No Cover Image

Journal article 323 views 32 downloads

Mechano-bactericidal activity of two-photon polymerized micro-and nanoscale topographies against Pseudomonas aeruginosa: Surface interactions and antibacterial efficacy

Ning Tan, Jisun Im, Nigel Neate, Chee-Onn Leong, Ricky D. Wildman, Georgina Marsh, Maxine Swee-Li Yee

Materials Today Communications, Volume: 40, Start page: 109785

Swansea University Author: Georgina Marsh

  • 67008.VoR.pdf

    PDF | Version of Record

    © 2024 The Author(s). This is an open access article under the CC BY license.

    Download (8.92MB)

Check full text

DOI (Published version): 10.1016/j.mtcomm.2024.109785

Abstract

The emergence of antimicrobial-resistant bacteria poses a significant health concern, stemming from chemically induced intrinsic and acquired-resistance responses in the microbe. Nanopatterns are an alternative bactericidal approach, employing physical features to prevent biofilm formation and kill...

Full description

Published in: Materials Today Communications
ISSN: 2352-4928
Published: Elsevier BV 2024
Online Access: Check full text

URI: https://cronfa.swan.ac.uk/Record/cronfa67008
first_indexed 2024-07-09T10:17:21Z
last_indexed 2024-11-25T14:19:21Z
id cronfa67008
recordtype SURis
fullrecord <?xml version="1.0"?><rfc1807><datestamp>2024-09-02T15:30:06.9638243</datestamp><bib-version>v2</bib-version><id>67008</id><entry>2024-07-09</entry><title>Mechano-bactericidal activity of two-photon polymerized micro-and nanoscale topographies against Pseudomonas aeruginosa: Surface interactions and antibacterial efficacy</title><swanseaauthors><author><sid>60eb67dc80ac6072cbea39caa88d662c</sid><firstname>Georgina</firstname><surname>Marsh</surname><name>Georgina Marsh</name><active>true</active><ethesisStudent>false</ethesisStudent></author></swanseaauthors><date>2024-07-09</date><deptcode>ONDF</deptcode><abstract>The emergence of antimicrobial-resistant bacteria poses a significant health concern, stemming from chemically induced intrinsic and acquired-resistance responses in the microbe. Nanopatterns are an alternative bactericidal approach, employing physical features to prevent biofilm formation and kill bacteria. This work draws inspiration from the natural mechano-bactericidal properties of sub-micron scale surface structures present on cicada wings, with the fabrication of synthetic, chemically-inert surfaces using the two-photon polymerization (2PP) technique. In contrast to the random packing and distribution of the nanotopography of cicada wings, the 2PP synthetic surfaces were produced with highly uniform and precise surface geometries of nanopillars and micropillars. These synthetic topographies with hexagonal-arranged nano/micro features induced a spacing-dependent response in Pseudomonas aeruginosa, influencing their cell viability, adhesion property and biofilm formation ability. Optimized spacings &#x223C;500&#x202F;nm between nanopillars were associated with higher proportions of distorted and ruptured bacteria cells, while up to 60&#x202F;% reduction of biofilms were observed on micropillared surfaces with &#x223C;2 micron spacings between pillars. Whole transcriptome analysis of bacteria exposed to the synthetic surface indicated significant upregulation of a single pathway associated with quorum sensing. The PA3305.1 pathway induced quinolone signal synthesis in P. aeruginosa. The findings of this study establish the basis for developing complex antimicrobial surfaces using a physical approach, without reliance on chemical means.</abstract><type>Journal Article</type><journal>Materials Today Communications</journal><volume>40</volume><journalNumber/><paginationStart>109785</paginationStart><paginationEnd/><publisher>Elsevier BV</publisher><placeOfPublication/><isbnPrint/><isbnElectronic/><issnPrint>2352-4928</issnPrint><issnElectronic/><keywords>Bactericidal; Synthetic micro-/nano-topography; Two-photon polymerization; Antibacterial surface</keywords><publishedDay>1</publishedDay><publishedMonth>8</publishedMonth><publishedYear>2024</publishedYear><publishedDate>2024-08-01</publishedDate><doi>10.1016/j.mtcomm.2024.109785</doi><url/><notes/><college>COLLEGE NANME</college><department>Other/Subsidiary Companies - Not Defined</department><CollegeCode>COLLEGE CODE</CollegeCode><DepartmentCode>ONDF</DepartmentCode><institution>Swansea University</institution><apcterm>Another institution paid the OA fee</apcterm><funders>This research was funded by Ministry of Higher Education Malaysia FRGS/1/2020/TK0/UNIM/03/3 and UNM FOSE internal grant LA744019.