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Minimal information for studies of extracellular vesicles (MISEV2023): From basic to advanced approaches
Joshua A. Welsh 
,
Deborah C. I. Goberdhan ,
Lorraine O'Driscoll,
Edit I. Buzas ,
Cherie Blenkiron,
Benedetta Bussolati ,
Houjian Cai ,
Dolores Di Vizio,
Tom A. P. Driedonks,
Uta Erdbrügger,
Juan M. Falcon‐Perez ,
Qing‐Ling Fu,
Andrew F. Hill ,
Metka Lenassi ,
Sai Kiang Lim,
Mỹ G. Mahoney,
Sujata Mohanty ,
Andreas Möller,
Rienk Nieuwland ,
Takahiro Ochiya ,
Susmita Sahoo ,
Ana C. Torrecilhas ,
Lei Zheng ,
Andries Zijlstra ,
Sarah Abuelreich,
Reem Bagabas,
Paolo Bergese,
Esther M. Bridges,
Marco Brucale ,
Dylan Burger,
Randy P. Carney ,
Emanuele Cocucci,
Rossella Crescitelli ,
Edveena Hanser,
Adrian L. Harris,
Norman J. Haughey ,
An Hendrix,
Alexander R. Ivanov,
Tijana Jovanovic‐Talisman,
Nicole A. Kruh‐Garcia,
Vroniqa Ku'ulei‐Lyn Faustino,
Diego Kyburz,
Cecilia Lässer,
Kathleen M. Lennon,
Jan Lötvall,
Adam L. Maddox,
Elena S. Martens‐Uzunova ,
Rachel R. Mizenko,
Lauren A. Newman ,
Andrea Ridolfi,
Eva Rohde ,
Tatu Rojalin,
Andrew Rowland,
Andras Saftics,
Ursula S. Sandau ,
Julie A. Saugstad ,
Faezeh Shekari ,
Simon Swift,
Dmitry Ter‐Ovanesyan,
Juan P. Tosar ,
Zivile Useckaite,
Francesco Valle,
Zoltan Varga,
Edwin van der Pol,
Martijn J. C. van Herwijnen,
Marca H. M. Wauben ,
Ann M. Wehman ,
Sarah Williams,
Andrea Zendrini,
Alan J. Zimmerman ,
(MISEV Consortium),
Clotilde Théry ,
Kenneth W. Witwer ,
Jason Webber
,
Deborah C. I. Goberdhan ,
Lorraine O'Driscoll,
Edit I. Buzas ,
Cherie Blenkiron,
Benedetta Bussolati ,
Houjian Cai ,
Dolores Di Vizio,
Tom A. P. Driedonks,
Uta Erdbrügger,
Juan M. Falcon‐Perez ,
Qing‐Ling Fu,
Andrew F. Hill ,
Metka Lenassi ,
Sai Kiang Lim,
Mỹ G. Mahoney,
Sujata Mohanty ,
Andreas Möller,
Rienk Nieuwland ,
Takahiro Ochiya ,
Susmita Sahoo ,
Ana C. Torrecilhas ,
Lei Zheng ,
Andries Zijlstra ,
Sarah Abuelreich,
Reem Bagabas,
Paolo Bergese,
Esther M. Bridges,
Marco Brucale ,
Dylan Burger,
Randy P. Carney ,
Emanuele Cocucci,
Rossella Crescitelli ,
Edveena Hanser,
Adrian L. Harris,
Norman J. Haughey ,
An Hendrix,
Alexander R. Ivanov,
Tijana Jovanovic‐Talisman,
Nicole A. Kruh‐Garcia,
Vroniqa Ku'ulei‐Lyn Faustino,
Diego Kyburz,
Cecilia Lässer,
Kathleen M. Lennon,
Jan Lötvall,
Adam L. Maddox,
Elena S. Martens‐Uzunova ,
Rachel R. Mizenko,
Lauren A. Newman ,
Andrea Ridolfi,
Eva Rohde ,
Tatu Rojalin,
Andrew Rowland,
Andras Saftics,
Ursula S. Sandau ,
Julie A. Saugstad ,
Faezeh Shekari ,
Simon Swift,
Dmitry Ter‐Ovanesyan,
Juan P. Tosar ,
Zivile Useckaite,
Francesco Valle,
Zoltan Varga,
Edwin van der Pol,
Martijn J. C. van Herwijnen,
Marca H. M. Wauben ,
Ann M. Wehman ,
Sarah Williams,
Andrea Zendrini,
Alan J. Zimmerman ,
(MISEV Consortium),
Clotilde Théry ,
Kenneth W. Witwer ,
Jason Webber
Journal of Extracellular Vesicles, Volume: 13, Issue: 2
Swansea University Author:
Jason Webber
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DOI (Published version): 10.1002/jev2.12404
Abstract
Extracellular vesicles (EVs), through their complex cargo, can reflect the state of their cell of origin and change the functions and phenotypes of other cells. These features indicate strong biomarker and therapeutic potential and have generated broad interest, as evidenced by the steady year-on-ye...
