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Transcriptomic and protein analysis of Trametes versicolor interacting with a Hypholoma fasciculare mycelium foraging in soil

G. Attrill, L. Boddy, E. Dudley, Bethany Greenfield, Dan Eastwood Orcid Logo

Fungal Ecology, Volume: 72

Swansea University Authors: Bethany Greenfield, Dan Eastwood Orcid Logo

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DOI (Published version): 10.1016/j.funeco.2024.101385

Abstract

The decomposition of large woody material is an important process in forest carbon cycling and nutrient release. Cord-forming saprotrophic basidiomycete fungi create non-resource limited mycelial networks between decomposing branches, logs and tree stumps on the forest floor where colonisation of ne...

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Published in: Fungal Ecology
ISSN: 1754-5048 1878-0083
Published: Elsevier BV 2024
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URI: https://cronfa.swan.ac.uk/Record/cronfa67855
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spelling v2 67855 2024-09-30 Transcriptomic and protein analysis of Trametes versicolor interacting with a Hypholoma fasciculare mycelium foraging in soil 6b2a6e5491bc29cc6352a83f25ab0349 Bethany Greenfield Bethany Greenfield true false 4982f3fa83886c0362e2bb43ce1c027f 0000-0002-7015-0739 Dan Eastwood Dan Eastwood true false 2024-09-30 NRW The decomposition of large woody material is an important process in forest carbon cycling and nutrient release. Cord-forming saprotrophic basidiomycete fungi create non-resource limited mycelial networks between decomposing branches, logs and tree stumps on the forest floor where colonisation of new resources is often associated with the replacement of incumbent decay communities. To date, antagonism experiments have mostly placed competing fungi in direct contact, while in nature cord-forming saprobes encounter colonised wood as mycelia in a network. Transcriptomic and peptide analyses were conducted on soil-based microcosms were foraging cord-forming Hypholoma fasciculare encountered a wood block colonised by Trametes versicolor. Protein turnover featured strongly for both species and genes putatively involved in secondary metabolite production were identified. H. fasciculare demonstrated an exploitative profile with increased transcription of genes associated with carbohydrate metabolism and RNA and ribosome processing. T. versicolor showed a shift in signalling, energy generation and amino acid metabolism. By identifying genes and proteins putatively involved in this fungal interaction, this work may help guide the discovery of bioactive molecules and mechanisms underpinning community succession. Journal Article Fungal Ecology 72 Elsevier BV 1754-5048 1878-0083 1 12 2024 2024-12-01 10.1016/j.funeco.2024.101385 COLLEGE NANME Natural Resources Wales COLLEGE CODE NRW Swansea University SU Library paid the OA fee (TA Institutional Deal) The research described in this manuscript was funded by UK Natural Environment Research Council award reference 1319716. We also thank Jennifer Hiscox and Melanie Savory for advice with experimental design. 2024-09-30T11:48:05.4370624 2024-09-30T11:21:29.6037031 Faculty of Science and Engineering School of Biosciences, Geography and Physics - Biosciences G. Attrill 1 L. Boddy 2 E. Dudley 3 Bethany Greenfield 4 Dan Eastwood 0000-0002-7015-0739 5 67855__31472__e050b2a694844141ac331cf8f01e8b0d.pdf 67855.VOR.pdf 2024-09-30T11:30:26.8364793 Output 15890793 application/pdf Version of Record true © 2024 The Author(s). This is an open access article under the CC BY license. true eng http://creativecommons.org/licenses/by/4.0/
title Transcriptomic and protein analysis of Trametes versicolor interacting with a Hypholoma fasciculare mycelium foraging in soil
spellingShingle Transcriptomic and protein analysis of Trametes versicolor interacting with a Hypholoma fasciculare mycelium foraging in soil
Bethany Greenfield
Dan Eastwood
title_short Transcriptomic and protein analysis of Trametes versicolor interacting with a Hypholoma fasciculare mycelium foraging in soil
title_full Transcriptomic and protein analysis of Trametes versicolor interacting with a Hypholoma fasciculare mycelium foraging in soil
title_fullStr Transcriptomic and protein analysis of Trametes versicolor interacting with a Hypholoma fasciculare mycelium foraging in soil
title_full_unstemmed Transcriptomic and protein analysis of Trametes versicolor interacting with a Hypholoma fasciculare mycelium foraging in soil
title_sort Transcriptomic and protein analysis of Trametes versicolor interacting with a Hypholoma fasciculare mycelium foraging in soil
author_id_str_mv 6b2a6e5491bc29cc6352a83f25ab0349
4982f3fa83886c0362e2bb43ce1c027f
author_id_fullname_str_mv 6b2a6e5491bc29cc6352a83f25ab0349_***_Bethany Greenfield
4982f3fa83886c0362e2bb43ce1c027f_***_Dan Eastwood
author Bethany Greenfield
Dan Eastwood
author2 G. Attrill
L. Boddy
E. Dudley
Bethany Greenfield
Dan Eastwood
format Journal article
container_title Fungal Ecology
container_volume 72
publishDate 2024
institution Swansea University
issn 1754-5048
1878-0083
doi_str_mv 10.1016/j.funeco.2024.101385
publisher Elsevier BV
college_str Faculty of Science and Engineering
hierarchytype
hierarchy_top_id facultyofscienceandengineering
hierarchy_top_title Faculty of Science and Engineering
hierarchy_parent_id facultyofscienceandengineering
hierarchy_parent_title Faculty of Science and Engineering
department_str School of Biosciences, Geography and Physics - Biosciences{{{_:::_}}}Faculty of Science and Engineering{{{_:::_}}}School of Biosciences, Geography and Physics - Biosciences
document_store_str 1
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description The decomposition of large woody material is an important process in forest carbon cycling and nutrient release. Cord-forming saprotrophic basidiomycete fungi create non-resource limited mycelial networks between decomposing branches, logs and tree stumps on the forest floor where colonisation of new resources is often associated with the replacement of incumbent decay communities. To date, antagonism experiments have mostly placed competing fungi in direct contact, while in nature cord-forming saprobes encounter colonised wood as mycelia in a network. Transcriptomic and peptide analyses were conducted on soil-based microcosms were foraging cord-forming Hypholoma fasciculare encountered a wood block colonised by Trametes versicolor. Protein turnover featured strongly for both species and genes putatively involved in secondary metabolite production were identified. H. fasciculare demonstrated an exploitative profile with increased transcription of genes associated with carbohydrate metabolism and RNA and ribosome processing. T. versicolor showed a shift in signalling, energy generation and amino acid metabolism. By identifying genes and proteins putatively involved in this fungal interaction, this work may help guide the discovery of bioactive molecules and mechanisms underpinning community succession.
published_date 2024-12-01T11:48:04Z
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