</funders><projectreference/><lastEdited>2024-09-02T15:30:06.9638243</lastEdited><Created>2024-07-09T11:14:37.1166854</Created><path><level id="1">Faculty of Medicine, Health and Life Sciences</level><level id="2">Swansea University Medical School - Pharmacy</level></path><authors><author><firstname>Ning</firstname><surname>Tan</surname><order>1</order></author><author><firstname>Jisun</firstname><surname>Im</surname><order>2</order></author><author><firstname>Nigel</firstname><surname>Neate</surname><order>3</order></author><author><firstname>Chee-Onn</firstname><surname>Leong</surname><order>4</order></author><author><firstname>Ricky D.</firstname><surname>Wildman</surname><order>5</order></author><author><firstname>Georgina</firstname><surname>Marsh</surname><order>6</order></author><author><firstname>Maxine Swee-Li</firstname><surname>Yee</surname><order>7</order></author></authors><documents><document><filename>67008__31230__7a32d793917546aa831a7cc0a6c415b1.pdf</filename><originalFilename>67008.VoR.pdf</originalFilename><uploaded>2024-09-02T15:26:06.9806050</uploaded><type>Output</type><contentLength>9348633</contentLength><contentType>application/pdf</contentType><version>Version of Record</version><cronfaStatus>true</cronfaStatus><documentNotes>&#xA9; 2024 The Author(s). This is an open access article under the CC BY license.</documentNotes><copyrightCorrect>true</copyrightCorrect><language>eng</language><licence>http://creativecommons.org/licenses/by/4.0/</licence></document></documents><OutputDurs/></rfc1807>
spelling 2024-09-02T15:30:06.9638243 v2 67008 2024-07-09 Mechano-bactericidal activity of two-photon polymerized micro-and nanoscale topographies against Pseudomonas aeruginosa: Surface interactions and antibacterial efficacy 60eb67dc80ac6072cbea39caa88d662c Georgina Marsh Georgina Marsh true false 2024-07-09 ONDF The emergence of antimicrobial-resistant bacteria poses a significant health concern, stemming from chemically induced intrinsic and acquired-resistance responses in the microbe. Nanopatterns are an alternative bactericidal approach, employing physical features to prevent biofilm formation and kill bacteria. This work draws inspiration from the natural mechano-bactericidal properties of sub-micron scale surface structures present on cicada wings, with the fabrication of synthetic, chemically-inert surfaces using the two-photon polymerization (2PP) technique. In contrast to the random packing and distribution of the nanotopography of cicada wings, the 2PP synthetic surfaces were produced with highly uniform and precise surface geometries of nanopillars and micropillars. These synthetic topographies with hexagonal-arranged nano/micro features induced a spacing-dependent response in Pseudomonas aeruginosa, influencing their cell viability, adhesion property and biofilm formation ability. Optimized spacings ∼500 nm between nanopillars were associated with higher proportions of distorted and ruptured bacteria cells, while up to 60 % reduction of biofilms were observed on micropillared surfaces with ∼2 micron spacings between pillars. Whole transcriptome analysis of bacteria exposed to the synthetic surface indicated significant upregulation of a single pathway associated with quorum sensing. The PA3305.1 pathway induced quinolone signal synthesis in P. aeruginosa. The findings of this study establish the basis for developing complex antimicrobial surfaces using a physical approach, without reliance on chemical means. Journal Article Materials Today Communications 40 109785 Elsevier BV 2352-4928 Bactericidal; Synthetic micro-/nano-topography; Two-photon polymerization; Antibacterial surface 1 8 2024 2024-08-01 10.1016/j.mtcomm.2024.109785 COLLEGE NANME Other/Subsidiary Companies - Not Defined COLLEGE CODE ONDF Swansea University Another institution paid the OA fee This research was funded by Ministry of Higher Education Malaysia FRGS/1/2020/TK0/UNIM/03/3 and UNM FOSE internal grant LA744019. 2024-09-02T15:30:06.9638243 2024-07-09T11:14:37.1166854 Faculty of Medicine, Health and Life Sciences Swansea University Medical School - Pharmacy Ning Tan 1 Jisun Im 2 Nigel Neate 3 Chee-Onn Leong 4 Ricky D. Wildman 5 Georgina Marsh 6 Maxine Swee-Li Yee 7 67008__31230__7a32d793917546aa831a7cc0a6c415b1.pdf 67008.VoR.pdf 2024-09-02T15:26:06.9806050 Output 9348633 application/pdf Version of Record true © 2024 The Author(s). This is an open access article under the CC BY license. true eng http://creativecommons.org/licenses/by/4.0/
