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Chromosome‐level reference genome and resequencing of 322 accessions reveal evolution, genomic imprint and key agronomic traits in adzuki bean
,
Kai Yang,
Chunhai Chen,
Bo Zhao,
Yanan Hou,
Wanqiu Wang,
Pu Zhao,
Kaili Wang,
Binhu Wang,
Ying Xiao,
Yongqiang Li,
Yisong Li,
Qijian Song,
Biao Liu,
Ruoxi Fan,
Abhishek Bohra,
Jianping Yu,
Eva C. Sonnenschein ,
Rajeev K Varshney ,
Zhixi Tian ,
Jianbo Jian,
Ping Wan
Plant Biotechnology Journal, Volume: 22, Issue: 8, Pages: 2173 - 2185
Swansea University Author:
Eva C. Sonnenschein
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DOI (Published version): 10.1111/pbi.14337
Abstract
Adzuki bean (Vigna angularis) is an important legume crop cultivated in over 30 countries worldwide. We developed a high-quality chromosome-level reference genome of adzuki bean cultivar Jingnong6 by combining PacBio Sequel long-read sequencing with short-read and Hi-C technologies. The assembled ge...
| Published in: | Plant Biotechnology Journal |
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| ISSN: | 1467-7644 1467-7652 |
| Published: |
Wiley
2024
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| Online Access: |
Check full text
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| URI: | https://cronfa.swan.ac.uk/Record/cronfa66578 |
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| Abstract: |
Adzuki bean (Vigna angularis) is an important legume crop cultivated in over 30 countries worldwide. We developed a high-quality chromosome-level reference genome of adzuki bean cultivar Jingnong6 by combining PacBio Sequel long-read sequencing with short-read and Hi-C technologies. The assembled genome covers 97.8% of the adzuki bean genome with a contig N50 of approximately 16 Mb and a total of 32 738 protein-coding genes. We also generated a comprehensive genome variation map of adzuki bean by whole-genome resequencing (WGRS) of 322 diverse adzuki beans accessions including both wild and cultivated. Furthermore, we have conducted comparative genomics and a genome-wide association study (GWAS) on key agricultural traits to investigate the evolution and domestication. GWAS identified several candidate genes, including VaCycA3;1, VaHB15, VaANR1 and VaBm, that exhibited significant associations with domestication traits. Furthermore, we conducted functional analyses on the roles of VaANR1 and VaBm in regulating seed coat colour. We provided evidence for the highest genetic diversity of wild adzuki (Vigna angularis var. nipponensis) in China with the presence of the most original wild adzuki bean, and the occurrence of domestication process facilitating transition from wild to cultigen. The present study elucidates the genetic basis of adzuki bean domestication traits and provides crucial genomic resources to support future breeding efforts in adzuki bean. |
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| Keywords: |
Adzuki bean, domestication, GWAS, agronomictraits, seed coat colour |
| College: |
Faculty of Science and Engineering |
| Funders: |
Beijing Municipal Natural Science Foundation. Grant Number: KZ201710020013
National Natural Science Foundation of China. Grant Number: 31871697
National Key Research and Development Program of China. Grant Numbers: 2018YFD1000705, 2018YFD1000700
Food Futures Institute (Murdoch University)
New varieties breeding and Experimental demonstration of adzuki bean. Grant Number: 2024115272-22 |
| Issue: |
8 |
| Start Page: |
2173 |
| End Page: |
2185 |