Identification of Clinical Isolates of Alpha-Hemolytic Streptococci by 16S rRNA Gene Sequencing, Matrix-Assisted Laser Desorption Ionization-Time of Flight Mass Spectrometry Using MALDI Biotyper, and Conventional Phenotypic Methods: a Comparison

Davies, A. P; Reid, M; Hadfield, S. J; Johnston, S; Mikhail, J; Harris, L. G; Jenkinson, H. F; Berry, N; Lewis, A. M; El-Bouri, K; Mack, D; Mikhail, Jane; Davies, Angharad; Harris, Llinos

doi:10.1128/JCM.02387-12

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Identification of Clinical Isolates of Alpha-Hemolytic Streptococci by 16S rRNA Gene Sequencing, Matrix-Assisted Laser Desorption Ionization-Time of Flight Mass Spectrometry Using MALDI Biotyper, and Conventional Phenotypic Method...

A. P Davies, M Reid, S. J Hadfield, S Johnston, J Mikhail, L. G Harris, H. F Jenkinson, N Berry, A. M Lewis, K El-Bouri, D Mack, Jane Mikhail, Angharad Davies

, Llinos Harris

Journal of Clinical Microbiology, Volume: 50, Issue: 12, Start page: 4087

Swansea University Authors: Jane Mikhail, Angharad Davies , Llinos Harris

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DOI (Published version): 10.1128/JCM.02387-12

Abstract

Fifty-six α-haemolytic streptococcal isolates were identified using MALDI Biotyper MS (Bruker Daltonics), API 20 Strep (bioMerieux) and BD Phoenix (Becton Dickinson). Gold-standard identification was 16S rRNA gene sequence analysis with 16S-23S rRNA intergenic spacer sequencing. MALDI Biotyper corre...

