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Long-term tracking of the microbiology of uranium-amended water-saturated bentonite microcosms: a mechanistic characterization of U speciation

Mar Morales-Hidalgo, Cristina Povedano-Priego, Marcos F. Martinez-Moreno, Jesus Ojeda Ledo Orcid Logo, Fadwa Jroundi, Mohamed L. Merroun

Journal of Hazardous Materials, Volume: 476, Start page: 135044

Swansea University Author: Jesus Ojeda Ledo Orcid Logo

DOI (Published version): 10.1016/j.jhazmat.2024.135044

Abstract

Deep geological repositories (DGRs) stand out as one of the optimal options for managing high-level radioactive waste (HLW) such as uranium (U) in the near future. Here, we provide novel insights into microbial behavior in the DGR bentonite barrier, addressing potential worst-case scenarios such as...

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Published in: Journal of Hazardous Materials
Published: Elsevier 2024
URI: https://cronfa.swan.ac.uk/Record/cronfa66892
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spelling v2 66892 2024-06-25 Long-term tracking of the microbiology of uranium-amended water-saturated bentonite microcosms: a mechanistic characterization of U speciation 4c1c9800dffa623353dff0ab1271be64 0000-0002-2046-1010 Jesus Ojeda Ledo Jesus Ojeda Ledo true false 2024-06-25 EAAS Deep geological repositories (DGRs) stand out as one of the optimal options for managing high-level radioactive waste (HLW) such as uranium (U) in the near future. Here, we provide novel insights into microbial behavior in the DGR bentonite barrier, addressing potential worst-case scenarios such as waste leakage (e.g., U) and groundwater infiltration of electron rich donors in the bentonite. After a three-year anaerobic incubation, Illumina sequencing results revealed a bacterial diversity dominated by anaerobic and spore-forming micro-organisms mainly from the phylum Firmicutes. Highly U tolerant and viable bacterial isolates from the genera Peribacillus, Bacillus, and some SRB such as Desulfovibrio and Desulfosporosinus, were enriched from U-amended bentonite. The results obtained by XPS and XRD showed that U was present as U(VI) and as U(IV) species. Regarding U(VI), we have identified biogenic U(VI) phosphates, U(UO2)·(PO4)2, located in the inner part of the bacterial cell membranes in addition to U(VI)-adsorbed to clays such as montmorillonite. Biogenic U(IV) species as uraninite may be produced as result of bacterial enzymatic U(VI) reduction. These findings suggest that under electron donor-rich water-saturation conditions, bentonite microbial community can control U speciation, immobilizing it, and thus enhancing future DGR safety if container rupture and waste leakage occurs. Journal Article Journal of Hazardous Materials 476 135044 Elsevier DGR; bentonite slurry; uranium; long-term incubation, microbial diversity; sulfate reducing bacteria 5 9 2024 2024-09-05 10.1016/j.jhazmat.2024.135044 COLLEGE NANME Engineering and Applied Sciences School COLLEGE CODE EAAS Swansea University Another institution paid the OA fee This study was supported by the grant RTI2018–101548-B-I00 “ERDF A way of making Europe” to MLM from the “Ministerio de Ciencia, Innovación y Universidades” (Spanish Government). The project leading to this application has received funding from the European Union's Horizon 2020 research and innovation program under grant agreement No 847593 to MLM and the grant FPU20/00583 to the first author from the “Ministerio de Universidades” (Spanish Government). 2024-06-29T17:23:49.2887306 2024-06-25T16:16:15.4309313 Faculty of Science and Engineering School of Engineering and Applied Sciences - Chemical Engineering Mar Morales-Hidalgo 1 Cristina Povedano-Priego 2 Marcos F. Martinez-Moreno 3 Jesus Ojeda Ledo 0000-0002-2046-1010 4 Fadwa Jroundi 5 Mohamed L. Merroun 6 66892__30781__02e6155077a54e0aa0c0b110d243851a.pdf 1-s2.0-S0304389424016236-main.pdf 2024-06-29T17:19:54.3240930 Output 8839777 application/pdf Version of Record true false
title Long-term tracking of the microbiology of uranium-amended water-saturated bentonite microcosms: a mechanistic characterization of U speciation
spellingShingle Long-term tracking of the microbiology of uranium-amended water-saturated bentonite microcosms: a mechanistic characterization of U speciation
Jesus Ojeda Ledo
title_short Long-term tracking of the microbiology of uranium-amended water-saturated bentonite microcosms: a mechanistic characterization of U speciation
title_full Long-term tracking of the microbiology of uranium-amended water-saturated bentonite microcosms: a mechanistic characterization of U speciation
title_fullStr Long-term tracking of the microbiology of uranium-amended water-saturated bentonite microcosms: a mechanistic characterization of U speciation
title_full_unstemmed Long-term tracking of the microbiology of uranium-amended water-saturated bentonite microcosms: a mechanistic characterization of U speciation
title_sort Long-term tracking of the microbiology of uranium-amended water-saturated bentonite microcosms: a mechanistic characterization of U speciation
author_id_str_mv 4c1c9800dffa623353dff0ab1271be64
author_id_fullname_str_mv 4c1c9800dffa623353dff0ab1271be64_***_Jesus Ojeda Ledo
author Jesus Ojeda Ledo
author2 Mar Morales-Hidalgo
Cristina Povedano-Priego
Marcos F. Martinez-Moreno
Jesus Ojeda Ledo
Fadwa Jroundi
Mohamed L. Merroun
format Journal article
container_title Journal of Hazardous Materials
container_volume 476
container_start_page 135044
publishDate 2024
institution Swansea University
doi_str_mv 10.1016/j.jhazmat.2024.135044
publisher Elsevier
college_str Faculty of Science and Engineering
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hierarchy_top_id facultyofscienceandengineering
hierarchy_top_title Faculty of Science and Engineering
hierarchy_parent_id facultyofscienceandengineering
hierarchy_parent_title Faculty of Science and Engineering
department_str School of Engineering and Applied Sciences - Chemical Engineering{{{_:::_}}}Faculty of Science and Engineering{{{_:::_}}}School of Engineering and Applied Sciences - Chemical Engineering
document_store_str 1
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description Deep geological repositories (DGRs) stand out as one of the optimal options for managing high-level radioactive waste (HLW) such as uranium (U) in the near future. Here, we provide novel insights into microbial behavior in the DGR bentonite barrier, addressing potential worst-case scenarios such as waste leakage (e.g., U) and groundwater infiltration of electron rich donors in the bentonite. After a three-year anaerobic incubation, Illumina sequencing results revealed a bacterial diversity dominated by anaerobic and spore-forming micro-organisms mainly from the phylum Firmicutes. Highly U tolerant and viable bacterial isolates from the genera Peribacillus, Bacillus, and some SRB such as Desulfovibrio and Desulfosporosinus, were enriched from U-amended bentonite. The results obtained by XPS and XRD showed that U was present as U(VI) and as U(IV) species. Regarding U(VI), we have identified biogenic U(VI) phosphates, U(UO2)·(PO4)2, located in the inner part of the bacterial cell membranes in addition to U(VI)-adsorbed to clays such as montmorillonite. Biogenic U(IV) species as uraninite may be produced as result of bacterial enzymatic U(VI) reduction. These findings suggest that under electron donor-rich water-saturation conditions, bentonite microbial community can control U speciation, immobilizing it, and thus enhancing future DGR safety if container rupture and waste leakage occurs.
published_date 2024-09-05T17:23:47Z
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score 11.012678

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