| Published in: | Journal of Extracellular Vesicles |
|---|---|
| ISSN: | 2001-3078 2001-3078 |
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Wiley
2024
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| URI: | https://cronfa.swan.ac.uk/Record/cronfa67053 |
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These features indicate strong biomarker and therapeutic potential and have generated broad interest, as evidenced by the steady year-on-year increase in the numbers of scientific publications about EVs. Important advances have been made in EV metrology and in understanding and applying EV biology. However, hurdles remain to realising the potential of EVs in domains ranging from basic biology to clinical applications due to challenges in EV nomenclature, separation from non-vesicular extracellular particles, characterisation and functional studies. To address the challenges and opportunities in this rapidly evolving field, the International Society for Extracellular Vesicles (ISEV) updates its ‘Minimal Information for Studies of Extracellular Vesicles’, which was first published in 2014 and then in 2018 as MISEV2014 and MISEV2018, respectively. The goal of the current document, MISEV2023, is to provide researchers with an updated snapshot of available approaches and their advantages and limitations for production, separation and characterisation of EVs from multiple sources, including cell culture, body fluids and solid tissues. In addition to presenting the latest state of the art in basic principles of EV research, this document also covers advanced techniques and approaches that are currently expanding the boundaries of the field. MISEV2023 also includes new sections on EV release and uptake and a brief discussion of in vivo approaches to study EVs. 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v2 67053 2024-07-09 Minimal information for studies of extracellular vesicles (MISEV2023): From basic to advanced approaches 25d1a26f9b8bb556bd9412080e40351d 0000-0003-4772-3014 Jason Webber Jason Webber true false 2024-07-09 MEDS Extracellular vesicles (EVs), through their complex cargo, can reflect the state of their cell of origin and change the functions and phenotypes of other cells. These features indicate strong biomarker and therapeutic potential and have generated broad interest, as evidenced by the steady year-on-year increase in the numbers of scientific publications about EVs. Important advances have been made in EV metrology and in understanding and applying EV biology. However, hurdles remain to realising the potential of EVs in domains ranging from basic biology to clinical applications due to challenges in EV nomenclature, separation from non-vesicular extracellular particles, characterisation and functional studies. To address the challenges and opportunities in this rapidly evolving field, the International Society for Extracellular Vesicles (ISEV) updates its ‘Minimal Information for Studies of Extracellular Vesicles’, which was first published in 2014 and then in 2018 as MISEV2014 and MISEV2018, respectively. The goal of the current document, MISEV2023, is to provide researchers with an updated snapshot of available approaches and their advantages and limitations for production, separation and characterisation of EVs from multiple sources, including cell culture, body fluids and solid tissues. In addition to presenting the latest state of the art in basic principles of EV research, this document also covers advanced techniques and approaches that are currently expanding the boundaries of the field. MISEV2023 also includes new sections on EV release and uptake and a brief discussion of in vivo approaches to study EVs. Compiling feedback from ISEV expert task forces and more than 1000 researchers, this document conveys the current state of EV research to facilitate robust scientific discoveries and move the field forward even more