Journal article 596 views
Molecular characterization of a subgroup IE intron with wide distribution in the large subunit rRNA genes of dermatophyte fungi
Medical Mycology, Volume: 47, Issue: 6, Pages: 609 - 617
Swansea University Author:
Steven Kelly
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DOI (Published version): 10.1080/13693780802385445
Abstract
Group I introns have the ability to catalyse their own excision (self-splice) from pre-RNA, and are found in a wide range of eukaryotic organisms. In fungal nuclear genomes, they have been identified in the small subunit (SSU) and large subunit (LSU) of the ribosomal RNA gene. Sequencing of the 3...
Published in: | Medical Mycology |
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ISSN: | 1369-3786 1460-2709 |
Published: |
PARK SQUARE, MILTON PARK, ABINGDON OX14 4RN, OXON, ENGLAND
Oxford University Press (OUP)
2009
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URI: | https://cronfa.swan.ac.uk/Record/cronfa10333 |
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2021-10-29T09:37:00.9919464 v2 10333 2012-03-21 Molecular characterization of a subgroup IE intron with wide distribution in the large subunit rRNA genes of dermatophyte fungi b17cebaf09b4d737b9378a3581e3de93 0000-0001-7991-5040 Steven Kelly Steven Kelly true false 2012-03-21 BMS Group I introns have the ability to catalyse their own excision (self-splice) from pre-RNA, and are found in a wide range of eukaryotic organisms. In fungal nuclear genomes, they have been identified in the small subunit (SSU) and large subunit (LSU) of the ribosomal RNA gene. Sequencing of the 3' region of the LSU rRNA gene of the dermatophyte Trichophyton interdigitale revealed a 393 bp group I intron, Tin. 2563, containing the four characteristic conserved motifs (P, Q, R and S) essential for self-splicing. The predicted secondary structure revealed nine sets of conserved paired regions (P1-P9), with most similarity to a subgroup IE intron of the entomopathogenic hyphomycete Beauveria bassiana. Tin. 2563 was inserted at a site in the LSU rDNA corresponding to position 2563 of the Escherichia coli 23S rRNA. PCR and sequence analysis showed an intron to be present at an identical location in the LSU rDNA of many dermatophytes, although its distribution was erratic. In contrast, an intron was present at the same location in multiple isolates (n = 20) of the clinically important anthrophilic species Trichophyton rubrum and T. interdigitale. Conservation of intron insertion site, subgroup and P helix sequences showed intron genotyping to be unsuitable for strain identification in dermatophytes. Phylogenetic analysis of intron sequences from different dermatophyte species indicated that lateral transfer of the element was likely to be a rare event. Journal Article Medical Mycology 47 6 609 617 Oxford University Press (OUP) PARK SQUARE, MILTON PARK, ABINGDON OX14 4RN, OXON, ENGLAND 1369-3786 1460-2709 1 1 2009 2009-01-01 10.1080/13693780802385445 COLLEGE NANME Biomedical Sciences COLLEGE CODE BMS Swansea University 2021-10-29T09:37:00.9919464 2012-03-21T16:17:29.0000000 Faculty of Medicine, Health and Life Sciences Swansea University Medical School - Medicine Colin J. Jackson 1 Richard C. Barton 2 C. Graham Clark 3 Steven Kelly 0000-0001-7991-5040 4 |
title |
Molecular characterization of a subgroup IE intron with wide distribution in the large subunit rRNA genes of dermatophyte fungi |
spellingShingle |
Molecular characterization of a subgroup IE intron with wide distribution in the large subunit rRNA genes of dermatophyte fungi Steven Kelly |
title_short |
Molecular characterization of a subgroup IE intron with wide distribution in the large subunit rRNA genes of dermatophyte fungi |
title_full |
Molecular characterization of a subgroup IE intron with wide distribution in the large subunit rRNA genes of dermatophyte fungi |
title_fullStr |
Molecular characterization of a subgroup IE intron with wide distribution in the large subunit rRNA genes of dermatophyte fungi |
title_full_unstemmed |
Molecular characterization of a subgroup IE intron with wide distribution in the large subunit rRNA genes of dermatophyte fungi |
title_sort |
Molecular characterization of a subgroup IE intron with wide distribution in the large subunit rRNA genes of dermatophyte fungi |
author_id_str_mv |
b17cebaf09b4d737b9378a3581e3de93 |
author_id_fullname_str_mv |
b17cebaf09b4d737b9378a3581e3de93_***_Steven Kelly |
author |
Steven Kelly |
author2 |
Colin J. Jackson Richard C. Barton C. Graham Clark Steven Kelly |
format |
Journal article |
container_title |
Medical Mycology |
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47 |
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6 |
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609 |
publishDate |
2009 |
institution |
Swansea University |
issn |
1369-3786 1460-2709 |
doi_str_mv |
10.1080/13693780802385445 |
publisher |
Oxford University Press (OUP) |
college_str |
Faculty of Medicine, Health and Life Sciences |
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facultyofmedicinehealthandlifesciences |
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Faculty of Medicine, Health and Life Sciences |
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facultyofmedicinehealthandlifesciences |
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Faculty of Medicine, Health and Life Sciences |
department_str |
Swansea University Medical School - Medicine{{{_:::_}}}Faculty of Medicine, Health and Life Sciences{{{_:::_}}}Swansea University Medical School - Medicine |
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description |
Group I introns have the ability to catalyse their own excision (self-splice) from pre-RNA, and are found in a wide range of eukaryotic organisms. In fungal nuclear genomes, they have been identified in the small subunit (SSU) and large subunit (LSU) of the ribosomal RNA gene. Sequencing of the 3' region of the LSU rRNA gene of the dermatophyte Trichophyton interdigitale revealed a 393 bp group I intron, Tin. 2563, containing the four characteristic conserved motifs (P, Q, R and S) essential for self-splicing. The predicted secondary structure revealed nine sets of conserved paired regions (P1-P9), with most similarity to a subgroup IE intron of the entomopathogenic hyphomycete Beauveria bassiana. Tin. 2563 was inserted at a site in the LSU rDNA corresponding to position 2563 of the Escherichia coli 23S rRNA. PCR and sequence analysis showed an intron to be present at an identical location in the LSU rDNA of many dermatophytes, although its distribution was erratic. In contrast, an intron was present at the same location in multiple isolates (n = 20) of the clinically important anthrophilic species Trichophyton rubrum and T. interdigitale. Conservation of intron insertion site, subgroup and P helix sequences showed intron genotyping to be unsuitable for strain identification in dermatophytes. Phylogenetic analysis of intron sequences from different dermatophyte species indicated that lateral transfer of the element was likely to be a rare event. |
published_date |
2009-01-01T03:11:41Z |
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1763750027239358464 |
score |
10.950252 |