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Analysis of oxysterol metabolomes / William Griffiths, Yuqin Wang

Biochimica et Biophysica Acta (BBA) - Molecular and Cell Biology of Lipids, Volume: 1811, Issue: 11, Pages: 784 - 799

Swansea University Author: William Griffiths

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Abstract

Oxysterols are oxygenated forms of cholesterol. This definition can, however, be expanded toinclude oxygenated derivatives of plant sterols and also of cholesterol precursors. Oxysterols areformed in the first steps of cholesterol metabolism and also from cholesterol by reactive oxygenspecies. Oxyst...

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Published in: Biochimica et Biophysica Acta (BBA) - Molecular and Cell Biology of Lipids
ISSN: 1388-1981
Published: 2011
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URI: https://cronfa.swan.ac.uk/Record/cronfa10758
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spelling 2011-10-01T00:00:00.0000000 v2 10758 2012-05-21 Analysis of oxysterol metabolomes 3316b1d1b524be1831790933eed1c26e 0000-0002-4129-6616 William Griffiths William Griffiths true false 2012-05-21 BMS Oxysterols are oxygenated forms of cholesterol. This definition can, however, be expanded toinclude oxygenated derivatives of plant sterols and also of cholesterol precursors. Oxysterols areformed in the first steps of cholesterol metabolism and also from cholesterol by reactive oxygenspecies. Oxysterols were once thought of as simple intermediates, or side-products, in the conversionof cholesterol to hormonal steroids and bile acids, however, they have subsequently been shown to bebiologically active molecules in their own right. In this article we will discuss methods of oxysterolanalysis including "classical" gas chromatography - mass spectrometry (GC-MS) methods and morerecent liquid chromatography (LC)-MS methods. Our main focus, however, will be on analyticalmethods based on "charge-tagging" and LC-tandem mass spectrometry (MS/MS or MSn) which wehave developed over the last decade in our laboratory. Examples will be given of oxysterol analysis inbrain, cerebrospinal fluid (CSF) and blood. The advantages and disadvantages of the various methodsof oxysterol analysis will be discussed. Other Biochimica et Biophysica Acta (BBA) - Molecular and Cell Biology of Lipids 1811 11 784 799 1388-1981 31 12 2011 2011-12-31 10.1016/j.bbalip.2011.05.012 COLLEGE NANME Biomedical Sciences COLLEGE CODE BMS Swansea University 2011-10-01T00:00:00.0000000 2012-05-21T15:33:37.8779609 Swansea University Medical School Medicine William Griffiths 0000-0002-4129-6616 1 Yuqin Wang 2
title Analysis of oxysterol metabolomes
spellingShingle Analysis of oxysterol metabolomes
William, Griffiths
title_short Analysis of oxysterol metabolomes
title_full Analysis of oxysterol metabolomes
title_fullStr Analysis of oxysterol metabolomes
title_full_unstemmed Analysis of oxysterol metabolomes
title_sort Analysis of oxysterol metabolomes
author_id_str_mv 3316b1d1b524be1831790933eed1c26e
author_id_fullname_str_mv 3316b1d1b524be1831790933eed1c26e_***_William, Griffiths
author William, Griffiths
author2 William Griffiths
Yuqin Wang
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container_title Biochimica et Biophysica Acta (BBA) - Molecular and Cell Biology of Lipids
container_volume 1811
container_issue 11
container_start_page 784
publishDate 2011
institution Swansea University
issn 1388-1981
doi_str_mv 10.1016/j.bbalip.2011.05.012
college_str Swansea University Medical School
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hierarchy_top_title Swansea University Medical School
hierarchy_parent_id swanseauniversitymedicalschool
hierarchy_parent_title Swansea University Medical School
department_str Medicine{{{_:::_}}}Swansea University Medical School{{{_:::_}}}Medicine
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description Oxysterols are oxygenated forms of cholesterol. This definition can, however, be expanded toinclude oxygenated derivatives of plant sterols and also of cholesterol precursors. Oxysterols areformed in the first steps of cholesterol metabolism and also from cholesterol by reactive oxygenspecies. Oxysterols were once thought of as simple intermediates, or side-products, in the conversionof cholesterol to hormonal steroids and bile acids, however, they have subsequently been shown to bebiologically active molecules in their own right. In this article we will discuss methods of oxysterolanalysis including "classical" gas chromatography - mass spectrometry (GC-MS) methods and morerecent liquid chromatography (LC)-MS methods. Our main focus, however, will be on analyticalmethods based on "charge-tagging" and LC-tandem mass spectrometry (MS/MS or MSn) which wehave developed over the last decade in our laboratory. Examples will be given of oxysterol analysis inbrain, cerebrospinal fluid (CSF) and blood. The advantages and disadvantages of the various methodsof oxysterol analysis will be discussed.
published_date 2011-12-31T03:21:47Z
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