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Identification of Clinical Isolates of Alpha-Hemolytic Streptococci by 16S rRNA Gene Sequencing, Matrix-Assisted Laser Desorption Ionization-Time of Flight Mass Spectrometry Using MALDI Biotyper, and Conventional Phenotypic Method... / A. P Davies; M Reid; S. J Hadfield; S Johnston; J Mikhail; L. G Harris; H. F Jenkinson; N Berry; A. M Lewis; K El-Bouri; D Mack; Jane Mikhail; Angharad Davies; Llinos Harris

Journal of Clinical Microbiology, Volume: 50, Issue: 12, Start page: 4087

Swansea University Authors: Jane, Mikhail, Angharad, Davies, Llinos, Harris

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DOI (Published version): 10.1128/JCM.02387-12

Abstract

Fifty-six α-haemolytic streptococcal isolates were identified using MALDI Biotyper MS (Bruker Daltonics), API 20 Strep (bioMerieux) and BD Phoenix (Becton Dickinson). Gold-standard identification was 16S rRNA gene sequence analysis with 16S-23S rRNA intergenic spacer sequencing. MALDI Biotyper corre...

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Published in: Journal of Clinical Microbiology
ISSN: 0095-1137
Published: 2012
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URI: https://cronfa.swan.ac.uk/Record/cronfa12782
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last_indexed 2018-02-09T04:43:08Z
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spelling 2013-08-08T10:40:31.7602799 v2 12782 2012-09-22 Identification of Clinical Isolates of Alpha-Hemolytic Streptococci by 16S rRNA Gene Sequencing, Matrix-Assisted Laser Desorption Ionization-Time of Flight Mass Spectrometry Using MALDI Biotyper, and Conventional Phenotypic Methods: a Comparison f3806e5ff186dc9a67518a780ebff675 Jane Mikhail Jane Mikhail true false 62586459693e05b2e1063967e76883f1 0000-0003-4190-8894 Angharad Davies Angharad Davies true false dc70f9d4badbbdb5d467fd321986d173 0000-0002-0295-3038 Llinos Harris Llinos Harris true false 2012-09-22 Fifty-six α-haemolytic streptococcal isolates were identified using MALDI Biotyper MS (Bruker Daltonics), API 20 Strep (bioMerieux) and BD Phoenix (Becton Dickinson). Gold-standard identification was 16S rRNA gene sequence analysis with 16S-23S rRNA intergenic spacer sequencing. MALDI Biotyper correctly speciated 43% of isolates,, BD Phoenix 35% and API 20 Strep 26%. Journal Article Journal of Clinical Microbiology 50 12 4087 0095-1137 31 12 2012 2012-12-31 10.1128/JCM.02387-12 COLLEGE NANME COLLEGE CODE Swansea University 2013-08-08T10:40:31.7602799 2012-09-22T11:03:39.1547593 Swansea University Medical School Medicine A. P Davies 1 M Reid 2 S. J Hadfield 3 S Johnston 4 J Mikhail 5 L. G Harris 6 H. F Jenkinson 7 N Berry 8 A. M Lewis 9 K El-Bouri 10 D Mack 11 Jane Mikhail 12 Angharad Davies 0000-0003-4190-8894 13 Llinos Harris 0000-0002-0295-3038 14
title Identification of Clinical Isolates of Alpha-Hemolytic Streptococci by 16S rRNA Gene Sequencing, Matrix-Assisted Laser Desorption Ionization-Time of Flight Mass Spectrometry Using MALDI Biotyper, and Conventional Phenotypic Methods: a Comparison
spellingShingle Identification of Clinical Isolates of Alpha-Hemolytic Streptococci by 16S rRNA Gene Sequencing, Matrix-Assisted Laser Desorption Ionization-Time of Flight Mass Spectrometry Using MALDI Biotyper, and Conventional Phenotypic Methods: a Comparison
Jane, Mikhail
Angharad, Davies
Llinos, Harris
title_short Identification of Clinical Isolates of Alpha-Hemolytic Streptococci by 16S rRNA Gene Sequencing, Matrix-Assisted Laser Desorption Ionization-Time of Flight Mass Spectrometry Using MALDI Biotyper, and Conventional Phenotypic Methods: a Comparison
title_full Identification of Clinical Isolates of Alpha-Hemolytic Streptococci by 16S rRNA Gene Sequencing, Matrix-Assisted Laser Desorption Ionization-Time of Flight Mass Spectrometry Using MALDI Biotyper, and Conventional Phenotypic Methods: a Comparison
title_fullStr Identification of Clinical Isolates of Alpha-Hemolytic Streptococci by 16S rRNA Gene Sequencing, Matrix-Assisted Laser Desorption Ionization-Time of Flight Mass Spectrometry Using MALDI Biotyper, and Conventional Phenotypic Methods: a Comparison
title_full_unstemmed Identification of Clinical Isolates of Alpha-Hemolytic Streptococci by 16S rRNA Gene Sequencing, Matrix-Assisted Laser Desorption Ionization-Time of Flight Mass Spectrometry Using MALDI Biotyper, and Conventional Phenotypic Methods: a Comparison
title_sort Identification of Clinical Isolates of Alpha-Hemolytic Streptococci by 16S rRNA Gene Sequencing, Matrix-Assisted Laser Desorption Ionization-Time of Flight Mass Spectrometry Using MALDI Biotyper, and Conventional Phenotypic Methods: a Comparison
author_id_str_mv f3806e5ff186dc9a67518a780ebff675
62586459693e05b2e1063967e76883f1
dc70f9d4badbbdb5d467fd321986d173
author_id_fullname_str_mv f3806e5ff186dc9a67518a780ebff675_***_Jane, Mikhail
62586459693e05b2e1063967e76883f1_***_Angharad, Davies
dc70f9d4badbbdb5d467fd321986d173_***_Llinos, Harris
author Jane, Mikhail
Angharad, Davies
Llinos, Harris
author2 A. P Davies
M Reid
S. J Hadfield
S Johnston
J Mikhail
L. G Harris
H. F Jenkinson
N Berry
A. M Lewis
K El-Bouri
D Mack
Jane Mikhail
Angharad Davies
Llinos Harris
format Journal article
container_title Journal of Clinical Microbiology
container_volume 50
container_issue 12
container_start_page 4087
publishDate 2012
institution Swansea University
issn 0095-1137
doi_str_mv 10.1128/JCM.02387-12
college_str Swansea University Medical School
hierarchytype
hierarchy_top_id swanseauniversitymedicalschool
hierarchy_top_title Swansea University Medical School
hierarchy_parent_id swanseauniversitymedicalschool
hierarchy_parent_title Swansea University Medical School
department_str Medicine{{{_:::_}}}Swansea University Medical School{{{_:::_}}}Medicine
document_store_str 0
active_str 0
description Fifty-six α-haemolytic streptococcal isolates were identified using MALDI Biotyper MS (Bruker Daltonics), API 20 Strep (bioMerieux) and BD Phoenix (Becton Dickinson). Gold-standard identification was 16S rRNA gene sequence analysis with 16S-23S rRNA intergenic spacer sequencing. MALDI Biotyper correctly speciated 43% of isolates,, BD Phoenix 35% and API 20 Strep 26%.
published_date 2012-12-31T03:21:34Z
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