No Cover Image

Journal article 362 views 73 downloads

How to obtain physiologically relevant cardiovascular data with students using chick embryo ventricular cardiomyocytes

Nicholas S. Freestone Orcid Logo, Laura F. Corns Orcid Logo, James L. Rouse Orcid Logo, Aidan Seeley Orcid Logo

Advances in Physiology Education, Volume: 49, Issue: 4, Pages: 1026 - 1033

Swansea University Author: Aidan Seeley Orcid Logo

  • 70333.VoR.pdf

    PDF | Version of Record

    © 2025 The Authors. Licensed under a Creative Commons Attribution CC-BY-NC 4.0 license.

    Download (1.44MB)

Check full text

DOI (Published version): 10.1152/advan.00215.2024

Abstract

The chick embryo ventricular cardiomyocyte model provides students easy access to experiments involving fundamental features of cardiac cell physiology and pharmacology. With standard physiology teaching laboratories and basic cell culture equipment, spontaneously beating colonies of electrically co...

Full description

Published in: Advances in Physiology Education
ISSN: 1043-4046 1522-1229
Published: American Physiological Society 2025
Online Access: Check full text

URI: https://cronfa.swan.ac.uk/Record/cronfa70333
first_indexed 2025-09-11T11:25:02Z
last_indexed 2025-10-24T07:55:52Z
id cronfa70333
recordtype SURis
fullrecord <?xml version="1.0"?><rfc1807><datestamp>2025-10-22T15:17:46.3747597</datestamp><bib-version>v2</bib-version><id>70333</id><entry>2025-09-11</entry><title>How to obtain physiologically relevant cardiovascular data with students using chick embryo ventricular cardiomyocytes</title><swanseaauthors><author><sid>c69dba86b3ccf9a140b67b7e97d68bba</sid><ORCID>0000-0001-7085-4296</ORCID><firstname>Aidan</firstname><surname>Seeley</surname><name>Aidan Seeley</name><active>true</active><ethesisStudent>false</ethesisStudent></author></swanseaauthors><date>2025-09-11</date><deptcode>MEDS</deptcode><abstract>The chick embryo ventricular cardiomyocyte model provides students easy access to experiments involving fundamental features of cardiac cell physiology and pharmacology. With standard physiology teaching laboratories and basic cell culture equipment, spontaneously beating colonies of electrically connected cardiomyocytes can be obtained by the students themselves. Students learn aseptic techniques and cell culture alongside experiments illustrating, at the simplest level of experimentation, how beating rate can be altered physiologically or pharmacologically. In the typical course of the type of experiments presented here, students first observe the effect of temperature (beating rates decline to a third going from 37&#xB0;C to room temperature; e.g., to 40 from 130 beats/min) and media change (beating rates increase up to 50%) before moving on to the pharmacological characterization of various receptors in these cells. Most obviously, in the cardiac cell context, this involves drugs acting on &#x3B2;-adrenoceptor subtypes. Students can obtain predictable dose-dependent increases in beating rates (up to maximal 100% increases in beating rate; from &#x223C;100 to 200 beats/min typically) with the addition of stimulatory &#x3B2;-adrenoceptor agonists (e.g., isoproterenol) but also observe dose-dependent decreases in beating rate with &#x3B2;3-adrenoceptor agonists (reducing beating rate by up to a third). Consequently, &#x201C;classical&#x201D; log dose-response curves can be obtained in the &#x201C;real world,&#x201D; enhancing student understanding of fundamental mechanisms of drug action. Although these experiments focus on physiological and pharmacological techniques, the model can be extended to encompass biochemical or molecular biological studies in terms of intracellular signaling systems activated and protein expression patterns.