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The ability of the Lab4 probiotic consortium to impact upon the functionality of serum deprived human keratinocytes in vitro

Sophie E. Thomas, Joshua Kerry-Smith, Susan F. Plummer, Jack P. Bate, Daniel A. John, Evie Lawrence, Lydia Powell Orcid Logo, Jordanna Dally, Ryan Moseley, Daryn R. Michael

Frontiers in Microbiomes, Volume: 3, Start page: 1488650

Swansea University Author: Lydia Powell Orcid Logo

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    © 2024 Thomas, Kerry-Smith, Plummer, Bate, John, Lawrence, Powell, Dally, Moseley and Michael. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY).

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DOI (Published version): 10.3389/frmbi.2024.1488650

Abstract

Introduction: Dysfunction of keratinocytes contributes to a weakened skin barrier and impaired wound healing capability. Evidence suggests that probiotic supplementation can lead to improved skin function in vitro and in vivo. The Lab4 probiotic consortium comprises of two strains of Lactobacillus s...

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Published in: Frontiers in Microbiomes
ISSN: 2813-4338
Published: Frontiers Media SA 2024
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URI: https://cronfa.swan.ac.uk/Record/cronfa68318
Abstract: Introduction: Dysfunction of keratinocytes contributes to a weakened skin barrier and impaired wound healing capability. Evidence suggests that probiotic supplementation can lead to improved skin function in vitro and in vivo. The Lab4 probiotic consortium comprises of two strains of Lactobacillus species and two strains of Bifidobacterium species. Methods: Using serum deprived conditions to impair the functionality of immortalized human HaCaT keratinocytes, this study aimed to assess the impact of metabolites derived from the Lab4 probiotic consortium on keratinocyte function. Results: A significant improvement in HaCaT metabolic activity and lower apoptotic activity was observed in tandem with a reduction in Caspase-3 gene expression and a lower Bax/Bcl2 ratio following the addition of Lab4. The probiotic also supported barrier integrity which was better maintained with a significant increase in Filaggrin gene expression. In damaged keratinocytes, Lab4 enhanced rates of re-epithelialization, which were associated with significantly increased gene expression of MMP-1 and enhanced secretion of IL-6 and IL-8. Discussion: These results suggest that the Lab4 probiotic consortium may have the ability to benefit the functionality of skin.
Keywords: keratinocyte, serum deprivation, probiotic, gut-skin axis, Lab4, conditioned media
College: Faculty of Medicine, Health and Life Sciences
Funders: This study was funded by Cultech Ltd.
Start Page: 1488650

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