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Effect of early rearing conditions on the behaviour and microbiome of fish with low genetic diversity / ISHRAT ANKA

Swansea University Author: ISHRAT ANKA

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DOI (Published version): 10.23889/SUThesis.67285

Abstract

Fish performance is influenced by their genotype and environment. For populations with low genetic diversity, adaptation to environmental change can be compromised, but it has been suggested that the microbiome can act as an additional source of variability. Early rearing conditions can be particula...

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Published: Swansea University, Wales, UK 2024
Institution: Swansea University
Degree level: Doctoral
Degree name: Ph.D
Supervisor: Consuegra, S., Garcia de Leaniz, C., and Uren Webster, T. M.
URI: https://cronfa.swan.ac.uk/Record/cronfa67285
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Early rearing conditions can be particularly important for fish development and behaviour, due to their dependence on the environmental conditions. This thesis explored the interactions between fish genotype and rearing environment (diet and enrichment) on behaviour, metabolic rate and microbiome (gut, skin) using captive and wild populations of naturally inbred mangrove killifishes (Kryptolebias sp.), where the exploratory behaviour and closed respirometry were used to test the captive killifish and microbiome diversity analyses were performed in both captive and wild killifishes.Behavioural trials in self-fertilizing killifish (Kryptolebias marmoratus) indicated a significant effect of both genetic strain and rearing environment (environmental enrichment and diet) on fish activity measurements. Incubation time also had a substantial role on both fish behaviour and microbiome diversity. Gut-microbiome alpha diversity was shaped by strain, diet, and hatching time in K. marmoratus with interactions between diet and physical enrichment.An intergenerational influence of rearing environment on fish behaviour was detected in K.armoratus, where parental activity was found to influence offspring activity. Gut-microbial comparisons between parents and offspring identified Vibrionaceae as the dominant colonizers in laboratory reared K. marmoratus. A dominant effect of the rearing environment over strain on both microbiome composition and distribution was observed. The influence of the interaction between parental and own environments on microbiome alpha diversity in K. marmoratus offspring suggests long-term effects of the rearing environment on the fish (gut) microbiome.In the wild, results from the outcrossing K. ocelatus and the self-fertilising K. hermaphroditus identified that the diversity and community composition of the skin microbiome were strongly shaped by their environment but also by the species and host genetic diversity at different levels.his study also found first-time evidence of a relationship between microbiome and epigenetic diversity in these wild populations, suggesting that both mechanisms could be potential sources of additional variability for fish species with low genetic diversity.The global findings of this thesis on mangrove killifishes from different origins (laboratory and natural conditions) highlighted the importance of the interactions between genotype and environment in shaping fish microbiome composition and diversity.</abstract><type>E-Thesis</type><journal/><volume/><journalNumber/><paginationStart/><paginationEnd/><publisher/><placeOfPublication>Swansea University, Wales, UK</placeOfPublication><isbnPrint/><isbnElectronic/><issnPrint/><issnElectronic/><keywords>Genotype, environment, behaviour, microbiome</keywords><publishedDay>26</publishedDay><publishedMonth>7</publishedMonth><publishedYear>2024</publishedYear><publishedDate>2024-07-26</publishedDate><doi>10.23889/SUThesis.67285</doi><url/><notes>A selection of content is redacted or is partially redacted from this thesis to protect sensitive and personal information.</notes><college>COLLEGE NANME</college><CollegeCode>COLLEGE CODE</CollegeCode><institution>Swansea University</institution><supervisor>Consuegra, S., Garcia de Leaniz, C., and Uren Webster, T. M.