Exploring the Role of Fibrin Gels in Enhancing Cell Migration for Vasculature Formation

Moura, Joana A.; Barlow, Hugh J.; Doak, Shareen; Hawkins, Karl; Muller, Iris; Clift, Martin

doi:10.3390/jfb15090265

Journal article 56 views

Exploring the Role of Fibrin Gels in Enhancing Cell Migration for Vasculature Formation

Joana A. Moura

, Hugh J. Barlow, Shareen Doak

, Karl Hawkins

, Iris Muller, Martin Clift

Journal of Functional Biomaterials, Volume: 15, Issue: 9, Start page: 265

Swansea University Authors: Shareen Doak , Karl Hawkins , Martin Clift

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DOI (Published version): 10.3390/jfb15090265

Abstract

A hallmark of angiogenesis is the sprouting of endothelial cells. To replicate this event in vitro, biomaterial approaches can play an essential role in promoting cell migration. To study the ca-pacity of a scaffold of fibrin (fibrinogen:thrombin mix) to support the movement of the endothelial cells...

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Published in:	Journal of Functional Biomaterials
ISSN:	2079-4983
Published:	MDPI AG 2024
Online Access:	Check full text
URI:	https://cronfa.swan.ac.uk/Record/cronfa67608
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first_indexed	2024-09-09T07:57:33Z
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spelling	v2 67608 2024-09-09 Exploring the Role of Fibrin Gels in Enhancing Cell Migration for Vasculature Formation 8f70286908f67238a527a98cbf66d387 0000-0002-6753-1987 Shareen Doak Shareen Doak true false 77c39404a9a98c6e2283d84815cba053 0000-0003-0174-4151 Karl Hawkins Karl Hawkins true false 71bf49b157691e541950f5c3f49c9169 0000-0001-6133-3368 Martin Clift Martin Clift true false 2024-09-09 MEDS A hallmark of angiogenesis is the sprouting of endothelial cells. To replicate this event in vitro, biomaterial approaches can play an essential role in promoting cell migration. To study the ca-pacity of a scaffold of fibrin (fibrinogen:thrombin mix) to support the movement of the endothelial cells, the migration area of spheroids formed with the HULEC cell line was measured. The cells were first allowed to form a spheroid using the hanging drop technique before being encapsulated in the fibrin gel. The cells’ migration area was then measured after two days of embedding in the fibrin gel. Various conditions affecting fibrin gel polymerization, such as different concentrations of fibrinogen and thrombin, were evaluated alongside rheology, porosity, and fiber thickness analysis to understand how these factors influenced cell behavior within the composite bio-material. Data point toward thrombin’s role in governing fibrin gel polymerization; higher con-centrations result in less rigid gels (loss tangent between 0.07 and 0.034) and increased cell mi-gration (maximum concentration tested: 5 U/mL). The herein presented method allows for a more precise determination of the crosslinking conditions of fibrin gel that can be used to stimulate an-giogenic sprouting. Journal Article Journal of Functional Biomaterials 15 9 265 MDPI AG 2079-4983 cell migration; vasculature; sprouting; endothelial cells; biomaterial; fibrin 12 9 2024 2024-09-12 10.3390/jfb15090265 http://dx.doi.org/10.3390/jfb15090265 COLLEGE NANME Medical School COLLEGE CODE MEDS Swansea University Other This project was funded by Unilever (Project #: MA-2016-02191N). MA-2016-02191N 2024-09-25T13:15:38.1519822 2024-09-09T08:51:47.1883570 Faculty of Medicine, Health and Life Sciences Swansea University Medical School - Biomedical Science Joana A. Moura 0009-0008-9778-8442 1 Hugh J. Barlow 2 Shareen Doak 0000-0002-6753-1987 3 Karl Hawkins 0000-0003-0174-4151 4 Iris Muller 5 Martin Clift 0000-0001-6133-3368 6
title	Exploring the Role of Fibrin Gels in Enhancing Cell Migration for Vasculature Formation
spellingShingle	Exploring the Role of Fibrin Gels in Enhancing Cell Migration for Vasculature Formation Shareen Doak Karl Hawkins Martin Clift
title_short	Exploring the Role of Fibrin Gels in Enhancing Cell Migration for Vasculature Formation
title_full	Exploring the Role of Fibrin Gels in Enhancing Cell Migration for Vasculature Formation
title_fullStr	Exploring the Role of Fibrin Gels in Enhancing Cell Migration for Vasculature Formation
title_full_unstemmed	Exploring the Role of Fibrin Gels in Enhancing Cell Migration for Vasculature Formation
title_sort	Exploring the Role of Fibrin Gels in Enhancing Cell Migration for Vasculature Formation
author_id_str_mv	8f70286908f67238a527a98cbf66d387 77c39404a9a98c6e2283d84815cba053 71bf49b157691e541950f5c3f49c9169
author_id_fullname_str_mv	8f70286908f67238a527a98cbf66d387_*_Shareen Doak 77c39404a9a98c6e2283d84815cba053__Karl Hawkins 71bf49b157691e541950f5c3f49c9169_**_Martin Clift
author	Shareen Doak Karl Hawkins Martin Clift
author2	Joana A. Moura Hugh J. Barlow Shareen Doak Karl Hawkins Iris Muller Martin Clift
format	Journal article
container_title	Journal of Functional Biomaterials
container_volume	15
container_issue	9
container_start_page	265
publishDate	2024
institution	Swansea University
issn	2079-4983
doi_str_mv	10.3390/jfb15090265
publisher	MDPI AG
college_str	Faculty of Medicine, Health and Life Sciences
hierarchytype
hierarchy_top_id	facultyofmedicinehealthandlifesciences
hierarchy_top_title	Faculty of Medicine, Health and Life Sciences
hierarchy_parent_id	facultyofmedicinehealthandlifesciences
hierarchy_parent_title	Faculty of Medicine, Health and Life Sciences
department_str	Swansea University Medical School - Biomedical Science{{{_:::_}}}Faculty of Medicine, Health and Life Sciences{{{_:::_}}}Swansea University Medical School - Biomedical Science
url	http://dx.doi.org/10.3390/jfb15090265
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description	A hallmark of angiogenesis is the sprouting of endothelial cells. To replicate this event in vitro, biomaterial approaches can play an essential role in promoting cell migration. To study the ca-pacity of a scaffold of fibrin (fibrinogen:thrombin mix) to support the movement of the endothelial cells, the migration area of spheroids formed with the HULEC cell line was measured. The cells were first allowed to form a spheroid using the hanging drop technique before being encapsulated in the fibrin gel. The cells’ migration area was then measured after two days of embedding in the fibrin gel. Various conditions affecting fibrin gel polymerization, such as different concentrations of fibrinogen and thrombin, were evaluated alongside rheology, porosity, and fiber thickness analysis to understand how these factors influenced cell behavior within the composite bio-material. Data point toward thrombin’s role in governing fibrin gel polymerization; higher con-centrations result in less rigid gels (loss tangent between 0.07 and 0.034) and increased cell mi-gration (maximum concentration tested: 5 U/mL). The herein presented method allows for a more precise determination of the crosslinking conditions of fibrin gel that can be used to stimulate an-giogenic sprouting.
published_date	2024-09-12T13:15:37Z
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Exploring the Role of Fibrin Gels in Enhancing Cell Migration for Vasculature Formation

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