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Antagonistic activity of Phaeobacter piscinae against the emerging fish pathogen Vibrio crassostreae in aquaculture feed algae
,
Despoina Athena-Vasileiadi,
Lone Gram ,
Eva C. Sonnenschein
Applied and Environmental Microbiology, Volume: 90, Issue: 3
Swansea University Author:
Eva C. Sonnenschein
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DOI (Published version): 10.1128/aem.01439-23
Abstract
Aquaculture provides a rich resource of high-quality protein; however, the production is challenged by emerging pathogens such as Vibrio crassostreae. While probiotic bacteria have been proposed as a sustainable solution to reduce pathogen load in aquaculture, their application requires a comprehens...
| Published in: | Applied and Environmental Microbiology |
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| ISSN: | 0099-2240 1098-5336 |
| Published: |
American Society for Microbiology
2024
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| Online Access: |
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| URI: | https://cronfa.swan.ac.uk/Record/cronfa66579 |
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| Abstract: |
Aquaculture provides a rich resource of high-quality protein; however, the production is challenged by emerging pathogens such as Vibrio crassostreae. While probiotic bacteria have been proposed as a sustainable solution to reduce pathogen load in aquaculture, their application requires a comprehensive assessment across the aquaculture food chain. The purpose of this study was to determine the antagonistic effect of the potential probiotic bacterium Phaeobacter piscinae against the emerging fish pathogen V. crassostreae in aquaculture feed algae that can be an entry point for pathogens in fish and shellfish aquaculture. P. piscinae strain S26 produces the antibacterial compound tropodithietic acid (TDA). In a plate-based assay, P. piscinae S26 was equally to more effective than the well-studied Phaeobacter inhibens DSM17395 in its inhibition of the fish pathogens Vibrio anguillarum 90-11-286 and V. crassostreae DMC-1. When co-cultured with the microalgae Tetraselmis suecica and Isochrysis galbana, P. piscinae S26 reduced the maximum cell density of V. crassostreae DMC-1 by 2 log and 3–4 log fold, respectively. A TDA-deficient mutant of P. piscinae S26 inhibited V. crassostreae DMC-1 to a lesser extent than the wild type, suggesting that the antagonistic effect involves TDA and other factors. TDA is the prime antagonistic agent of the inhibition of V. anguillarum 90-11-286. Comparative genomics of V. anguillarum 90-11-286 and V. crassostreae DMC-1 revealed that V. crassostreae DMC-1 carries a greater arsenal of antibiotic resistance genes potentially contributing to the reduced effect of TDA. In conclusion, P. piscinae S26 is a promising new candidate for inhibition of emerging pathogens such as V. crassostreae DMC-1 in algal feed systems and could contribute to a more sustainable aquaculture industry. |
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| Keywords: |
aquaculture, probiotics, fish pathogens, microalgae |
| College: |
Faculty of Science and Engineering |
| Funders: |
This work was supported by the Danish National Research Foundation to the Center for Microbial Secondary Metabolites (DNRF137) and Novo Nordisk Foundation (grant numbers NNF20OC0064249 [EngAlMi] and NNF19OC0055625 [IMLiB]). |
| Issue: |
3 |