rapidly. Journal Article Journal of Extracellular Vesicles 13 2 Wiley 2001-3078 2001-3078 ectosomes, exosomes, extracellular vesicles, extracellular particles, guidelines, microparticles, microvesicles, minimal information requirements, MISEV, reproducibility, rigor, standardisation 1 2 2024 2024-02-01 10.1002/jev2.12404 Correction to “Minimal information for studies of extracellular vesicles (MISEV2023): From basic to advanced approaches” available at https://doi.org/10.1002/jev2.12451 COLLEGE NANME Medical School COLLEGE CODE MEDS Swansea University 2024-09-02T11:17:45.5144514 2024-07-09T15:35:36.1367682 Faculty of Medicine, Health and Life Sciences Swansea University Medical School - Biomedical Science Joshua A. Welsh 0000-0002-1097-9756 1 Deborah C. I. Goberdhan 0000-0003-0645-6714 2 Lorraine O'Driscoll 3 Edit I. Buzas 0000-0002-3744-206x 4 Cherie Blenkiron 5 Benedetta Bussolati 0000-0002-3663-5134 6 Houjian Cai 0000-0003-4887-2652 7 Dolores Di Vizio 8 Tom A. P. Driedonks 9 Uta Erdbrügger 10 Juan M. Falcon‐Perez 0000-0003-3133-0670 11 Qing‐Ling Fu 12 Andrew F. Hill 0000-0001-5581-2354 13 Metka Lenassi 0000-0002-9488-6855 14 Sai Kiang Lim 15 Mỹ G. Mahoney 16 Sujata Mohanty 0000-0002-0047-4914 17 Andreas Möller 18 Rienk Nieuwland 0000-0002-5671-3400 19 Takahiro Ochiya 0000-0002-0776-9918 20 Susmita Sahoo 0000-0002-7279-1564 21 Ana C. Torrecilhas 0000-0001-5724-2199 22 Lei Zheng 0000-0003-2576-8780 23 Andries Zijlstra 0000-0001-8460-8803 24 Sarah Abuelreich 25 Reem Bagabas 26 Paolo Bergese 27 Esther M. Bridges 28 Marco Brucale 0000-0001-7244-4389 29 Dylan Burger 30 Randy P. Carney 0000-0001-8193-1664 31 Emanuele Cocucci 32 Rossella Crescitelli 0000-0002-1714-3169 33 Edveena Hanser 34 Adrian L. Harris 35 Norman J. Haughey 0000-0001-5194-4122 36 An Hendrix 37 Alexander R. Ivanov 38 Tijana Jovanovic‐Talisman 39 Nicole A. Kruh‐Garcia 40 Vroniqa Ku'ulei‐Lyn Faustino 41 Diego Kyburz 42 Cecilia Lässer 43 Kathleen M. Lennon 44 Jan Lötvall 45 Adam L. Maddox 46 Elena S. Martens‐Uzunova 0000-0002-5363-2525 47 Rachel R. Mizenko 48 Lauren A. Newman 0000-0003-3303-1666 49 Andrea Ridolfi 50 Eva Rohde 0000-0001-8692-886x 51 Tatu Rojalin 52 Andrew Rowland 53 Andras Saftics 54 Ursula S. Sandau 0000-0002-3646-7089 55 Julie A. Saugstad 0000-0002-2996-9611 56 Faezeh Shekari 0000-0001-6026-5412 57 Simon Swift 58 Dmitry Ter‐Ovanesyan 59 Juan P. Tosar 0000-0002-2021-2479 60 Zivile Useckaite 61 Francesco Valle 62 Zoltan Varga 63 Edwin van der Pol 64 Martijn J. C. van Herwijnen 65 Marca H. M. Wauben 0000-0003-0360-0311 66 Ann M. Wehman 0000-0001-9826-4132 67 Sarah Williams 68 Andrea Zendrini 69 Alan J. Zimmerman 0000-0001-6280-4790 70 (MISEV Consortium) 71 Clotilde Théry 0000-0001-8294-6884 72 Kenneth W. Witwer 0000-0003-1664-4233 73 Jason Webber 0000-0003-4772-3014 74 67053__31209__fc6e115374424e788c71a15bdc7b1f49.pdf 67053.VoR.pdf 2024-09-02T11:14:50.6654088 Output 2581391 application/pdf Version of Record true © 2024 The Authors. This is an open access article under the terms of the Creative Commons Attribution License. true eng http://creativecommons.org/licenses/by/4.0/ |
| title |
Minimal information for studies of extracellular vesicles (MISEV2023): From basic to advanced approaches |
| spellingShingle |
Minimal information for studies of extracellular vesicles (MISEV2023): From basic to advanced approaches Jason Webber |
| title_short |
Minimal information for studies of extracellular vesicles (MISEV2023): From basic to advanced approaches |
| title_full |
Minimal information for studies of extracellular vesicles (MISEV2023): From basic to advanced approaches |
| title_fullStr |
Minimal information for studies of extracellular vesicles (MISEV2023): From basic to advanced approaches |
| title_full_unstemmed |
Minimal information for studies of extracellular vesicles (MISEV2023): From basic to advanced approaches |
| title_sort |
Minimal information for studies of extracellular vesicles (MISEV2023): From basic to advanced approaches |
