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Explants of Intact Endometrium to Model Bovine Innate Immunity and Inflammation Ex Vivo

Álan Maia Borges, Gareth Healey Orcid Logo, Iain Martin Sheldon, Martin Sheldon Orcid Logo

American Journal of Reproductive Immunology, Volume: 67, Issue: 6, Pages: 526 - 539

Swansea University Authors: Gareth Healey Orcid Logo, Martin Sheldon Orcid Logo

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DOI (Published version): 10.1111/j.1600-0897.2012.01106.x

Abstract

ProblemBacterial infections commonly cause bovine endometritis and infertility via innate immune pathways. However, mechanistic studies using isolated cells or chopped tissue may be compromised by the disruption of endometrial architecture and release of damage-associated molecular patterns. So, thi...

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Published in: American Journal of Reproductive Immunology
ISSN: 1046-7408
Published: 2012
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URI: https://cronfa.swan.ac.uk/Record/cronfa11549
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first_indexed 2013-07-23T12:05:47Z
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spelling 2011-10-01T00:00:00.0000000 v2 11549 2012-06-14 Explants of Intact Endometrium to Model Bovine Innate Immunity and Inflammation Ex Vivo 5926519f89187489cfd5e1478aa188b1 0000-0001-9531-1220 Gareth Healey Gareth Healey true false ab0f74b794e59cc270c69e63ee1d9748 0000-0001-7902-5558 Martin Sheldon Martin Sheldon true false 2012-06-14 PMSC ProblemBacterial infections commonly cause bovine endometritis and infertility via innate immune pathways. However, mechanistic studies using isolated cells or chopped tissue may be compromised by the disruption of endometrial architecture and release of damage-associated molecular patterns. So, this study aimed to establish an ex vivo model of intact bovine endometrium to study innate immunity and inflammation.Method of studyIntact bovine endometrium explants were collected using a sterile 8-mm punch biopsy and cultured ex vivo with bacteria or pathogen-associated molecules. Interleukin accumulation was measured, and tissue viability was assessed by microscopy, TdT-mediated biotin–dUTP nick-end labelling and lactate dehydrogenase assay.ResultsIntact endometrium explants accumulated IL-6, IL-1β and IL-8 in response to Gram-negative or Gram-positive bacteria, and their purified pathogen-associated molecules; inflammatory responses were dependent on the stage of oestrous cycle. Explants of intact endometrium maintained viability and tissue architecture, and had lower basal accumulation of interleukins compared with explants using chopped endometrium.ConclusionThis study established a tractable ex vivo model of intact endometrium to explore the mechanisms of immunity and inflammation in the bovine endometrium. Journal Article American Journal of Reproductive Immunology 67 6 526 539 1046-7408 31 12 2012 2012-12-31 10.1111/j.1600-0897.2012.01106.x COLLEGE NANME Medicine COLLEGE CODE PMSC Swansea University 2011-10-01T00:00:00.0000000 2012-06-14T15:40:34.1134013 Faculty of Medicine, Health and Life Sciences Swansea University Medical School - Medicine Álan Maia Borges 1 Gareth Healey 0000-0001-9531-1220 2 Iain Martin Sheldon 3 Martin Sheldon 0000-0001-7902-5558 4
title Explants of Intact Endometrium to Model Bovine Innate Immunity and Inflammation Ex Vivo
spellingShingle Explants of Intact Endometrium to Model Bovine Innate Immunity and Inflammation Ex Vivo
Gareth Healey
Martin Sheldon
title_short Explants of Intact Endometrium to Model Bovine Innate Immunity and Inflammation Ex Vivo
title_full Explants of Intact Endometrium to Model Bovine Innate Immunity and Inflammation Ex Vivo
title_fullStr Explants of Intact Endometrium to Model Bovine Innate Immunity and Inflammation Ex Vivo
title_full_unstemmed Explants of Intact Endometrium to Model Bovine Innate Immunity and Inflammation Ex Vivo
title_sort Explants of Intact Endometrium to Model Bovine Innate Immunity and Inflammation Ex Vivo
author_id_str_mv 5926519f89187489cfd5e1478aa188b1
ab0f74b794e59cc270c69e63ee1d9748
author_id_fullname_str_mv 5926519f89187489cfd5e1478aa188b1_***_Gareth Healey
ab0f74b794e59cc270c69e63ee1d9748_***_Martin Sheldon
author Gareth Healey
Martin Sheldon
author2 Álan Maia Borges
Gareth Healey
Iain Martin Sheldon
Martin Sheldon
format Journal article
container_title American Journal of Reproductive Immunology
container_volume 67
container_issue 6
container_start_page 526
publishDate 2012
institution Swansea University
issn 1046-7408
doi_str_mv 10.1111/j.1600-0897.2012.01106.x
college_str Faculty of Medicine, Health and Life Sciences
hierarchytype
hierarchy_top_id facultyofmedicinehealthandlifesciences
hierarchy_top_title Faculty of Medicine, Health and Life Sciences
hierarchy_parent_id facultyofmedicinehealthandlifesciences
hierarchy_parent_title Faculty of Medicine, Health and Life Sciences
department_str Swansea University Medical School - Medicine{{{_:::_}}}Faculty of Medicine, Health and Life Sciences{{{_:::_}}}Swansea University Medical School - Medicine
document_store_str 0
active_str 0
description ProblemBacterial infections commonly cause bovine endometritis and infertility via innate immune pathways. However, mechanistic studies using isolated cells or chopped tissue may be compromised by the disruption of endometrial architecture and release of damage-associated molecular patterns. So, this study aimed to establish an ex vivo model of intact bovine endometrium to study innate immunity and inflammation.Method of studyIntact bovine endometrium explants were collected using a sterile 8-mm punch biopsy and cultured ex vivo with bacteria or pathogen-associated molecules. Interleukin accumulation was measured, and tissue viability was assessed by microscopy, TdT-mediated biotin–dUTP nick-end labelling and lactate dehydrogenase assay.ResultsIntact endometrium explants accumulated IL-6, IL-1β and IL-8 in response to Gram-negative or Gram-positive bacteria, and their purified pathogen-associated molecules; inflammatory responses were dependent on the stage of oestrous cycle. Explants of intact endometrium maintained viability and tissue architecture, and had lower basal accumulation of interleukins compared with explants using chopped endometrium.ConclusionThis study established a tractable ex vivo model of intact endometrium to explore the mechanisms of immunity and inflammation in the bovine endometrium.
published_date 2012-12-31T03:13:23Z
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score 11.016235

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