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Bile acids: analysis in biological fluids and tissues

W. J Griffiths, J Sjovall, William Griffiths Orcid Logo

The Journal of Lipid Research, Volume: 51, Issue: 1, Pages: 23 - 41

Swansea University Author: William Griffiths Orcid Logo

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DOI (Published version): 10.1194/jlr.R001941-JLR200

Abstract

The formation of bile acids/bile alcohols is of major importance for the maintenance of cholesterol homeostasis. Besides their functions in lipid absorption, bile acids/bile alcohols are regulatory molecules for a number of metabolic processes. Their effects are structure-dependent, and numerous met...

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Published in: The Journal of Lipid Research
ISSN: 0022-2275
Published: 2010
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URI: https://cronfa.swan.ac.uk/Record/cronfa10783
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spelling 2011-10-01T00:00:00.0000000 v2 10783 2012-05-22 Bile acids: analysis in biological fluids and tissues 3316b1d1b524be1831790933eed1c26e 0000-0002-4129-6616 William Griffiths William Griffiths true false 2012-05-22 BMS The formation of bile acids/bile alcohols is of major importance for the maintenance of cholesterol homeostasis. Besides their functions in lipid absorption, bile acids/bile alcohols are regulatory molecules for a number of metabolic processes. Their effects are structure-dependent, and numerous metabolic conversions result in a complex mixture of biologically active and inactive forms. Advanced methods are required to characterize and quantify individual bile acids in these mixtures. A combination of such analyses with analyses of the proteome will be required for a better understanding of mechanisms of action and nature of endogenous ligands. Mass spectrometry is the basic detection technique for effluents from chromatographic columns. Capillary liquid chromatography-mass spectrometry with electrospray ionization provides the highest sensitivity in metabolome analysis. Classical gas chromatography-mass spectrometry is less sensitive but offers extensive structure-dependent fragmentation increasing the specificity in analyses of isobaric isomers of unconjugated bile acids. Depending on the nature of the bile acid/bile alcohol mixture and the range of concentration of individuals, different sample preparation sequences, from simple extractions to group separations and derivatizations, are applicable. We review the methods currently available for the analysis of bile acids in biological fluids and tissues, with emphasis on the combination of liquid and gas phase chromatography with mass spectrometry Other The Journal of Lipid Research 51 1 23 41 0022-2275 31 12 2010 2010-12-31 10.1194/jlr.R001941-JLR200 COLLEGE NANME Biomedical Sciences COLLEGE CODE BMS Swansea University 2011-10-01T00:00:00.0000000 2012-05-22T16:36:21.8377293 Faculty of Medicine, Health and Life Sciences Swansea University Medical School - Medicine W. J Griffiths 1 J Sjovall 2 William Griffiths 0000-0002-4129-6616 3
title Bile acids: analysis in biological fluids and tissues
spellingShingle Bile acids: analysis in biological fluids and tissues
William Griffiths
title_short Bile acids: analysis in biological fluids and tissues
title_full Bile acids: analysis in biological fluids and tissues
title_fullStr Bile acids: analysis in biological fluids and tissues
title_full_unstemmed Bile acids: analysis in biological fluids and tissues
title_sort Bile acids: analysis in biological fluids and tissues
author_id_str_mv 3316b1d1b524be1831790933eed1c26e
author_id_fullname_str_mv 3316b1d1b524be1831790933eed1c26e_***_William Griffiths
author William Griffiths
author2 W. J Griffiths
J Sjovall
William Griffiths
format Other
container_title The Journal of Lipid Research
container_volume 51
container_issue 1
container_start_page 23
publishDate 2010
institution Swansea University
issn 0022-2275
doi_str_mv 10.1194/jlr.R001941-JLR200
college_str Faculty of Medicine, Health and Life Sciences
hierarchytype
hierarchy_top_id facultyofmedicinehealthandlifesciences
hierarchy_top_title Faculty of Medicine, Health and Life Sciences
hierarchy_parent_id facultyofmedicinehealthandlifesciences
hierarchy_parent_title Faculty of Medicine, Health and Life Sciences
department_str Swansea University Medical School - Medicine{{{_:::_}}}Faculty of Medicine, Health and Life Sciences{{{_:::_}}}Swansea University Medical School - Medicine
document_store_str 0
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description The formation of bile acids/bile alcohols is of major importance for the maintenance of cholesterol homeostasis. Besides their functions in lipid absorption, bile acids/bile alcohols are regulatory molecules for a number of metabolic processes. Their effects are structure-dependent, and numerous metabolic conversions result in a complex mixture of biologically active and inactive forms. Advanced methods are required to characterize and quantify individual bile acids in these mixtures. A combination of such analyses with analyses of the proteome will be required for a better understanding of mechanisms of action and nature of endogenous ligands. Mass spectrometry is the basic detection technique for effluents from chromatographic columns. Capillary liquid chromatography-mass spectrometry with electrospray ionization provides the highest sensitivity in metabolome analysis. Classical gas chromatography-mass spectrometry is less sensitive but offers extensive structure-dependent fragmentation increasing the specificity in analyses of isobaric isomers of unconjugated bile acids. Depending on the nature of the bile acid/bile alcohol mixture and the range of concentration of individuals, different sample preparation sequences, from simple extractions to group separations and derivatizations, are applicable. We review the methods currently available for the analysis of bile acids in biological fluids and tissues, with emphasis on the combination of liquid and gas phase chromatography with mass spectrometry
published_date 2010-12-31T03:12:16Z
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score 10.999161

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