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Triclosan anatagonizes fluconazole activity against candida albicans / J Higgins; E Pinjon; HN Oltean; TC White; SL Kelly; CM Martel; DJ Sulivan; DC Coleman; GP Moran; Steven Kelly; Claire Hull

Journal of Dental Research, Volume: 91, Issue: 1, Pages: 65 - 70

Swansea University Authors: Steven, Kelly, Claire, Hull

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DOI (Published version): 10.1177/0022034511425046

Abstract

Triclosan is a broad-spectrum antimicrobial compound commonly used in oral hygiene products. Investigation of its activity against Candida albicans showed that triclosan was fungicidal at concentrations of 16 mg/L. However, at subinhibitory concentrations (0.5-2 mg/L), triclosan antagonized the acti...

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Published in: Journal of Dental Research
Published: 2012
URI: https://cronfa.swan.ac.uk/Record/cronfa10166
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Abstract: Triclosan is a broad-spectrum antimicrobial compound commonly used in oral hygiene products. Investigation of its activity against Candida albicans showed that triclosan was fungicidal at concentrations of 16 mg/L. However, at subinhibitory concentrations (0.5-2 mg/L), triclosan antagonized the activity of fluconazole. Although triclosan induced CDR1 expression in C. albicans, antagonism was still observed in cdr1 Delta and cdr2 Delta strains. Triclosan did not affect fluconazole uptake or alter total membrane sterol content, but did induce the expression of FAS1 and FAS2, indicating that its mode of action may involve inhibition of fatty acid synthesis, as it does in prokaryotes. However, FAS2 mutants did not exhibit increased susceptibility to triclosan, and overexpression of both FAS1 and FAS2 alleles did not alter triclosan susceptibility. Unexpectedly, the antagonistic effect was specific for C. albicans under hypha-inducing conditions and was absent in the non-filamentous efg1 Delta strain. This antagonism may be due to the membranotropic activity of triclosan and the unique composition of hyphal membranes.
Keywords: triclosan; fluconazole; antagonism; Candida albicans; EFG1; hyphae
College: Swansea University Medical School
Issue: 1
Start Page: 65
End Page: 70