</supervisor><degreelevel>Doctoral</degreelevel><degreename>Ph.D</degreename><degreesponsorsfunders>Commonwealth PhD Scholarship</degreesponsorsfunders><apcterm/><funders>Commonwealth PhD Scholarship</funders><projectreference/><lastEdited>2024-08-01T15:49:47.5587960</lastEdited><Created>2024-08-01T15:31:28.3921881</Created><path><level id="1">Faculty of Science and Engineering</level><level id="2">School of Biosciences, Geography and Physics - Biosciences</level></path><authors><author><firstname>ISHRAT</firstname><surname>ANKA</surname><order>1</order></author></authors><documents><document><filename>67285__31032__5820b381af2642729f7f394e3124b25e.pdf</filename><originalFilename>2024_Anka_I.final.67285.pdf</originalFilename><uploaded>2024-08-01T15:41:41.6994375</uploaded><type>Output</type><contentLength>8758141</contentLength><contentType>application/pdf</contentType><version>E-Thesis – open access</version><cronfaStatus>true</cronfaStatus><documentNotes>Copyright: The Author, Ishrat Zahan Anka, 2024</documentNotes><copyrightCorrect>true</copyrightCorrect><language>eng</language></document></documents><OutputDurs/></rfc1807>
spelling v2 67285 2024-08-01 Effect of early rearing conditions on the behaviour and microbiome of fish with low genetic diversity a463eddc946c95f3a3f15e3c13fcfb7d ISHRAT ANKA ISHRAT ANKA true false 2024-08-01 Fish performance is influenced by their genotype and environment. For populations with low genetic diversity, adaptation to environmental change can be compromised, but it has been suggested that the microbiome can act as an additional source of variability. Early rearing conditions can be particularly important for fish development and behaviour, due to their dependence on the environmental conditions. This thesis explored the interactions between fish genotype and rearing environment (diet and enrichment) on behaviour, metabolic rate and microbiome (gut, skin) using captive and wild populations of naturally inbred mangrove killifishes (Kryptolebias sp.), where the exploratory behaviour and closed respirometry were used to test the captive killifish and microbiome diversity analyses were performed in both captive and wild killifishes.Behavioural trials in self-fertilizing killifish (Kryptolebias marmoratus) indicated a significant effect of both genetic strain and rearing environment (environmental enrichment and diet) on fish activity measurements. Incubation time also had a substantial role on both fish behaviour and microbiome diversity. Gut-microbiome alpha diversity was shaped by strain, diet, and hatching time in K. marmoratus with interactions between diet and physical enrichment.An intergenerational influence of rearing environment on fish behaviour was detected in K.armoratus, where parental activity was found to influence offspring activity. Gut-microbial comparisons between parents and offspring identified Vibrionaceae as the dominant colonizers in laboratory reared K. marmoratus. A dominant effect of the rearing environment over strain on both microbiome composition and distribution was observed. The influence of the interaction between parental and own environments on microbiome alpha diversity in K. marmoratus offspring suggests long-term effects of the rearing environment on the fish (gut) microbiome.In the wild, results from the outcrossing K. ocelatus and the self-fertilising K. hermaphroditus identified that the diversity and community composition of the skin microbiome were strongly shaped by their environment but also by the species and host genetic diversity at different levels.his study also found first-time evidence of a relationship between microbiome and epigenetic diversity in these wild populations, suggesting that both mechanisms could be potential sources of additional variability for fish species with low genetic diversity.The global findings of this thesis on mangrove killifishes from different origins (laboratory and natural conditions) highlighted the importance of the interactions between genotype and environment in shaping fish microbiome composition and diversity. E-Thesis Swansea University, Wales, UK Genotype, environment, behaviour, microbiome 26 7 2024 2024-07-26 10.23889/SUThesis.67285 A selection of content is redacted or is partially redacted from this thesis to protect sensitive and personal information. COLLEGE NANME COLLEGE CODE Swansea University Consuegra, S., Garcia de Leaniz, C., and Uren Webster, T. M. Doctoral Ph.D Commonwealth PhD Scholarship Commonwealth PhD Scholarship 2024-08-01T15:49:47.5587960 2024-08-01T15:31:28.3921881 Faculty of Science and Engineering School of Biosciences, Geography and Physics - Biosciences ISHRAT ANKA 1 67285__31032__5820b381af2642729f7f394e3124b25e.pdf 2024_Anka_I.final.67285.pdf 2024-08-01T15:41:41.6994375 Output 8758141 application/pdf E-Thesis – open access true Copyright: The Author, Ishrat Zahan Anka, 2024 true eng
