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Analysis of oxysterol metabolomes / William, Griffiths

Biochimica et Biophysica Acta (BBA) - Molecular and Cell Biology of Lipids, Volume: 1811, Issue: 11, Pages: 784 - 799

Swansea University Author: William, Griffiths

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Abstract

Oxysterols are oxygenated forms of cholesterol. This definition can, however, be expanded toinclude oxygenated derivatives of plant sterols and also of cholesterol precursors. Oxysterols areformed in the first steps of cholesterol metabolism and also from cholesterol by reactive oxygenspecies. Oxyst...

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Published in: Biochimica et Biophysica Acta (BBA) - Molecular and Cell Biology of Lipids
ISSN: 1388-1981
Published: 2011
Online Access: Check full text

URI: https://cronfa.swan.ac.uk/Record/cronfa10758
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Abstract: Oxysterols are oxygenated forms of cholesterol. This definition can, however, be expanded toinclude oxygenated derivatives of plant sterols and also of cholesterol precursors. Oxysterols areformed in the first steps of cholesterol metabolism and also from cholesterol by reactive oxygenspecies. Oxysterols were once thought of as simple intermediates, or side-products, in the conversionof cholesterol to hormonal steroids and bile acids, however, they have subsequently been shown to bebiologically active molecules in their own right. In this article we will discuss methods of oxysterolanalysis including "classical" gas chromatography - mass spectrometry (GC-MS) methods and morerecent liquid chromatography (LC)-MS methods. Our main focus, however, will be on analyticalmethods based on "charge-tagging" and LC-tandem mass spectrometry (MS/MS or MSn) which wehave developed over the last decade in our laboratory. Examples will be given of oxysterol analysis inbrain, cerebrospinal fluid (CSF) and blood. The advantages and disadvantages of the various methodsof oxysterol analysis will be discussed.
College: Swansea University Medical School
Issue: 11
Start Page: 784
End Page: 799