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A Novel β/ε Subunit Combination Expands the Tri-Subunit Acyl-CoA Carboxylase Repertoire in Streptomyces coelicolor
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Xue Yu ,
Yujie Wu ,
Xiaomin Niu,
Ximing Chen,
Tuo Chen,
Wei Zhang,
Guangxiu Liu,
Paul Dyson
Microorganisms, Volume: 14, Issue: 4, Start page: 733
Swansea University Author:
Paul Dyson
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DOI (Published version): 10.3390/microorganisms14040733
Abstract
Acyl-CoA carboxylase (YCC) complexes generate essential starter and extender units for fatty acid and polyketide biosynthesis in Actinobacteria. In Streptomyces coelicolor, two tri-subunit YCC complexes, acetyl-CoA carboxylase (ACC) and propionyl-CoA carboxylase (PCC), have been characterized. Howev...
| Published in: | Microorganisms |
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| ISSN: | 2076-2607 |
| Published: |
MDPI AG
2026
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| Online Access: |
Check full text
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| URI: | https://cronfa.swan.ac.uk/Record/cronfa71717 |
| Abstract: |
Acyl-CoA carboxylase (YCC) complexes generate essential starter and extender units for fatty acid and polyketide biosynthesis in Actinobacteria. In Streptomyces coelicolor, two tri-subunit YCC complexes, acetyl-CoA carboxylase (ACC) and propionyl-CoA carboxylase (PCC), have been characterized. However, comparative genomic analyses indicate that β/ε subunits are more diversified than currently appreciated. Here, we identify a previously unrecognized β/ε pair, AccB2 and AccE2, and demonstrate that they assemble with the canonical α subunit to form a functional YCC complex. Both genes are transcribed in vivo, and co-immunoprecipitation (Co-IP) reveals association with AccA1 and AccA2, with AccE2 showing stronger relative association with AccA1-containing pull-downs. In vitro reconstitution confirms carboxylation activity toward acetyl-CoA, propionyl-CoA, and butyryl-CoA, which is strongly dependent on AccE2. These findings expand the YCC repertoire in S. coelicolor and support a modular assembly model in which alternative β/ε combinations contribute to functional diversification of YCC complexes. |
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| Keywords: |
acyl-CoA carboxylase; Streptomyces coelicolor; tri-subunit complex; β/ε subunits |
| College: |
Faculty of Medicine, Health and Life Sciences |
| Funders: |
This work was financially supported by the Scientific Project of Gansu Province (22ZD6WA035) and the National Natural Science Foundation of China (U22A20451). |
| Issue: |
4 |
| Start Page: |
733 |