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Published in:	Journal of Clinical Microbiology
ISSN:	0095-1137
Published:	2012
Online Access:	Check full text
URI:	https://cronfa.swan.ac.uk/Record/cronfa12782
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first_indexed	2013-07-23T12:08:47Z
last_indexed	2018-02-09T04:43:08Z
id	cronfa12782
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spelling	2013-08-08T10:40:31.7602799 v2 12782 2012-09-22 Identification of Clinical Isolates of Alpha-Hemolytic Streptococci by 16S rRNA Gene Sequencing, Matrix-Assisted Laser Desorption Ionization-Time of Flight Mass Spectrometry Using MALDI Biotyper, and Conventional Phenotypic Methods: a Comparison f3806e5ff186dc9a67518a780ebff675 Jane Mikhail Jane Mikhail true false 62586459693e05b2e1063967e76883f1 0000-0003-4190-8894 Angharad Davies Angharad Davies true false dc70f9d4badbbdb5d467fd321986d173 0000-0002-0295-3038 Llinos Harris Llinos Harris true false 2012-09-22 FGMHL Fifty-six α-haemolytic streptococcal isolates were identified using MALDI Biotyper MS (Bruker Daltonics), API 20 Strep (bioMerieux) and BD Phoenix (Becton Dickinson). Gold-standard identification was 16S rRNA gene sequence analysis with 16S-23S rRNA intergenic spacer sequencing. MALDI Biotyper correctly speciated 43% of isolates,, BD Phoenix 35% and API 20 Strep 26%. Journal Article Journal of Clinical Microbiology 50 12 4087 0095-1137 31 12 2012 2012-12-31 10.1128/JCM.02387-12 COLLEGE NANME Medicine, Health and Life Science - Faculty COLLEGE CODE FGMHL Swansea University 2013-08-08T10:40:31.7602799 2012-09-22T11:03:39.1547593 Faculty of Medicine, Health and Life Sciences Swansea University Medical School - Medicine A. P Davies 1 M Reid 2 S. J Hadfield 3 S Johnston 4 J Mikhail 5 L. G Harris 6 H. F Jenkinson 7 N Berry 8 A. M Lewis 9 K El-Bouri 10 D Mack 11 Jane Mikhail 12 Angharad Davies 0000-0003-4190-8894 13 Llinos Harris 0000-0002-0295-3038 14
title	Identification of Clinical Isolates of Alpha-Hemolytic Streptococci by 16S rRNA Gene Sequencing, Matrix-Assisted Laser Desorption Ionization-Time of Flight Mass Spectrometry Using MALDI Biotyper, and Conventional Phenotypic Methods: a Comparison
spellingShingle	Identification of Clinical Isolates of Alpha-Hemolytic Streptococci by 16S rRNA Gene Sequencing, Matrix-Assisted Laser Desorption Ionization-Time of Flight Mass Spectrometry Using MALDI Biotyper, and Conventional Phenotypic Methods: a Comparison Jane Mikhail Angharad Davies Llinos Harris
title_short	Identification of Clinical Isolates of Alpha-Hemolytic Streptococci by 16S rRNA Gene Sequencing, Matrix-Assisted Laser Desorption Ionization-Time of Flight Mass Spectrometry Using MALDI Biotyper, and Conventional Phenotypic Methods: a Comparison
title_full	Identification of Clinical Isolates of Alpha-Hemolytic Streptococci by 16S rRNA Gene Sequencing, Matrix-Assisted Laser Desorption Ionization-Time of Flight Mass Spectrometry Using MALDI Biotyper, and Conventional Phenotypic Methods: a Comparison
title_fullStr	Identification of Clinical Isolates of Alpha-Hemolytic Streptococci by 16S rRNA Gene Sequencing, Matrix-Assisted Laser Desorption Ionization-Time of Flight Mass Spectrometry Using MALDI Biotyper, and Conventional Phenotypic Methods: a Comparison
title_full_unstemmed	Identification of Clinical Isolates of Alpha-Hemolytic Streptococci by 16S rRNA Gene Sequencing, Matrix-Assisted Laser Desorption Ionization-Time of Flight Mass Spectrometry Using MALDI Biotyper, and Conventional Phenotypic Methods: a Comparison
title_sort	Identification of Clinical Isolates of Alpha-Hemolytic Streptococci by 16S rRNA Gene Sequencing, Matrix-Assisted Laser Desorption Ionization-Time of Flight Mass Spectrometry Using MALDI Biotyper, and Conventional Phenotypic Methods: a Comparison
author_id_str_mv	f3806e5ff186dc9a67518a780ebff675 62586459693e05b2e1063967e76883f1 dc70f9d4badbbdb5d467fd321986d173
author_id_fullname_str_mv	f3806e5ff186dc9a67518a780ebff675_*_Jane Mikhail 62586459693e05b2e1063967e76883f1__Angharad Davies dc70f9d4badbbdb5d467fd321986d173_**_Llinos Harris
author	Jane Mikhail Angharad Davies Llinos Harris
author2	A. P Davies M Reid S. J Hadfield S Johnston J Mikhail L. G Harris H. F Jenkinson N Berry A. M Lewis K El-Bouri D Mack Jane Mikhail Angharad Davies Llinos Harris
format	Journal article
container_title	Journal of Clinical Microbiology
container_volume	50
container_issue	12
container_start_page	4087
publishDate	2012
institution	Swansea University
issn	0095-1137
doi_str_mv	10.1128/JCM.02387-12
college_str	Faculty of Medicine, Health and Life Sciences
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hierarchy_top_id	facultyofmedicinehealthandlifesciences
hierarchy_top_title	Faculty of Medicine, Health and Life Sciences
hierarchy_parent_id	facultyofmedicinehealthandlifesciences
hierarchy_parent_title	Faculty of Medicine, Health and Life Sciences
department_str	Swansea University Medical School - Medicine{{{_:::_}}}Faculty of Medicine, Health and Life Sciences{{{_:::_}}}Swansea University Medical School - Medicine
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description	Fifty-six α-haemolytic streptococcal isolates were identified using MALDI Biotyper MS (Bruker Daltonics), API 20 Strep (bioMerieux) and BD Phoenix (Becton Dickinson). Gold-standard identification was 16S rRNA gene sequence analysis with 16S-23S rRNA intergenic spacer sequencing. MALDI Biotyper correctly speciated 43% of isolates,, BD Phoenix 35% and API 20 Strep 26%.
published_date	2012-12-31T03:14:42Z
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score	11.012678

Identification of Clinical Isolates of Alpha-Hemolytic Streptococci by 16S rRNA Gene Sequencing, Matrix-Assisted Laser Desorption Ionization-Time of Flight Mass Spectrometry Using MALDI Biotyper, and Conventional Phenotypic Method...

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