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Analysis of oxysterol metabolomes

William Griffiths Orcid Logo, Yuqin Wang

Biochimica et Biophysica Acta (BBA) - Molecular and Cell Biology of Lipids, Volume: 1811, Issue: 11, Pages: 784 - 799

Swansea University Author: William Griffiths Orcid Logo

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DOI (Published version): 10.1016/j.bbalip.2011.05.012

Abstract

Oxysterols are oxygenated forms of cholesterol. This definition can, however, be expanded toinclude oxygenated derivatives of plant sterols and also of cholesterol precursors. Oxysterols areformed in the first steps of cholesterol metabolism and also from cholesterol by reactive oxygenspecies. Oxyst...

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Published in: Biochimica et Biophysica Acta (BBA) - Molecular and Cell Biology of Lipids
ISSN: 1388-1981
Published: 2011
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URI: https://cronfa.swan.ac.uk/Record/cronfa10758
first_indexed 2013-07-23T12:04:13Z
last_indexed 2018-02-09T04:39:51Z
id cronfa10758
recordtype SURis
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spelling 2011-10-01T00:00:00.0000000 v2 10758 2012-05-21 Analysis of oxysterol metabolomes 3316b1d1b524be1831790933eed1c26e 0000-0002-4129-6616 William Griffiths William Griffiths true false 2012-05-21 MEDS Oxysterols are oxygenated forms of cholesterol. This definition can, however, be expanded toinclude oxygenated derivatives of plant sterols and also of cholesterol precursors. Oxysterols areformed in the first steps of cholesterol metabolism and also from cholesterol by reactive oxygenspecies. Oxysterols were once thought of as simple intermediates, or side-products, in the conversionof cholesterol to hormonal steroids and bile acids, however, they have subsequently been shown to bebiologically active molecules in their own right. In this article we will discuss methods of oxysterolanalysis including "classical" gas chromatography - mass spectrometry (GC-MS) methods and morerecent liquid chromatography (LC)-MS methods. Our main focus, however, will be on analyticalmethods based on "charge-tagging" and LC-tandem mass spectrometry (MS/MS or MSn) which wehave developed over the last decade in our laboratory. Examples will be given of oxysterol analysis inbrain, cerebrospinal fluid (CSF) and blood. The advantages and disadvantages of the various methodsof oxysterol analysis will be discussed. Other Biochimica et Biophysica Acta (BBA) - Molecular and Cell Biology of Lipids 1811 11 784 799 1388-1981 31 12 2011 2011-12-31 10.1016/j.bbalip.2011.05.012 COLLEGE NANME Medical School COLLEGE CODE MEDS Swansea University 2011-10-01T00:00:00.0000000 2012-05-21T15:33:37.8779609 Faculty of Medicine, Health and Life Sciences Swansea University Medical School - Medicine William Griffiths 0000-0002-4129-6616 1 Yuqin Wang 2
title Analysis of oxysterol metabolomes
spellingShingle Analysis of oxysterol metabolomes
William Griffiths
title_short Analysis of oxysterol metabolomes
title_full Analysis of oxysterol metabolomes
title_fullStr Analysis of oxysterol metabolomes
title_full_unstemmed Analysis of oxysterol metabolomes
title_sort Analysis of oxysterol metabolomes
author_id_str_mv 3316b1d1b524be1831790933eed1c26e
author_id_fullname_str_mv 3316b1d1b524be1831790933eed1c26e_***_William Griffiths
author William Griffiths
author2 William Griffiths
Yuqin Wang
format Other
container_title Biochimica et Biophysica Acta (BBA) - Molecular and Cell Biology of Lipids
container_volume 1811
container_issue 11
container_start_page 784
publishDate 2011
institution Swansea University
issn 1388-1981
doi_str_mv 10.1016/j.bbalip.2011.05.012
college_str Faculty of Medicine, Health and Life Sciences
hierarchytype
hierarchy_top_id facultyofmedicinehealthandlifesciences
hierarchy_top_title Faculty of Medicine, Health and Life Sciences
hierarchy_parent_id facultyofmedicinehealthandlifesciences
hierarchy_parent_title Faculty of Medicine, Health and Life Sciences
department_str Swansea University Medical School - Medicine{{{_:::_}}}Faculty of Medicine, Health and Life Sciences{{{_:::_}}}Swansea University Medical School - Medicine
document_store_str 0
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description Oxysterols are oxygenated forms of cholesterol. This definition can, however, be expanded toinclude oxygenated derivatives of plant sterols and also of cholesterol precursors. Oxysterols areformed in the first steps of cholesterol metabolism and also from cholesterol by reactive oxygenspecies. Oxysterols were once thought of as simple intermediates, or side-products, in the conversionof cholesterol to hormonal steroids and bile acids, however, they have subsequently been shown to bebiologically active molecules in their own right. In this article we will discuss methods of oxysterolanalysis including "classical" gas chromatography - mass spectrometry (GC-MS) methods and morerecent liquid chromatography (LC)-MS methods. Our main focus, however, will be on analyticalmethods based on "charge-tagging" and LC-tandem mass spectrometry (MS/MS or MSn) which wehave developed over the last decade in our laboratory. Examples will be given of oxysterol analysis inbrain, cerebrospinal fluid (CSF) and blood. The advantages and disadvantages of the various methodsof oxysterol analysis will be discussed.
published_date 2011-12-31T18:22:05Z
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