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25d1a26f9b8bb556bd9412080e40351d |
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25d1a26f9b8bb556bd9412080e40351d_***_Jason Webber |
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Jason Webber |
| author2 |
Joshua A. Welsh Deborah C. I. Goberdhan Lorraine O'Driscoll Edit I. Buzas Cherie Blenkiron Benedetta Bussolati Houjian Cai Dolores Di Vizio Tom A. P. Driedonks Uta Erdbrügger Juan M. Falcon‐Perez Qing‐Ling Fu Andrew F. Hill Metka Lenassi Sai Kiang Lim Mỹ G. Mahoney Sujata Mohanty Andreas Möller Rienk Nieuwland Takahiro Ochiya Susmita Sahoo Ana C. Torrecilhas Lei Zheng Andries Zijlstra Sarah Abuelreich Reem Bagabas Paolo Bergese Esther M. Bridges Marco Brucale Dylan Burger Randy P. Carney Emanuele Cocucci Rossella Crescitelli Edveena Hanser Adrian L. Harris Norman J. Haughey An Hendrix Alexander R. Ivanov Tijana Jovanovic‐Talisman Nicole A. Kruh‐Garcia Vroniqa Ku'ulei‐Lyn Faustino Diego Kyburz Cecilia Lässer Kathleen M. Lennon Jan Lötvall Adam L. Maddox Elena S. Martens‐Uzunova Rachel R. Mizenko Lauren A. Newman Andrea Ridolfi Eva Rohde Tatu Rojalin Andrew Rowland Andras Saftics Ursula S. Sandau Julie A. Saugstad Faezeh Shekari Simon Swift Dmitry Ter‐Ovanesyan Juan P. Tosar Zivile Useckaite Francesco Valle Zoltan Varga Edwin van der Pol Martijn J. C. van Herwijnen Marca H. M. Wauben Ann M. Wehman Sarah Williams Andrea Zendrini Alan J. Zimmerman (MISEV Consortium) Clotilde Théry Kenneth W. Witwer Jason Webber |
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Journal of Extracellular Vesicles |
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2024 |
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2001-3078 2001-3078 |
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10.1002/jev2.12404 |
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Wiley |
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0 |
| description |
Extracellular vesicles (EVs), through their complex cargo, can reflect the state of their cell of origin and change the functions and phenotypes of other cells. These features indicate strong biomarker and therapeutic potential and have generated broad interest, as evidenced by the steady year-on-year increase in the numbers of scientific publications about EVs. Important advances have been made in EV metrology and in understanding and applying EV biology. However, hurdles remain to realising the potential of EVs in domains ranging from basic biology to clinical applications due to challenges in EV nomenclature, separation from non-vesicular extracellular particles, characterisation and functional studies. To address the challenges and opportunities in this rapidly evolving field, the International Society for Extracellular Vesicles (ISEV) updates its ‘Minimal Information for Studies of Extracellular Vesicles’, which was first published in 2014 and then in 2018 as MISEV2014 and MISEV2018, respectively. The goal of the current document, MISEV2023, is to provide researchers with an updated snapshot of available approaches and their advantages and limitations for production, separation and characterisation of EVs from multiple sources, including cell culture, body fluids and solid tissues. In addition to presenting the latest state of the art in basic principles of EV research, this document also covers advanced techniques and approaches that are currently expanding the boundaries of the field. MISEV2023 also includes new sections on EV release and uptake and a brief discussion of in vivo approaches to study EVs. Compiling feedback from ISEV expert task forces and more than 1000 researchers, this document conveys the current state of EV research to facilitate robust scientific discoveries and move the field forward even more rapidly. |
| published_date |
2024-02-01T11:17:44Z |
| _version_ |
1809079090543591424 |
| score |
11.028798 |