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Graded porous scaffold mediates internal fluidic environment for 3D in vitro mechanobiology

Christopher Wright Orcid Logo, Yi Xiong Orcid Logo, Francesco Del Giudice Orcid Logo, Feihu Zhao Orcid Logo

Computers in Biology and Medicine, Volume: 186, Start page: 109674

Swansea University Authors: Christopher Wright Orcid Logo, Francesco Del Giudice Orcid Logo, Feihu Zhao Orcid Logo

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DOI (Published version): 10.1016/j.compbiomed.2025.109674

Abstract

Most cell types are mechanosensitive, their activities such as differentiation, proliferation and apoptosis, can be influenced by the mechanical environment through mechanical stimulation. In three dimensional (3D) mechanobiological in vitro studies, the porous structure of scaffold controls the loc...

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Published in: Computers in Biology and Medicine
ISSN: 0010-4825 1879-0534
Published: Elsevier BV 2025
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URI: https://cronfa.swan.ac.uk/Record/cronfa68692
first_indexed 2025-01-14T14:39:21Z
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spelling 2025-01-14T10:01:47.2387115 v2 68692 2025-01-14 Graded porous scaffold mediates internal fluidic environment for 3D in vitro mechanobiology 235e125ac3463e2ee7fc98604bf879ce 0000-0003-2375-8159 Christopher Wright Christopher Wright true false 742d483071479b44d7888e16166b1309 0000-0002-9414-6937 Francesco Del Giudice Francesco Del Giudice true false 1c6e79b6edd08c88a8d17a241cd78630 0000-0003-0515-6808 Feihu Zhao Feihu Zhao true false 2025-01-14 EAAS Most cell types are mechanosensitive, their activities such as differentiation, proliferation and apoptosis, can be influenced by the mechanical environment through mechanical stimulation. In three dimensional (3D) mechanobiological in vitro studies, the porous structure of scaffold controls the local mechanical environment that applied to cells. Many previous studies have focused on the topological design of homogeneous scaffold struts. However, the impact of scaffold inhomogeneity on the mechanical environment, which is essential in mechanobiological application (e.g. for multi-cells co-culture), remains elusive. In this study, we use a computational fluid dynamics (CFD) approach together with data analysis to study the influence of a porosity gradient (10 %–30 % porosity difference) on the local and global mechanical environment (wall shear stress - WSS) within the commonly used structures of triple periodic minimal surfaces (TPMS). In addition, the anisotropy of internal WSS and scaffold permeability caused by the porosity gradient is investigated. It is found that the influence of anisotropy on the average WSS and permeability is up to 11 % and 31 %, respectively. These results, as theoretical references will be useful to tissue engineers and mechanobiologists for scaffold design and in vitro experiment planning such as integrated use of graded scaffold and bioreactors for specific cell types. Journal Article Computers in Biology and Medicine 186 109674 Elsevier BV 0010-4825 1879-0534 Graded scaffolds; Wall shear stress; Permeability; Anisotropic property 1 3 2025 2025-03-01 10.1016/j.compbiomed.2025.109674 COLLEGE NANME Engineering and Applied Sciences School COLLEGE CODE EAAS Swansea University SU Library paid the OA fee (TA Institutional Deal) This study was supported by the Erasmus+ Programme from European Union (scholarship reference: 2022-IT02-KA131-HED-000059894). 2025-01-14T10:01:47.2387115 2025-01-14T09:55:23.7899208 Faculty of Science and Engineering School of Engineering and Applied Sciences - Biomedical Engineering Christopher Wright 0000-0003-2375-8159 1 Yi Xiong 0000-0002-0184-8607 2 Francesco Del Giudice 0000-0002-9414-6937 3 Feihu Zhao 0000-0003-0515-6808 4
title Graded porous scaffold mediates internal fluidic environment for 3D in vitro mechanobiology
spellingShingle Graded porous scaffold mediates internal fluidic environment for 3D in vitro mechanobiology
Christopher Wright
Francesco Del Giudice
Feihu Zhao
title_short Graded porous scaffold mediates internal fluidic environment for 3D in vitro mechanobiology
title_full Graded porous scaffold mediates internal fluidic environment for 3D in vitro mechanobiology
title_fullStr Graded porous scaffold mediates internal fluidic environment for 3D in vitro mechanobiology
title_full_unstemmed Graded porous scaffold mediates internal fluidic environment for 3D in vitro mechanobiology
title_sort Graded porous scaffold mediates internal fluidic environment for 3D in vitro mechanobiology
author_id_str_mv 235e125ac3463e2ee7fc98604bf879ce
742d483071479b44d7888e16166b1309
1c6e79b6edd08c88a8d17a241cd78630
author_id_fullname_str_mv 235e125ac3463e2ee7fc98604bf879ce_***_Christopher Wright
742d483071479b44d7888e16166b1309_***_Francesco Del Giudice
1c6e79b6edd08c88a8d17a241cd78630_***_Feihu Zhao
author Christopher Wright
Francesco Del Giudice
Feihu Zhao
author2 Christopher Wright
Yi Xiong
Francesco Del Giudice
Feihu Zhao
format Journal article
container_title Computers in Biology and Medicine
container_volume 186
container_start_page 109674
publishDate 2025
institution Swansea University
issn 0010-4825
1879-0534
doi_str_mv 10.1016/j.compbiomed.2025.109674
publisher Elsevier BV
college_str Faculty of Science and Engineering
hierarchytype
hierarchy_top_id facultyofscienceandengineering
hierarchy_top_title Faculty of Science and Engineering
hierarchy_parent_id facultyofscienceandengineering
hierarchy_parent_title Faculty of Science and Engineering
department_str School of Engineering and Applied Sciences - Biomedical Engineering{{{_:::_}}}Faculty of Science and Engineering{{{_:::_}}}School of Engineering and Applied Sciences - Biomedical Engineering
document_store_str 0
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description Most cell types are mechanosensitive, their activities such as differentiation, proliferation and apoptosis, can be influenced by the mechanical environment through mechanical stimulation. In three dimensional (3D) mechanobiological in vitro studies, the porous structure of scaffold controls the local mechanical environment that applied to cells. Many previous studies have focused on the topological design of homogeneous scaffold struts. However, the impact of scaffold inhomogeneity on the mechanical environment, which is essential in mechanobiological application (e.g. for multi-cells co-culture), remains elusive. In this study, we use a computational fluid dynamics (CFD) approach together with data analysis to study the influence of a porosity gradient (10 %–30 % porosity difference) on the local and global mechanical environment (wall shear stress - WSS) within the commonly used structures of triple periodic minimal surfaces (TPMS). In addition, the anisotropy of internal WSS and scaffold permeability caused by the porosity gradient is investigated. It is found that the influence of anisotropy on the average WSS and permeability is up to 11 % and 31 %, respectively. These results, as theoretical references will be useful to tissue engineers and mechanobiologists for scaffold design and in vitro experiment planning such as integrated use of graded scaffold and bioreactors for specific cell types.
published_date 2025-03-01T20:37:14Z
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