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Impact of host species on assembly, composition, and functional profiles of phycosphere microbiomes

Line Roager Orcid Logo, Paul J. Kempen, Mikkel Bentzon-Tilia Orcid Logo, Eva C. Sonnenschein Orcid Logo, Lone Gram Orcid Logo

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Swansea University Author: Eva C. Sonnenschein Orcid Logo

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DOI (Published version): 10.1101/2023.11.08.566273

Abstract

Microalgal microbiomes play vital roles in the growth and health of their host, however, their composition and functions remain only partially characterized, especially across microalgal phyla. In this study, a natural seawater microbiome was introduced to three distinct, axenic species of microalga...

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Published: Cold Spring Harbor Laboratory
URI: https://cronfa.swan.ac.uk/Record/cronfa66581
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spelling v2 66581 2024-06-03 Impact of host species on assembly, composition, and functional profiles of phycosphere microbiomes f6a4027578a15ea3e6453a54b849c686 0000-0001-6959-5100 Eva C. Sonnenschein Eva C. Sonnenschein true false 2024-06-03 BGPS Microalgal microbiomes play vital roles in the growth and health of their host, however, their composition and functions remain only partially characterized, especially across microalgal phyla. In this study, a natural seawater microbiome was introduced to three distinct, axenic species of microalgae, the haptophyte Isochrysis galbana, the chlorophyte Tetraselmis suecica, and the diatom Conticribra weissflogii (previously Thalassiosira), and its divergence and assembly was monitored over 49 days using 16S rRNA amplicon and metagenomic analyses. The microbiomes had a high degree of host specificity in terms of taxonomic composition and potential functions, including CAZymes profiles. Rhodobacteraceae and Flavobacteriaceae families were abundant across all microalgal hosts, but I .galbana microbiomes diverged further from T. suecica and C. weissflogii microbiomes. I .galbana microbiomes had a much higher relative abundance of Flavobacteriaceae, whereas the two other algal microbiomes had higher relative abundances of Rhodobacteraceae. This could be due to the mixotrophic nature of I. galbana affecting the carbohydrate composition available to the microbiomes, which was supported by the CAZymes profile of I. galbana microbiomes diverging further from those of T. suecica and C. weissflogii microbiomes. Finally, the presence of denitrification and other anaerobic pathways was found exclusively in microbiomes of C. weissflogii potentially resulting from anoxic microenvironments in aggregates formed by this diatom during the experiment. These results underline the deterministic role of the microalgal host species on microbiome composition and functional profiles along with other factors, such as trophic mode of the microalgal host. Journal Article bioRxiv Cold Spring Harbor Laboratory 0 0 0 0001-01-01 10.1101/2023.11.08.566273 Preprint article before certification by peer review. COLLEGE NANME Biosciences Geography and Physics School COLLEGE CODE BGPS Swansea University 2024-07-24T13:56:38.0575205 2024-06-03T14:15:03.1902421 Faculty of Science and Engineering School of Biosciences, Geography and Physics - Biosciences Line Roager 0000-0002-7033-7309 1 Paul J. Kempen 2 Mikkel Bentzon-Tilia 0000-0002-7888-9845 3 Eva C. Sonnenschein 0000-0001-6959-5100 4 Lone Gram 0000-0002-1076-5723 5
title Impact of host species on assembly, composition, and functional profiles of phycosphere microbiomes
spellingShingle Impact of host species on assembly, composition, and functional profiles of phycosphere microbiomes
Eva C. Sonnenschein
title_short Impact of host species on assembly, composition, and functional profiles of phycosphere microbiomes
title_full Impact of host species on assembly, composition, and functional profiles of phycosphere microbiomes
title_fullStr Impact of host species on assembly, composition, and functional profiles of phycosphere microbiomes
title_full_unstemmed Impact of host species on assembly, composition, and functional profiles of phycosphere microbiomes
title_sort Impact of host species on assembly, composition, and functional profiles of phycosphere microbiomes
author_id_str_mv f6a4027578a15ea3e6453a54b849c686
author_id_fullname_str_mv f6a4027578a15ea3e6453a54b849c686_***_Eva C. Sonnenschein
author Eva C. Sonnenschein
author2 Line Roager
Paul J. Kempen
Mikkel Bentzon-Tilia
Eva C. Sonnenschein
Lone Gram
format Journal article
container_title bioRxiv
institution Swansea University
doi_str_mv 10.1101/2023.11.08.566273
publisher Cold Spring Harbor Laboratory
college_str Faculty of Science and Engineering
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hierarchy_top_id facultyofscienceandengineering
hierarchy_top_title Faculty of Science and Engineering
hierarchy_parent_id facultyofscienceandengineering
hierarchy_parent_title Faculty of Science and Engineering
department_str School of Biosciences, Geography and Physics - Biosciences{{{_:::_}}}Faculty of Science and Engineering{{{_:::_}}}School of Biosciences, Geography and Physics - Biosciences
document_store_str 0
active_str 0
description Microalgal microbiomes play vital roles in the growth and health of their host, however, their composition and functions remain only partially characterized, especially across microalgal phyla. In this study, a natural seawater microbiome was introduced to three distinct, axenic species of microalgae, the haptophyte Isochrysis galbana, the chlorophyte Tetraselmis suecica, and the diatom Conticribra weissflogii (previously Thalassiosira), and its divergence and assembly was monitored over 49 days using 16S rRNA amplicon and metagenomic analyses. The microbiomes had a high degree of host specificity in terms of taxonomic composition and potential functions, including CAZymes profiles. Rhodobacteraceae and Flavobacteriaceae families were abundant across all microalgal hosts, but I .galbana microbiomes diverged further from T. suecica and C. weissflogii microbiomes. I .galbana microbiomes had a much higher relative abundance of Flavobacteriaceae, whereas the two other algal microbiomes had higher relative abundances of Rhodobacteraceae. This could be due to the mixotrophic nature of I. galbana affecting the carbohydrate composition available to the microbiomes, which was supported by the CAZymes profile of I. galbana microbiomes diverging further from those of T. suecica and C. weissflogii microbiomes. Finally, the presence of denitrification and other anaerobic pathways was found exclusively in microbiomes of C. weissflogii potentially resulting from anoxic microenvironments in aggregates formed by this diatom during the experiment. These results underline the deterministic role of the microalgal host species on microbiome composition and functional profiles along with other factors, such as trophic mode of the microalgal host.
published_date 0001-01-01T13:56:37Z
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