</abstract><type>Journal Article</type><journal>Advances in Physiology Education</journal><volume>49</volume><journalNumber>4</journalNumber><paginationStart>1026</paginationStart><paginationEnd>1033</paginationEnd><publisher>American Physiological Society</publisher><placeOfPublication/><isbnPrint/><isbnElectronic/><issnPrint>1043-4046</issnPrint><issnElectronic>1522-1229</issnElectronic><keywords>chick embryo cardiomyocytes; humane experimental technique; partial replacement; 3Rs</keywords><publishedDay>1</publishedDay><publishedMonth>12</publishedMonth><publishedYear>2025</publishedYear><publishedDate>2025-12-01</publishedDate><doi>10.1152/advan.00215.2024</doi><url/><notes/><college>COLLEGE NANME</college><department>Medical School</department><CollegeCode>COLLEGE CODE</CollegeCode><DepartmentCode>MEDS</DepartmentCode><institution>Swansea University</institution><apcterm>Another institution paid the OA fee</apcterm><funders/><projectreference/><lastEdited>2025-10-22T15:17:46.3747597</lastEdited><Created>2025-09-11T12:23:45.6563080</Created><path><level id="1">Faculty of Medicine, Health and Life Sciences</level><level id="2">Swansea University Medical School - Biomedical Science</level></path><authors><author><firstname>Nicholas S.</firstname><surname>Freestone</surname><orcid>0009-0002-8626-7904</orcid><order>1</order></author><author><firstname>Laura F.</firstname><surname>Corns</surname><orcid>0000-0003-1139-3527</orcid><order>2</order></author><author><firstname>James L.</firstname><surname>Rouse</surname><orcid>0000-0001-8457-4623</orcid><order>3</order></author><author><firstname>Aidan</firstname><surname>Seeley</surname><orcid>0000-0001-7085-4296</orcid><order>4</order></author></authors><documents><document><filename>70333__35445__d536269e1b074e3b9c951faf6221023e.pdf</filename><originalFilename>70333.VoR.pdf</originalFilename><uploaded>2025-10-22T15:13:31.0144869</uploaded><type>Output</type><contentLength>1510375</contentLength><contentType>application/pdf</contentType><version>Version of Record</version><cronfaStatus>true</cronfaStatus><documentNotes>&#xA9; 2025 The Authors. Licensed under a Creative Commons Attribution CC-BY-NC 4.0 license.</documentNotes><copyrightCorrect>true</copyrightCorrect><language>eng</language><licence>https://creativecommons.org/licenses/by-nc/4.0/</licence></document></documents><OutputDurs/></rfc1807>
spelling 2025-10-22T15:17:46.3747597 v2 70333 2025-09-11 How to obtain physiologically relevant cardiovascular data with students using chick embryo ventricular cardiomyocytes c69dba86b3ccf9a140b67b7e97d68bba 0000-0001-7085-4296 Aidan Seeley Aidan Seeley true false 2025-09-11 MEDS The chick embryo ventricular cardiomyocyte model provides students easy access to experiments involving fundamental features of cardiac cell physiology and pharmacology. With standard physiology teaching laboratories and basic cell culture equipment, spontaneously beating colonies of electrically connected cardiomyocytes can be obtained by the students themselves. Students learn aseptic techniques and cell culture alongside experiments illustrating, at the simplest level of experimentation, how beating rate can be altered physiologically or pharmacologically. In the typical course of the type of experiments presented here, students first observe the effect of temperature (beating rates decline to a third going from 37°C to room temperature; e.g., to 40 from 130 beats/min) and media change (beating rates increase up to 50%) before moving on to the pharmacological characterization of various receptors in these cells. Most obviously, in the cardiac cell context, this involves drugs acting on β-adrenoceptor subtypes. Students can obtain predictable dose-dependent increases in beating rates (up to maximal 100% increases in beating rate; from ∼100 to 200 beats/min typically) with the addition of stimulatory β-adrenoceptor agonists (e.g., isoproterenol) but also observe dose-dependent decreases in beating rate with β3-adrenoceptor agonists (reducing beating rate by up to a third). Consequently, “classical” log dose-response curves can be obtained in the “real world,” enhancing student understanding of fundamental mechanisms of drug action. Although these experiments focus on physiological and pharmacological techniques, the model can be extended to encompass biochemical or molecular biological studies in terms of intracellular signaling systems activated and protein expression patterns. Journal Article Advances in Physiology Education 49 4 1026 1033 American Physiological Society 1043-4046 1522-1229 chick embryo cardiomyocytes; humane experimental technique; partial replacement; 3Rs 1 12 2025 2025-12-01 10.1152/advan.00215.2024 COLLEGE NANME Medical School COLLEGE CODE MEDS Swansea University Another institution paid the OA fee 2025-10-22T15:17:46.3747597 2025-09-11T12:23:45.6563080 Faculty of Medicine, Health and Life Sciences Swansea University Medical School - Biomedical Science Nicholas S. Freestone 0009-0002-8626-7904 1 Laura F. Corns 0000-0003-1139-3527 2 James L. Rouse 0000-0001-8457-4623 3 Aidan Seeley 0000-0001-7085-4296 4 70333__35445__d536269e1b074e3b9c951faf6221023e.pdf 70333.VoR.pdf 2025-10-22T15:13:31.0144869 Output 1510375 application/pdf Version of Record true © 2025 The Authors. Licensed under a Creative Commons Attribution CC-BY-NC 4.0 license. true eng https://creativecommons.org/licenses/by-nc/4.0/