title Effect of early rearing conditions on the behaviour and microbiome of fish with low genetic diversity
spellingShingle Effect of early rearing conditions on the behaviour and microbiome of fish with low genetic diversity
ISHRAT ANKA
title_short Effect of early rearing conditions on the behaviour and microbiome of fish with low genetic diversity
title_full Effect of early rearing conditions on the behaviour and microbiome of fish with low genetic diversity
title_fullStr Effect of early rearing conditions on the behaviour and microbiome of fish with low genetic diversity
title_full_unstemmed Effect of early rearing conditions on the behaviour and microbiome of fish with low genetic diversity
title_sort Effect of early rearing conditions on the behaviour and microbiome of fish with low genetic diversity
author_id_str_mv a463eddc946c95f3a3f15e3c13fcfb7d
author_id_fullname_str_mv a463eddc946c95f3a3f15e3c13fcfb7d_***_ISHRAT ANKA
author ISHRAT ANKA
author2 ISHRAT ANKA
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publishDate 2024
institution Swansea University
doi_str_mv 10.23889/SUThesis.67285
college_str Faculty of Science and Engineering
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hierarchy_top_id facultyofscienceandengineering
hierarchy_top_title Faculty of Science and Engineering
hierarchy_parent_id facultyofscienceandengineering
hierarchy_parent_title Faculty of Science and Engineering
department_str School of Biosciences, Geography and Physics - Biosciences{{{_:::_}}}Faculty of Science and Engineering{{{_:::_}}}School of Biosciences, Geography and Physics - Biosciences
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description Fish performance is influenced by their genotype and environment. For populations with low genetic diversity, adaptation to environmental change can be compromised, but it has been suggested that the microbiome can act as an additional source of variability. Early rearing conditions can be particularly important for fish development and behaviour, due to their dependence on the environmental conditions. This thesis explored the interactions between fish genotype and rearing environment (diet and enrichment) on behaviour, metabolic rate and microbiome (gut, skin) using captive and wild populations of naturally inbred mangrove killifishes (Kryptolebias sp.), where the exploratory behaviour and closed respirometry were used to test the captive killifish and microbiome diversity analyses were performed in both captive and wild killifishes.Behavioural trials in self-fertilizing killifish (Kryptolebias marmoratus) indicated a significant effect of both genetic strain and rearing environment (environmental enrichment and diet) on fish activity measurements. Incubation time also had a substantial role on both fish behaviour and microbiome diversity. Gut-microbiome alpha diversity was shaped by strain, diet, and hatching time in K. marmoratus with interactions between diet and physical enrichment.An intergenerational influence of rearing environment on fish behaviour was detected in K.armoratus, where parental activity was found to influence offspring activity. Gut-microbial comparisons between parents and offspring identified Vibrionaceae as the dominant colonizers in laboratory reared K. marmoratus. A dominant effect of the rearing environment over strain on both microbiome composition and distribution was observed. The influence of the interaction between parental and own environments on microbiome alpha diversity in K. marmoratus offspring suggests long-term effects of the rearing environment on the fish (gut) microbiome.In the wild, results from the outcrossing K. ocelatus and the self-fertilising K. hermaphroditus identified that the diversity and community composition of the skin microbiome were strongly shaped by their environment but also by the species and host genetic diversity at different levels.his study also found first-time evidence of a relationship between microbiome and epigenetic diversity in these wild populations, suggesting that both mechanisms could be potential sources of additional variability for fish species with low genetic diversity.The global findings of this thesis on mangrove killifishes from different origins (laboratory and natural conditions) highlighted the importance of the interactions between genotype and environment in shaping fish microbiome composition and diversity.
published_date 2024-07-26T15:49:47Z
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