No Cover Image

Journal article 361 views 74 downloads

Short communication: Glutamine modulates inflammatory responses to lipopolysaccharide in ex vivo bovine endometrium / Martin, Sheldon

Journal of Dairy Science, Volume: 100, Pages: 2207 - 2212

Swansea University Author: Martin, Sheldon

  • JDSNoletov2.pdf

    PDF | Version of Record

    This is an open access article under the CC BY 2.0 license (http://creativecommons.org/licenses/by/2.0/).

    Download (470.96KB)

Check full text

DOI (Published version): 10.3168/jds.2016-12023

Abstract

Bacteria infect the endometrium lining the uterus of cattle after parturition, and clearance of these microbes depends on a robust innate immune response to bacterial molecules, such as the endotoxin lipopolysaccharide (LPS). Endometrial inflammation is characterized by secretion of the cytokines IL...

Full description

Published in: Journal of Dairy Science
ISSN: 00220302
Published: 2017
Online Access: Check full text

URI: https://cronfa.swan.ac.uk/Record/cronfa31648
Tags: Add Tag
No Tags, Be the first to tag this record!
Abstract: Bacteria infect the endometrium lining the uterus of cattle after parturition, and clearance of these microbes depends on a robust innate immune response to bacterial molecules, such as the endotoxin lipopolysaccharide (LPS). Endometrial inflammation is characterized by secretion of the cytokines IL-1β and IL-6 and the chemokine IL-8. However, animals often fail to clear invading bacteria and develop uterine disease if they are in negative energy balance, with reduced abundance of glucose and glutamine, which are substrates for energy in tissues. Depletion of glucose blunts inflammatory responses in the endometrium, but the role of glutamine is not clear. The present study tested the hypothesis that depletion of glutamine compromises inflammatory responses to LPS in endometrial tissue. Ex vivo organ cultures of endometrium were challenged with LPS, and culture supernatants accumulated IL-1β, IL-6, and IL-8, as expected. However, reducing the availability of glutamine in culture medium containing glucose reduced the accumulation of IL-1β, IL-6, and IL-8 by >50%. Surprisingly, in the absence of glucose, supplying increasing amounts of glutamine was not sufficient to augment inflammatory responses to LPS, whereas, in the absence of glutamine, supplying more glucose increased inflammation. Furthermore, inhibiting glycolysis reduced the accumulation of IL-1β, IL-6, and IL-8 by >50%, even when glutamine and glucose were abundant. In conclusion, depletion of glutamine reduces inflammatory responses to LPS in the endometrium, and the activity of glutamine depends on glucose and glycolysis. These data provide mechanistic insights into how negative energy balance may be linked to postpartum uterine disease.
Item Description: The work was funded by a project grant to IMS bythe Biotechnology and Biological Sciences ResearchCouncil (BB/I017240/1; Swindon, UK). Coordenacaode Aperfeicoamento de Pessoal de Nivel Superior(99999.010219/2014-05; Brasilia, Brazil) and ConselhoNacional de Desenvolvimento Cientifico e Tecnologico(486143/2013-9; Brasilia, Brazil) were supported PGNand JPES.
College: Swansea University Medical School
Start Page: 2207
End Page: 2212