title Mechano-bactericidal activity of two-photon polymerized micro-and nanoscale topographies against Pseudomonas aeruginosa: Surface interactions and antibacterial efficacy
spellingShingle Mechano-bactericidal activity of two-photon polymerized micro-and nanoscale topographies against Pseudomonas aeruginosa: Surface interactions and antibacterial efficacy
Georgina Marsh
title_short Mechano-bactericidal activity of two-photon polymerized micro-and nanoscale topographies against Pseudomonas aeruginosa: Surface interactions and antibacterial efficacy
title_full Mechano-bactericidal activity of two-photon polymerized micro-and nanoscale topographies against Pseudomonas aeruginosa: Surface interactions and antibacterial efficacy
title_fullStr Mechano-bactericidal activity of two-photon polymerized micro-and nanoscale topographies against Pseudomonas aeruginosa: Surface interactions and antibacterial efficacy
title_full_unstemmed Mechano-bactericidal activity of two-photon polymerized micro-and nanoscale topographies against Pseudomonas aeruginosa: Surface interactions and antibacterial efficacy
title_sort Mechano-bactericidal activity of two-photon polymerized micro-and nanoscale topographies against Pseudomonas aeruginosa: Surface interactions and antibacterial efficacy
author_id_str_mv 60eb67dc80ac6072cbea39caa88d662c
author_id_fullname_str_mv 60eb67dc80ac6072cbea39caa88d662c_***_Georgina Marsh
author Georgina Marsh
author2 Ning Tan
Jisun Im
Nigel Neate
Chee-Onn Leong
Ricky D. Wildman
Georgina Marsh
Maxine Swee-Li Yee
format Journal article
container_title Materials Today Communications
container_volume 40
container_start_page 109785
publishDate 2024
institution Swansea University
issn 2352-4928
doi_str_mv 10.1016/j.mtcomm.2024.109785
publisher Elsevier BV
college_str Faculty of Medicine, Health and Life Sciences
hierarchytype
hierarchy_top_id facultyofmedicinehealthandlifesciences
hierarchy_top_title Faculty of Medicine, Health and Life Sciences
hierarchy_parent_id facultyofmedicinehealthandlifesciences
hierarchy_parent_title Faculty of Medicine, Health and Life Sciences
department_str Swansea University Medical School - Pharmacy{{{_:::_}}}Faculty of Medicine, Health and Life Sciences{{{_:::_}}}Swansea University Medical School - Pharmacy
document_store_str 1
active_str 0
description The emergence of antimicrobial-resistant bacteria poses a significant health concern, stemming from chemically induced intrinsic and acquired-resistance responses in the microbe. Nanopatterns are an alternative bactericidal approach, employing physical features to prevent biofilm formation and kill bacteria. This work draws inspiration from the natural mechano-bactericidal properties of sub-micron scale surface structures present on cicada wings, with the fabrication of synthetic, chemically-inert surfaces using the two-photon polymerization (2PP) technique. In contrast to the random packing and distribution of the nanotopography of cicada wings, the 2PP synthetic surfaces were produced with highly uniform and precise surface geometries of nanopillars and micropillars. These synthetic topographies with hexagonal-arranged nano/micro features induced a spacing-dependent response in Pseudomonas aeruginosa, influencing their cell viability, adhesion property and biofilm formation ability. Optimized spacings ∼500 nm between nanopillars were associated with higher proportions of distorted and ruptured bacteria cells, while up to 60 % reduction of biofilms were observed on micropillared surfaces with ∼2 micron spacings between pillars. Whole transcriptome analysis of bacteria exposed to the synthetic surface indicated significant upregulation of a single pathway associated with quorum sensing. The PA3305.1 pathway induced quinolone signal synthesis in P. aeruginosa. The findings of this study establish the basis for developing complex antimicrobial surfaces using a physical approach, without reliance on chemical means.
published_date 2024-08-01T08:31:44Z
_version_ 1830811918059700224
score 11.061153

Similar Items