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Exploring the Role of Fibrin Gels in Enhancing Cell Migration for Vasculature Formation

Joana A. Moura Orcid Logo, Hugh J. Barlow, shareen Doak, Karl Hawkins Orcid Logo, Iris Muller, Martin Clift Orcid Logo

Journal of Functional Biomaterials, Volume: 15, Issue: 9, Start page: 265

Swansea University Authors: shareen Doak, Karl Hawkins Orcid Logo, Martin Clift Orcid Logo

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DOI (Published version): 10.3390/jfb15090265

Abstract

A hallmark of angiogenesis is the sprouting of endothelial cells. To replicate this event in vitro, biomaterial approaches can play an essential role in promoting cell migration. To study the ca-pacity of a scaffold of fibrin (fibrinogen:thrombin mix) to support the movement of the endothelial cells...

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Published in: Journal of Functional Biomaterials
ISSN: 2079-4983
Published: MDPI AG 2024
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URI: https://cronfa.swan.ac.uk/Record/cronfa67608
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last_indexed 2024-11-25T14:20:29Z
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spelling 2024-10-25T12:24:01.7340377 v2 67608 2024-09-09 Exploring the Role of Fibrin Gels in Enhancing Cell Migration for Vasculature Formation 8f70286908f67238a527a98cbf66d387 shareen Doak shareen Doak true false 77c39404a9a98c6e2283d84815cba053 0000-0003-0174-4151 Karl Hawkins Karl Hawkins true false 71bf49b157691e541950f5c3f49c9169 0000-0001-6133-3368 Martin Clift Martin Clift true false 2024-09-09 A hallmark of angiogenesis is the sprouting of endothelial cells. To replicate this event in vitro, biomaterial approaches can play an essential role in promoting cell migration. To study the ca-pacity of a scaffold of fibrin (fibrinogen:thrombin mix) to support the movement of the endothelial cells, the migration area of spheroids formed with the HULEC cell line was measured. The cells were first allowed to form a spheroid using the hanging drop technique before being encapsulated in the fibrin gel. The cells’ migration area was then measured after two days of embedding in the fibrin gel. Various conditions affecting fibrin gel polymerization, such as different concentrations of fibrinogen and thrombin, were evaluated alongside rheology, porosity, and fiber thickness analysis to understand how these factors influenced cell behavior within the composite bio-material. Data point toward thrombin’s role in governing fibrin gel polymerization; higher con-centrations result in less rigid gels (loss tangent between 0.07 and 0.034) and increased cell mi-gration (maximum concentration tested: 5 U/mL). The herein presented method allows for a more precise determination of the crosslinking conditions of fibrin gel that can be used to stimulate an-giogenic sprouting. Journal Article Journal of Functional Biomaterials 15 9 265 MDPI AG 2079-4983 cell migration; vasculature; sprouting; endothelial cells; biomaterial; fibrin 12 9 2024 2024-09-12 10.3390/jfb15090265 COLLEGE NANME COLLEGE CODE Swansea University Other This project was funded by Unilever (Project #: MA-2016-02191N). 2024-10-25T12:24:01.7340377 2024-09-09T08:51:47.1883570 Faculty of Medicine, Health and Life Sciences Swansea University Medical School - Biomedical Science Joana A. Moura 0009-0008-9778-8442 1 Hugh J. Barlow 2 shareen Doak 3 Karl Hawkins 0000-0003-0174-4151 4 Iris Muller 5 Martin Clift 0000-0001-6133-3368 6 67608__32719__135293ebe7784a7f9f7412ebd66dd975.pdf 67608.VoR.pdf 2024-10-25T12:22:39.8979916 Output 9383815 application/pdf Version of Record true © 2024 by the authors. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license. true eng https://creativecommons.org/licenses/by/4.0/
title Exploring the Role of Fibrin Gels in Enhancing Cell Migration for Vasculature Formation
spellingShingle Exploring the Role of Fibrin Gels in Enhancing Cell Migration for Vasculature Formation
shareen Doak
Karl Hawkins
Martin Clift
title_short Exploring the Role of Fibrin Gels in Enhancing Cell Migration for Vasculature Formation
title_full Exploring the Role of Fibrin Gels in Enhancing Cell Migration for Vasculature Formation
title_fullStr Exploring the Role of Fibrin Gels in Enhancing Cell Migration for Vasculature Formation
title_full_unstemmed Exploring the Role of Fibrin Gels in Enhancing Cell Migration for Vasculature Formation
title_sort Exploring the Role of Fibrin Gels in Enhancing Cell Migration for Vasculature Formation
author_id_str_mv 8f70286908f67238a527a98cbf66d387
77c39404a9a98c6e2283d84815cba053
71bf49b157691e541950f5c3f49c9169
author_id_fullname_str_mv 8f70286908f67238a527a98cbf66d387_***_shareen Doak
77c39404a9a98c6e2283d84815cba053_***_Karl Hawkins
71bf49b157691e541950f5c3f49c9169_***_Martin Clift
author shareen Doak
Karl Hawkins
Martin Clift
author2 Joana A. Moura
Hugh J. Barlow
shareen Doak
Karl Hawkins
Iris Muller
Martin Clift
format Journal article
container_title Journal of Functional Biomaterials
container_volume 15
container_issue 9
container_start_page 265
publishDate 2024
institution Swansea University
issn 2079-4983
doi_str_mv 10.3390/jfb15090265
publisher MDPI AG
college_str Faculty of Medicine, Health and Life Sciences
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hierarchy_top_id facultyofmedicinehealthandlifesciences
hierarchy_top_title Faculty of Medicine, Health and Life Sciences
hierarchy_parent_id facultyofmedicinehealthandlifesciences
hierarchy_parent_title Faculty of Medicine, Health and Life Sciences
department_str Swansea University Medical School - Biomedical Science{{{_:::_}}}Faculty of Medicine, Health and Life Sciences{{{_:::_}}}Swansea University Medical School - Biomedical Science
document_store_str 1
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description A hallmark of angiogenesis is the sprouting of endothelial cells. To replicate this event in vitro, biomaterial approaches can play an essential role in promoting cell migration. To study the ca-pacity of a scaffold of fibrin (fibrinogen:thrombin mix) to support the movement of the endothelial cells, the migration area of spheroids formed with the HULEC cell line was measured. The cells were first allowed to form a spheroid using the hanging drop technique before being encapsulated in the fibrin gel. The cells’ migration area was then measured after two days of embedding in the fibrin gel. Various conditions affecting fibrin gel polymerization, such as different concentrations of fibrinogen and thrombin, were evaluated alongside rheology, porosity, and fiber thickness analysis to understand how these factors influenced cell behavior within the composite bio-material. Data point toward thrombin’s role in governing fibrin gel polymerization; higher con-centrations result in less rigid gels (loss tangent between 0.07 and 0.034) and increased cell mi-gration (maximum concentration tested: 5 U/mL). The herein presented method allows for a more precise determination of the crosslinking conditions of fibrin gel that can be used to stimulate an-giogenic sprouting.
published_date 2024-09-12T05:22:08Z
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