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Identification of Clinical Isolates of Alpha-Hemolytic Streptococci by 16S rRNA Gene Sequencing, Matrix-Assisted Laser Desorption Ionization-Time of Flight Mass Spectrometry Using MALDI Biotyper, and Conventional Phenotypic Method...

A. P Davies, M Reid, S. J Hadfield, S Johnston, J Mikhail, L. G Harris, H. F Jenkinson, N Berry, A. M Lewis, K El-Bouri, D Mack, Jane Mikhail, Angharad Davies Orcid Logo, Llinos Harris Orcid Logo

Journal of Clinical Microbiology, Volume: 50, Issue: 12, Start page: 4087

Swansea University Authors: Jane Mikhail, Angharad Davies Orcid Logo, Llinos Harris Orcid Logo

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DOI (Published version): 10.1128/JCM.02387-12

Abstract

Fifty-six α-haemolytic streptococcal isolates were identified using MALDI Biotyper MS (Bruker Daltonics), API 20 Strep (bioMerieux) and BD Phoenix (Becton Dickinson). Gold-standard identification was 16S rRNA gene sequence analysis with 16S-23S rRNA intergenic spacer sequencing. MALDI Biotyper corre...

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Published in: Journal of Clinical Microbiology
ISSN: 0095-1137
Published: 2012
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URI: https://cronfa.swan.ac.uk/Record/cronfa12782
Abstract: Fifty-six α-haemolytic streptococcal isolates were identified using MALDI Biotyper MS (Bruker Daltonics), API 20 Strep (bioMerieux) and BD Phoenix (Becton Dickinson). Gold-standard identification was 16S rRNA gene sequence analysis with 16S-23S rRNA intergenic spacer sequencing. MALDI Biotyper correctly speciated 43% of isolates,, BD Phoenix 35% and API 20 Strep 26%.
College: Faculty of Medicine, Health and Life Sciences
Issue: 12
Start Page: 4087

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