Heterogeneous and Flexible Transmission of mcr-1 in Hospital-Associated Escherichia coli

Shen, Yingbo; Wu, Zuowei; Wang, Yang; Zhang, Rong; Zhou, Hong-Wei; Wang, Shaolin; Lei, Lei; Li, Mei; Cai, Jiachang; Tyrrell, Jon; Tian, Guo-Bao; Wu, Congming; Zhang, Qijing; Shen, Jianzhong; Walsh, Timothy R.; Shen, Zhangqi

doi:10.1128/mbio.00943-18

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Heterogeneous and Flexible Transmission of mcr-1 in Hospital-Associated Escherichia coli

Yingbo Shen, Zuowei Wu, Yang Wang, Rong Zhang, Hong-Wei Zhou, Shaolin Wang

, Lei Lei, Mei Li, Jiachang Cai, Jon Tyrrell

, Guo-Bao Tian, Congming Wu, Qijing Zhang, Jianzhong Shen, Timothy R. Walsh, Zhangqi Shen

mBio, Volume: 9, Issue: 4

Swansea University Author: Jon Tyrrell

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DOI (Published version): 10.1128/mbio.00943-18

Abstract

The recent emergence of a transferable colistin resistance mechanism, MCR-1, has gained global attention because of its threat to clinical treatment of infections caused by multidrug-resistant Gram-negative bacteria. However, the possible transmission route of mcr-1 among Enterobacteriaceae species...

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Published in:	mBio
ISSN:	2161-2129 2150-7511
Published:	American Society for Microbiology 2018
Online Access:	Check full text
URI:	https://cronfa.swan.ac.uk/Record/cronfa70426