title How to obtain physiologically relevant cardiovascular data with students using chick embryo ventricular cardiomyocytes
spellingShingle How to obtain physiologically relevant cardiovascular data with students using chick embryo ventricular cardiomyocytes
Aidan Seeley
title_short How to obtain physiologically relevant cardiovascular data with students using chick embryo ventricular cardiomyocytes
title_full How to obtain physiologically relevant cardiovascular data with students using chick embryo ventricular cardiomyocytes
title_fullStr How to obtain physiologically relevant cardiovascular data with students using chick embryo ventricular cardiomyocytes
title_full_unstemmed How to obtain physiologically relevant cardiovascular data with students using chick embryo ventricular cardiomyocytes
title_sort How to obtain physiologically relevant cardiovascular data with students using chick embryo ventricular cardiomyocytes
author_id_str_mv c69dba86b3ccf9a140b67b7e97d68bba
author_id_fullname_str_mv c69dba86b3ccf9a140b67b7e97d68bba_***_Aidan Seeley
author Aidan Seeley
author2 Nicholas S. Freestone
Laura F. Corns
James L. Rouse
Aidan Seeley
format Journal article
container_title Advances in Physiology Education
container_volume 49
container_issue 4
container_start_page 1026
publishDate 2025
institution Swansea University
issn 1043-4046
1522-1229
doi_str_mv 10.1152/advan.00215.2024
publisher American Physiological Society
college_str Faculty of Medicine, Health and Life Sciences
hierarchytype
hierarchy_top_id facultyofmedicinehealthandlifesciences
hierarchy_top_title Faculty of Medicine, Health and Life Sciences
hierarchy_parent_id facultyofmedicinehealthandlifesciences
hierarchy_parent_title Faculty of Medicine, Health and Life Sciences
department_str Swansea University Medical School - Biomedical Science{{{_:::_}}}Faculty of Medicine, Health and Life Sciences{{{_:::_}}}Swansea University Medical School - Biomedical Science
document_store_str 1
active_str 0
description The chick embryo ventricular cardiomyocyte model provides students easy access to experiments involving fundamental features of cardiac cell physiology and pharmacology. With standard physiology teaching laboratories and basic cell culture equipment, spontaneously beating colonies of electrically connected cardiomyocytes can be obtained by the students themselves. Students learn aseptic techniques and cell culture alongside experiments illustrating, at the simplest level of experimentation, how beating rate can be altered physiologically or pharmacologically. In the typical course of the type of experiments presented here, students first observe the effect of temperature (beating rates decline to a third going from 37°C to room temperature; e.g., to 40 from 130 beats/min) and media change (beating rates increase up to 50%) before moving on to the pharmacological characterization of various receptors in these cells. Most obviously, in the cardiac cell context, this involves drugs acting on β-adrenoceptor subtypes. Students can obtain predictable dose-dependent increases in beating rates (up to maximal 100% increases in beating rate; from ∼100 to 200 beats/min typically) with the addition of stimulatory β-adrenoceptor agonists (e.g., isoproterenol) but also observe dose-dependent decreases in beating rate with β3-adrenoceptor agonists (reducing beating rate by up to a third). Consequently, “classical” log dose-response curves can be obtained in the “real world,” enhancing student understanding of fundamental mechanisms of drug action. Although these experiments focus on physiological and pharmacological techniques, the model can be extended to encompass biochemical or molecular biological studies in terms of intracellular signaling systems activated and protein expression patterns.
published_date 2025-12-01T05:31:11Z
_version_ 1856805662805196800
score 11.09611

Similar Items