No Cover Image

Journal article 1305 views

Dormant Cells of Staphylococcus aureus Are Resuscitated by Spent Culture Supernatant

Ben Pascoe Orcid Logo, Lucy Dams, Thomas Wilkinson Orcid Logo, Llinos Harris Orcid Logo, Owen Bodger Orcid Logo, Dietrich Mack, Angharad Davies Orcid Logo

PLoS ONE, Volume: 9, Issue: 2, Start page: e85998

Swansea University Authors: Ben Pascoe Orcid Logo, Thomas Wilkinson Orcid Logo, Llinos Harris Orcid Logo, Owen Bodger Orcid Logo, Angharad Davies Orcid Logo

Full text not available from this repository: check for access using links below.

DOI (Published version): 10.1371/journal.pone.0085998

Abstract

We describe the first in vitro model of dormancy in Staphylococcus aureus, showing that cells are generated which can beresuscitated by addition of spent medium supernatant taken from cultures of the same organism. Over 30 days, culturablecounts in dormant cultures of S. aureus SH1000 fell from 106–...

Full description

Published in: PLoS ONE
Published: 2014
Online Access: http://www.plosone.org/article/info%3Adoi%2F10.1371%2Fjournal.pone.0085998
URI: https://cronfa.swan.ac.uk/Record/cronfa17301
Tags: Add Tag
No Tags, Be the first to tag this record!
first_indexed 2014-02-12T03:06:10Z
last_indexed 2018-02-09T04:50:49Z
id cronfa17301
recordtype SURis
fullrecord <?xml version="1.0"?><rfc1807><datestamp>2016-04-19T23:51:39.3168921</datestamp><bib-version>v2</bib-version><id>17301</id><entry>2014-02-11</entry><title>Dormant Cells of Staphylococcus aureus Are Resuscitated by Spent Culture Supernatant</title><swanseaauthors><author><sid>4660c0eb7e6bfd796cd749ae713ea558</sid><ORCID>0000-0001-6376-5121</ORCID><firstname>Ben</firstname><surname>Pascoe</surname><name>Ben Pascoe</name><active>true</active><ethesisStudent>false</ethesisStudent></author><author><sid>86cca6bf31bfe8572de27c1b441420d8</sid><ORCID>0000-0003-0397-6079</ORCID><firstname>Thomas</firstname><surname>Wilkinson</surname><name>Thomas Wilkinson</name><active>true</active><ethesisStudent>false</ethesisStudent></author><author><sid>dc70f9d4badbbdb5d467fd321986d173</sid><ORCID>0000-0002-0295-3038</ORCID><firstname>Llinos</firstname><surname>Harris</surname><name>Llinos Harris</name><active>true</active><ethesisStudent>false</ethesisStudent></author><author><sid>8096440ab42b60a86e6aba678fe2695a</sid><ORCID>0000-0002-4022-9964</ORCID><firstname>Owen</firstname><surname>Bodger</surname><name>Owen Bodger</name><active>true</active><ethesisStudent>false</ethesisStudent></author><author><sid>62586459693e05b2e1063967e76883f1</sid><ORCID>0000-0003-4190-8894</ORCID><firstname>Angharad</firstname><surname>Davies</surname><name>Angharad Davies</name><active>true</active><ethesisStudent>false</ethesisStudent></author></swanseaauthors><date>2014-02-11</date><deptcode>PMSC</deptcode><abstract>We describe the first in vitro model of dormancy in Staphylococcus aureus, showing that cells are generated which can beresuscitated by addition of spent medium supernatant taken from cultures of the same organism. Over 30 days, culturablecounts in dormant cultures of S. aureus SH1000 fell from 106&#x2013;107 cfu/ml to ,10 cfu/ml as measured by the Most ProbableNumber method in liquid culture, while total counts as determined by microscopy, and supported by data from RT-qPCR,remained around 106&#x2013;107 cells/ml. Supplementing cultures with 25&#x2013;50% spent medium resulted in a .600-fold increase inbacterial growth. Resuscitation was a specific effect, greatly reduced by boiling or addition of trypsin to the spentsupernatant. Supernatant also effected a reduction in lag phase of dormant cultures. SEM demonstrated the presence ofsmall coccoid cells in dormant cultures. The results are similar to those seen with resuscitation promoting factors (Rpfs) inactinobacteria. This is the first time resuscitation has been demonstrated in Staphylococcus aureus, which is an importanthuman pathogen. A better understanding of control and reactivation of dormant cells could lead to major improvements inmanaging staphylococcal infections; resuscitation could be an important step in restoring susceptibility to antibiotictreatment.