Journal article 1117 views
Cyclization of a Cellular Dipentaenone by Streptomyces coelicolor Cytochrome P450 154A1 without Oxidation/Reduction
,
L Lei,
FP Guenguerich,
Diane Kelly
Journal of the American Chemical Society, Volume: 132, Issue: 43, Pages: 15173 - 15175
Swansea University Authors:
Steven Kelly , Diane Kelly
Full text not available from this repository: check for access using links below.
DOI (Published version): 10.1021/ja107801v
Abstract
We report a comprehensive genetic, metabolomic, and biochemical study on the catalytic properties of Streptomyces coelicolor cytochrome P450 (P450) 154A1, known to have a unique heme orientation in its crystal structure. Deletion of the P450 154A1 gene compromised the long-term stability of the bact...
| Published in: | Journal of the American Chemical Society |
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| ISSN: | 0002-7863 1520-5126 |
| Published: |
1155 16TH ST, NW, WASHINGTON, DC 20036 USA
AMER CHEMICAL SOC
2010
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| Online Access: |
Check full text
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| URI: | https://cronfa.swan.ac.uk/Record/cronfa9901 |
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| Abstract: |
We report a comprehensive genetic, metabolomic, and biochemical study on the catalytic properties of Streptomyces coelicolor cytochrome P450 (P450) 154A1, known to have a unique heme orientation in its crystal structure. Deletion of the P450 154A1 gene compromised the long-term stability of the bacterial spores. A novel dipentaenone (1) with a high degree of conjugation was identified as an endogenous substrate of P450 154A1 using a metabolomics approach. The biotransformation of 1 by P450 154A1 was shown to be an unexpected intramolecular cyclization to a Paterno-Buchi-like product, without oxidation/reduction |
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| Keywords: |
DRUG DISCOVERY; A3(2); IDENTIFICATION; MODEL |
| College: |
Faculty of Medicine, Health and Life Sciences |
| Issue: |
43 |
| Start Page: |
15173 |
| End Page: |
15175 |