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Space-time Fourier ptychography for in vivo quantitative phase imaging

Ming Sun Orcid Logo, Kunyi Wang Orcid Logo, Yogeshwar Nath Mishra Orcid Logo, Simeng Qiu Orcid Logo, Wolfgang Heidrich Orcid Logo

Optica, Volume: 11, Issue: 9, Start page: 1250

Swansea University Author: Simeng Qiu Orcid Logo

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DOI (Published version): 10.1364/optica.531646

Abstract

Quantitative phase imaging of living biological specimens is challenging due to their continuous movement and complex behavior. Here, we introduce space-time Fourier ptychography (ST-FP), which combines a fast Fourier ptychography (FP) model based on compressive sensing with space-time motion priors...

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Published in: Optica
ISSN: 2334-2536
Published: Optica Publishing Group 2024
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URI: https://cronfa.swan.ac.uk/Record/cronfa69394
first_indexed 2025-05-01T13:11:57Z
last_indexed 2025-06-19T10:46:26Z
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spelling 2025-06-18T13:03:19.0791913 v2 69394 2025-05-01 Space-time Fourier ptychography for in vivo quantitative phase imaging d23fecb1c7e3005f0d349980539c2f95 0000-0002-0809-0093 Simeng Qiu Simeng Qiu true false 2025-05-01 MACS Quantitative phase imaging of living biological specimens is challenging due to their continuous movement and complex behavior. Here, we introduce space-time Fourier ptychography (ST-FP), which combines a fast Fourier ptychography (FP) model based on compressive sensing with space-time motion priors for joint reconstruction of quantitative phase, intensity, and motion fields across consecutive frames. Using the same input data as compressive sensing FP, ST-FP increases the space-bandwidth-time product of the reconstructed complex image sequence while leveraging redundant temporal information to achieve robust reconstruction performance. The efficacy of this approach is demonstrated across various applications, particularly in observing living microorganisms undergoing rapid morphological changes and reconstructing amplitude and phase targets in motion. The improved phase retrieval capability of ST-FP enables digital refocusing, facilitating comprehensive three-dimensional analysis of microorganisms. This advancement paves the way for enhanced visualization of cellular processes, developmental biology studies, and investigations into life mechanics at the microscopic level. Journal Article Optica 11 9 1250 Optica Publishing Group 2334-2536 3 9 2024 2024-09-03 10.1364/optica.531646 COLLEGE NANME Mathematics and Computer Science School COLLEGE CODE MACS Swansea University King Abdullah University of Science and Technology (Individual Baseline Research Fund). 2025-06-18T13:03:19.0791913 2025-05-01T14:08:20.2900970 Faculty of Science and Engineering School of Mathematics and Computer Science - Computer Science Ming Sun 0000-0002-5056-3800 1 Kunyi Wang 0009-0007-2832-0574 2 Yogeshwar Nath Mishra 0000-0003-2063-2200 3 Simeng Qiu 0000-0002-0809-0093 4 Wolfgang Heidrich 0000-0002-4227-8508 5 69394__34153__9bf8b4415d10492286c4e0862d89ef79.pdf 69394.pdf 2025-05-01T14:11:48.6894728 Output 3739451 application/pdf Version of Record true true eng
title Space-time Fourier ptychography for in vivo quantitative phase imaging
spellingShingle Space-time Fourier ptychography for in vivo quantitative phase imaging
Simeng Qiu
title_short Space-time Fourier ptychography for in vivo quantitative phase imaging
title_full Space-time Fourier ptychography for in vivo quantitative phase imaging
title_fullStr Space-time Fourier ptychography for in vivo quantitative phase imaging
title_full_unstemmed Space-time Fourier ptychography for in vivo quantitative phase imaging
title_sort Space-time Fourier ptychography for in vivo quantitative phase imaging
author_id_str_mv d23fecb1c7e3005f0d349980539c2f95
author_id_fullname_str_mv d23fecb1c7e3005f0d349980539c2f95_***_Simeng Qiu
author Simeng Qiu
author2 Ming Sun
Kunyi Wang
Yogeshwar Nath Mishra
Simeng Qiu
Wolfgang Heidrich
format Journal article
container_title Optica
container_volume 11
container_issue 9
container_start_page 1250
publishDate 2024
institution Swansea University
issn 2334-2536
doi_str_mv 10.1364/optica.531646
publisher Optica Publishing Group
college_str Faculty of Science and Engineering
hierarchytype
hierarchy_top_id facultyofscienceandengineering
hierarchy_top_title Faculty of Science and Engineering
hierarchy_parent_id facultyofscienceandengineering
hierarchy_parent_title Faculty of Science and Engineering
department_str School of Mathematics and Computer Science - Computer Science{{{_:::_}}}Faculty of Science and Engineering{{{_:::_}}}School of Mathematics and Computer Science - Computer Science
document_store_str 1
active_str 0
description Quantitative phase imaging of living biological specimens is challenging due to their continuous movement and complex behavior. Here, we introduce space-time Fourier ptychography (ST-FP), which combines a fast Fourier ptychography (FP) model based on compressive sensing with space-time motion priors for joint reconstruction of quantitative phase, intensity, and motion fields across consecutive frames. Using the same input data as compressive sensing FP, ST-FP increases the space-bandwidth-time product of the reconstructed complex image sequence while leveraging redundant temporal information to achieve robust reconstruction performance. The efficacy of this approach is demonstrated across various applications, particularly in observing living microorganisms undergoing rapid morphological changes and reconstructing amplitude and phase targets in motion. The improved phase retrieval capability of ST-FP enables digital refocusing, facilitating comprehensive three-dimensional analysis of microorganisms. This advancement paves the way for enhanced visualization of cellular processes, developmental biology studies, and investigations into life mechanics at the microscopic level.
published_date 2024-09-03T05:25:11Z
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