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Here, we present a comprehensive genomic analysis of Escherichia coli isolates collected in a hospital in Hangzhou, China. We found that mcr-1-carrying isolates from clinical infections and feces of inpatients and healthy volunteers were genetically diverse and were not closely related phylogenetically, suggesting that clonal expansion is not involved in the spread of mcr-1. The mcr-1 gene was found on either chromosomes or plasmids, but in most of the E. coli isolates, mcr-1 was carried on plasmids. The genetic context of the plasmids showed considerable diversity as evidenced by the different functional insertion sequence (IS) elements, toxin-antitoxin (TA) systems, heavy metal resistance determinants, and Rep proteins of broad-host-range plasmids. Additionally, the genomic analysis revealed nosocomial transmission of mcr-1 and the coexistence of mcr-1 with other genes encoding β-lactamases and fluoroquinolone resistance in the E. coli isolates. 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spelling	2025-10-16T15:29:00.6416389 v2 70426 2025-09-21 Heterogeneous and Flexible Transmission of mcr-1 in Hospital-Associated Escherichia coli ad510c73555adf718387af219e235a6e 0000-0001-8565-2590 Jon Tyrrell Jon Tyrrell true false 2025-09-21 MEDS The recent emergence of a transferable colistin resistance mechanism, MCR-1, has gained global attention because of its threat to clinical treatment of infections caused by multidrug-resistant Gram-negative bacteria. However, the possible transmission route of mcr-1 among Enterobacteriaceae species in clinical settings is largely unknown. Here, we present a comprehensive genomic analysis of Escherichia coli isolates collected in a hospital in Hangzhou, China. We found that mcr-1-carrying isolates from clinical infections and feces of inpatients and healthy volunteers were genetically diverse and were not closely related phylogenetically, suggesting that clonal expansion is not involved in the spread of mcr-1. The mcr-1 gene was found on either chromosomes or plasmids, but in most of the E. coli isolates, mcr-1 was carried on plasmids. The genetic context of the plasmids showed considerable diversity as evidenced by the different functional insertion sequence (IS) elements, toxin-antitoxin (TA) systems, heavy metal resistance determinants, and Rep proteins of broad-host-range plasmids. Additionally, the genomic analysis revealed nosocomial transmission of mcr-1 and the coexistence of mcr-1 with other genes encoding β-lactamases and fluoroquinolone resistance in the E. coli isolates. These findings indicate that mcr-1 is heterogeneously disseminated in both commensal and pathogenic strains of E. coli, suggest the high flexibility of this gene in its association with diverse genetic backgrounds of the hosts, and provide new insights into the genome epidemiology of mcr-1 among hospital-associated E. coli strains. Journal Article mBio 9 4 American Society for Microbiology 2161-2129 2150-7511 E. coli, genetic diversity, mcr-1, population genomics, transmission 5 9 2018 2018-09-05 10.1128/mbio.00943-18 COLLEGE NANME Medical School COLLEGE CODE MEDS Swansea University Another institution paid the OA fee This work was supported in part by the National Natural Science Foundation of China (grants 31672604 and 31422055) and the Natural Science Foundation of Zhejiang Province (2013C33G2010505), Higher Education Funding Council and European Commission, United Kingdom. 2025-10-16T15:29:00.6416389 2025-09-21T18:13:15.2418348 Faculty of Medicine, Health and Life Sciences Swansea University Medical School - Biomedical Science Yingbo Shen 1 Zuowei Wu 2 Yang Wang 3 Rong Zhang 4 Hong-Wei Zhou 5 Shaolin Wang 0000-0003-0866-4584 6 Lei Lei 7 Mei Li 8 Jiachang Cai 9 Jon Tyrrell 0000-0001-8565-2590 10 Guo-Bao Tian 11 Congming Wu 12 Qijing Zhang 13 Jianzhong Shen 14 Timothy R. Walsh 15 Zhangqi Shen 16 70426__35371__18d76d42ae9043a5917afff812610e5b.pdf 70426.VoR.pdf 2025-10-16T15:26:50.9469917 Output 3551167 application/pdf Version of Record true © 2018 Shen et al. This is an open-access article distributed under the terms of the Creative Commons Attribution 4.0 International license. true eng https://creativecommons.org/licenses/by/4.0/
title	Heterogeneous and Flexible Transmission of mcr-1 in Hospital-Associated Escherichia coli
spellingShingle	Heterogeneous and Flexible Transmission of mcr-1 in Hospital-Associated Escherichia coli Jon Tyrrell
title_short	Heterogeneous and Flexible Transmission of mcr-1 in Hospital-Associated Escherichia coli
title_full	Heterogeneous and Flexible Transmission of mcr-1 in Hospital-Associated Escherichia coli
title_fullStr	Heterogeneous and Flexible Transmission of mcr-1 in Hospital-Associated Escherichia coli
title_full_unstemmed	Heterogeneous and Flexible Transmission of mcr-1 in Hospital-Associated Escherichia coli
title_sort	Heterogeneous and Flexible Transmission of mcr-1 in Hospital-Associated Escherichia coli
author_id_str_mv	ad510c73555adf718387af219e235a6e
author_id_fullname_str_mv	ad510c73555adf718387af219e235a6e_***_Jon Tyrrell
author	Jon Tyrrell
author2	Yingbo Shen Zuowei Wu Yang Wang Rong Zhang Hong-Wei Zhou Shaolin Wang Lei Lei Mei Li Jiachang Cai Jon Tyrrell Guo-Bao Tian Congming Wu Qijing Zhang Jianzhong Shen Timothy R. Walsh Zhangqi Shen
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container_title	mBio
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publishDate	2018
institution	Swansea University
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doi_str_mv	10.1128/mbio.00943-18
publisher	American Society for Microbiology
college_str	Faculty of Medicine, Health and Life Sciences
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department_str	Swansea University Medical School - Biomedical Science{{{_:::_}}}Faculty of Medicine, Health and Life Sciences{{{_:::_}}}Swansea University Medical School - Biomedical Science
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description	The recent emergence of a transferable colistin resistance mechanism, MCR-1, has gained global attention because of its threat to clinical treatment of infections caused by multidrug-resistant Gram-negative bacteria. However, the possible transmission route of mcr-1 among Enterobacteriaceae species in clinical settings is largely unknown. Here, we present a comprehensive genomic analysis of Escherichia coli isolates collected in a hospital in Hangzhou, China. We found that mcr-1-carrying isolates from clinical infections and feces of inpatients and healthy volunteers were genetically diverse and were not closely related phylogenetically, suggesting that clonal expansion is not involved in the spread of mcr-1. The mcr-1 gene was found on either chromosomes or plasmids, but in most of the E. coli isolates, mcr-1 was carried on plasmids. The genetic context of the plasmids showed considerable diversity as evidenced by the different functional insertion sequence (IS) elements, toxin-antitoxin (TA) systems, heavy metal resistance determinants, and Rep proteins of broad-host-range plasmids. Additionally, the genomic analysis revealed nosocomial transmission of mcr-1 and the coexistence of mcr-1 with other genes encoding β-lactamases and fluoroquinolone resistance in the E. coli isolates. These findings indicate that mcr-1 is heterogeneously disseminated in both commensal and pathogenic strains of E. coli, suggest the high flexibility of this gene in its association with diverse genetic backgrounds of the hosts, and provide new insights into the genome epidemiology of mcr-1 among hospital-associated E. coli strains.
published_date	2018-09-05T05:26:06Z
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score	11.089572

Heterogeneous and Flexible Transmission of mcr-1 in Hospital-Associated Escherichia coli

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