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Explants of Intact Endometrium to Model Bovine Innate Immunity and Inflammation Ex Vivo / Álan Maia Borges; Gareth David Healey; Iain Martin Sheldon

American Journal of Reproductive Immunology, Volume: 67, Issue: 6, Pages: 526 - 539

Swansea University Author: Sheldon, Martin

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Abstract

ProblemBacterial infections commonly cause bovine endometritis and infertility via innate immune pathways. However, mechanistic studies using isolated cells or chopped tissue may be compromised by the disruption of endometrial architecture and release of damage-associated molecular patterns. So, thi...

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Published in: American Journal of Reproductive Immunology
ISSN: 1046-7408
Published: 2012
Online Access: Check full text

URI: https://cronfa.swan.ac.uk/Record/cronfa11549
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Abstract: ProblemBacterial infections commonly cause bovine endometritis and infertility via innate immune pathways. However, mechanistic studies using isolated cells or chopped tissue may be compromised by the disruption of endometrial architecture and release of damage-associated molecular patterns. So, this study aimed to establish an ex vivo model of intact bovine endometrium to study innate immunity and inflammation.Method of studyIntact bovine endometrium explants were collected using a sterile 8-mm punch biopsy and cultured ex vivo with bacteria or pathogen-associated molecules. Interleukin accumulation was measured, and tissue viability was assessed by microscopy, TdT-mediated biotin–dUTP nick-end labelling and lactate dehydrogenase assay.ResultsIntact endometrium explants accumulated IL-6, IL-1β and IL-8 in response to Gram-negative or Gram-positive bacteria, and their purified pathogen-associated molecules; inflammatory responses were dependent on the stage of oestrous cycle. Explants of intact endometrium maintained viability and tissue architecture, and had lower basal accumulation of interleukins compared with explants using chopped endometrium.ConclusionThis study established a tractable ex vivo model of intact endometrium to explore the mechanisms of immunity and inflammation in the bovine endometrium.
College: Swansea University Medical School
Issue: 6
Start Page: 526
End Page: 539