-Perez</surname><order>2</order></author><author><firstname>Gudrun</firstname><surname>Wibbelt</surname><order>3</order></author><author><firstname>Maklarin B.</firstname><surname>Lakim</surname><order>4</order></author><author><firstname>Sulaiman</firstname><surname>Ginting</surname><order>5</order></author><author><firstname>Yuvaluk</firstname><surname>Khoprasert</surname><order>6</order></author><author><firstname>Konstans</firstname><surname>Wells</surname><orcid>0000-0003-0377-2463</orcid><order>7</order></author><author><firstname>Junjie</firstname><surname>Hu</surname><order>8</order></author><author><firstname>Thomas</firstname><surname>Jäkel</surname><order>9</order></author></authors><documents><document><filename>65850__29731__17fc20684e174a8a81eb4f56e62d7bf4.pdf</filename><originalFilename>Qin_etal_2024_ParasitesVectors.pdf</originalFilename><uploaded>2024-03-16T21:27:35.5825821</uploaded><type>Output</type><contentLength>3109271</contentLength><contentType>application/pdf</contentType><version>Version of Record</version><cronfaStatus>true</cronfaStatus><documentNotes>© The Author(s) 2024. 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spelling v2 65850 2024-03-16 A cyst-forming coccidian with large geographical range infecting forest and commensal rodents: Sarcocystis muricoelognathis sp. nov. d18166c31e89833c55ef0f2cbb551243 0000-0003-0377-2463 Konstans Wells Konstans Wells true false 2024-03-16 SBI The geographic distribution and host-parasite interaction networks of Sarcocystis spp. in small mammals in eastern Asia remain incompletely known.Experimental infections, morphological and molecular characterizations were used for discrimination of a new Sarcocystis species isolated from colubrid snakes and small mammals collected in Thailand, Borneo and China.We identified a new species, Sarcocystis muricoelognathis sp. nov., that features a relatively wide geographic distribution and infects both commensal and forest-inhabiting intermediate hosts. Sarcocystis sporocysts collected from rat snakes (Coelognathus radiatus, C. flavolineatus) in Thailand induced development of sarcocysts in experimental SD rats showing a type 10a cyst wall ultrastructure that was identical with those found in Rattus norvegicus from China and the forest rat Maxomys whiteheadi in Borneo. Its cystozoites had equal sizes in all intermediate hosts and locations, while sporocysts and cystozoites were distinct from other Sarcocystis species. Partial 28S rRNA sequences of S. muricoelognathis from M. whiteheadi were largely identical to those from R. norvegicus in China but distinct from newly sequenced Sarcocystis zuoi. The phylogeny of the nuclear 18S rRNA gene placed S. muricoelognathis within the so-called S. zuoi complex, including Sarcocystis attenuati, S. kani, S. scandentiborneensis and S. zuoi, while the latter clustered with the new species. However, the phylogeny of the ITS1-region confirmed the distinction between S. muricoelognathis and S. zuoi. Moreover, all three gene trees suggested that an isolate previously addressed as S. zuoi from Thailand (KU341120) is conspecific with S. muricoelognathis. Partial mitochondrial cox1 sequences of S. muricoelognathis were almost identical with those from other members of the group suggesting a shared, recent ancestry. Additionally, we isolated two partial 28S rRNA Sarcocystis sequences from Low’s squirrel Sundasciurus lowii that clustered with those of S. scandentiborneensis from treeshews.Our results provide strong evidence of broad geographic distributions of rodent-associated Sarcocystis and host shifts between commensal and forest small mammal species, even if the known host associations remain likely only snapshots of the true associations. Journal Article Parasites and Vectors 17 1 Springer Science and Business Media LLC 1756-3305 Rattus norvegicus, Maxomys whiteheadi, Coelognathus radiatus, Coelognathus favolineatus, Sarcocystis muricoelognathis, Life cycle, Morphological and molecular characterization, Sarcocystis zuoi-complex 15 3 2024 2024-03-15 10.1186/s13071-024-06230-8 COLLEGE NANME Biosciences COLLEGE CODE SBI Swansea University Another institution paid the OA fee German Federal Ministry of Economic Cooperation and Development (BMZ) (Project No. 2002.2156.4), the Natural Sciences Foundation of China (Grant 32260119) 2024-04-17T17:06:38.1495376 2024-03-16T21:22:28.6283175 Faculty of Science and Engineering School of Biosciences, Geography and Physics - Biosciences Tao Qin 1 Paula Ortega-Perez 2 Gudrun Wibbelt 3 Maklarin B. Lakim 4 Sulaiman Ginting 5 Yuvaluk Khoprasert 6 Konstans Wells 0000-0003-0377-2463 7 Junjie Hu 8 Thomas Jäkel 9 65850__29731__17fc20684e174a8a81eb4f56e62d7bf4.pdf Qin_etal_2024_ParasitesVectors.pdf 2024-03-16T21:27:35.5825821 Output 3109271 application/pdf Version of Record true © The Author(s) 2024. This article is licensed under a Creative Commons Attribution 4.0 International License. true eng http://creativecommons.org/licenses/by/4.0/
title A cyst-forming coccidian with large geographical range infecting forest and commensal rodents: Sarcocystis muricoelognathis sp. nov.
spellingShingle A cyst-forming coccidian with large geographical range infecting forest and commensal rodents: Sarcocystis muricoelognathis sp. nov.
Konstans Wells
title_short A cyst-forming coccidian with large geographical range infecting forest and commensal rodents: Sarcocystis muricoelognathis sp. nov.
title_full A cyst-forming coccidian with large geographical range infecting forest and commensal rodents: Sarcocystis muricoelognathis sp. nov.
title_fullStr A cyst-forming coccidian with large geographical range infecting forest and commensal rodents: Sarcocystis muricoelognathis sp. nov.
title_full_unstemmed A cyst-forming coccidian with large geographical range infecting forest and commensal rodents: Sarcocystis muricoelognathis sp. nov.
title_sort A cyst-forming coccidian with large geographical range infecting forest and commensal rodents: Sarcocystis muricoelognathis sp. nov.
author_id_str_mv d18166c31e89833c55ef0f2cbb551243
author_id_fullname_str_mv d18166c31e89833c55ef0f2cbb551243_***_Konstans Wells
author Konstans Wells
author2 Tao Qin
Paula Ortega-Perez
Gudrun Wibbelt
Maklarin B. Lakim
Sulaiman Ginting
Yuvaluk Khoprasert
Konstans Wells
Junjie Hu
Thomas Jäkel
format Journal article
container_title Parasites and Vectors
container_volume 17
container_issue 1
publishDate 2024
institution Swansea University
issn 1756-3305
doi_str_mv 10.1186/s13071-024-06230-8
publisher Springer Science and Business Media LLC
college_str Faculty of Science and Engineering
hierarchytype
hierarchy_top_id facultyofscienceandengineering
hierarchy_top_title Faculty of Science and Engineering
hierarchy_parent_id facultyofscienceandengineering
hierarchy_parent_title Faculty of Science and Engineering
department_str School of Biosciences, Geography and Physics - Biosciences{{{_:::_}}}Faculty of Science and Engineering{{{_:::_}}}School of Biosciences, Geography and Physics - Biosciences
document_store_str 1
active_str 0
description The geographic distribution and host-parasite interaction networks of Sarcocystis spp. in small mammals in eastern Asia remain incompletely known.Experimental infections, morphological and molecular characterizations were used for discrimination of a new Sarcocystis species isolated from colubrid snakes and small mammals collected in Thailand, Borneo and China.We identified a new species, Sarcocystis muricoelognathis sp. nov., that features a relatively wide geographic distribution and infects both commensal and forest-inhabiting intermediate hosts. Sarcocystis sporocysts collected from rat snakes (Coelognathus radiatus, C. flavolineatus) in Thailand induced development of sarcocysts in experimental SD rats showing a type 10a cyst wall ultrastructure that was identical with those found in Rattus norvegicus from China and the forest rat Maxomys whiteheadi in Borneo. Its cystozoites had equal sizes in all intermediate hosts and locations, while sporocysts and cystozoites were distinct from other Sarcocystis species. Partial 28S rRNA sequences of S. muricoelognathis from M. whiteheadi were largely identical to those from R. norvegicus in China but distinct from newly sequenced Sarcocystis zuoi. The phylogeny of the nuclear 18S rRNA gene placed S. muricoelognathis within the so-called S. zuoi complex, including Sarcocystis attenuati, S. kani, S. scandentiborneensis and S. zuoi, while the latter clustered with the new species. However, the phylogeny of the ITS1-region confirmed the distinction between S. muricoelognathis and S. zuoi. Moreover, all three gene trees suggested that an isolate previously addressed as S. zuoi from Thailand (KU341120) is conspecific with S. muricoelognathis. Partial mitochondrial cox1 sequences of S. muricoelognathis were almost identical with those from other members of the group suggesting a shared, recent ancestry. Additionally, we isolated two partial 28S rRNA Sarcocystis sequences from Low’s squirrel Sundasciurus lowii that clustered with those of S. scandentiborneensis from treeshews.Our results provide strong evidence of broad geographic distributions of rodent-associated Sarcocystis and host shifts between commensal and forest small mammal species, even if the known host associations remain likely only snapshots of the true associations.
published_date 2024-03-15T17:06:33Z
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