</abstract><type>Journal Article</type><journal>PLoS ONE</journal><volume>9</volume><journalNumber>2</journalNumber><paginationStart>e85998</paginationStart><publisher/><keywords>Staphylococcus aureus; dormancy; bacterial infection; chronic infection</keywords><publishedDay>11</publishedDay><publishedMonth>2</publishedMonth><publishedYear>2014</publishedYear><publishedDate>2014-02-11</publishedDate><doi>10.1371/journal.pone.0085998</doi><url>http://www.plosone.org/article/info%3Adoi%2F10.1371%2Fjournal.pone.0085998</url><notes></notes><college>COLLEGE NANME</college><department>Medicine</department><CollegeCode>COLLEGE CODE</CollegeCode><DepartmentCode>PMSC</DepartmentCode><institution>Swansea University</institution><apcterm/><lastEdited>2016-04-19T23:51:39.3168921</lastEdited><Created>2014-02-11T23:37:13.9807140</Created><path><level id="1">Faculty of Medicine, Health and Life Sciences</level><level id="2">Swansea University Medical School - Medicine</level></path><authors><author><firstname>Ben</firstname><surname>Pascoe</surname><orcid>0000-0001-6376-5121</orcid><order>1</order></author><author><firstname>Lucy</firstname><surname>Dams</surname><order>2</order></author><author><firstname>Thomas</firstname><surname>Wilkinson</surname><orcid>0000-0003-0397-6079</orcid><order>3</order></author><author><firstname>Llinos</firstname><surname>Harris</surname><orcid>0000-0002-0295-3038</orcid><order>4</order></author><author><firstname>Owen</firstname><surname>Bodger</surname><orcid>0000-0002-4022-9964</orcid><order>5</order></author><author><firstname>Dietrich</firstname><surname>Mack</surname><order>6</order></author><author><firstname>Angharad</firstname><surname>Davies</surname><orcid>0000-0003-4190-8894</orcid><order>7</order></author></authors><documents/><OutputDurs/></rfc1807>
spelling 2016-04-19T23:51:39.3168921 v2 17301 2014-02-11 Dormant Cells of Staphylococcus aureus Are Resuscitated by Spent Culture Supernatant 4660c0eb7e6bfd796cd749ae713ea558 0000-0001-6376-5121 Ben Pascoe Ben Pascoe true false 86cca6bf31bfe8572de27c1b441420d8 0000-0003-0397-6079 Thomas Wilkinson Thomas Wilkinson true false dc70f9d4badbbdb5d467fd321986d173 0000-0002-0295-3038 Llinos Harris Llinos Harris true false 8096440ab42b60a86e6aba678fe2695a 0000-0002-4022-9964 Owen Bodger Owen Bodger true false 62586459693e05b2e1063967e76883f1 0000-0003-4190-8894 Angharad Davies Angharad Davies true false 2014-02-11 PMSC We describe the first in vitro model of dormancy in Staphylococcus aureus, showing that cells are generated which can beresuscitated by addition of spent medium supernatant taken from cultures of the same organism. Over 30 days, culturablecounts in dormant cultures of S. aureus SH1000 fell from 106–107 cfu/ml to ,10 cfu/ml as measured by the Most ProbableNumber method in liquid culture, while total counts as determined by microscopy, and supported by data from RT-qPCR,remained around 106–107 cells/ml. Supplementing cultures with 25–50% spent medium resulted in a .600-fold increase inbacterial growth. Resuscitation was a specific effect, greatly reduced by boiling or addition of trypsin to the spentsupernatant. Supernatant also effected a reduction in lag phase of dormant cultures. SEM demonstrated the presence ofsmall coccoid cells in dormant cultures. The results are similar to those seen with resuscitation promoting factors (Rpfs) inactinobacteria. This is the first time resuscitation has been demonstrated in Staphylococcus aureus, which is an importanthuman pathogen. A better understanding of control and reactivation of dormant cells could lead to major improvements inmanaging staphylococcal infections; resuscitation could be an important step in restoring susceptibility to antibiotictreatment. Journal Article PLoS ONE 9 2 e85998 Staphylococcus aureus; dormancy; bacterial infection; chronic infection 11 2 2014 2014-02-11 10.1371/journal.pone.0085998 http://www.plosone.org/article/info%3Adoi%2F10.1371%2Fjournal.pone.0085998 COLLEGE NANME Medicine COLLEGE CODE PMSC Swansea University 2016-04-19T23:51:39.3168921 2014-02-11T23:37:13.9807140 Faculty of Medicine, Health and Life Sciences Swansea University Medical School - Medicine Ben Pascoe 0000-0001-6376-5121 1 Lucy Dams 2 Thomas Wilkinson 0000-0003-0397-6079 3 Llinos Harris 0000-0002-0295-3038 4 Owen Bodger 0000-0002-4022-9964 5 Dietrich Mack 6 Angharad Davies 0000-0003-4190-8894 7
title Dormant Cells of Staphylococcus aureus Are Resuscitated by Spent Culture Supernatant
spellingShingle Dormant Cells of Staphylococcus aureus Are Resuscitated by Spent Culture Supernatant
Ben Pascoe
Thomas Wilkinson
Llinos Harris
Owen Bodger
Angharad Davies
title_short Dormant Cells of Staphylococcus aureus Are Resuscitated by Spent Culture Supernatant
title_full Dormant Cells of Staphylococcus aureus Are Resuscitated by Spent Culture Supernatant
title_fullStr Dormant Cells of Staphylococcus aureus Are Resuscitated by Spent Culture Supernatant
title_full_unstemmed Dormant Cells of Staphylococcus aureus Are Resuscitated by Spent Culture Supernatant
title_sort Dormant Cells of Staphylococcus aureus Are Resuscitated by Spent Culture Supernatant
author_id_str_mv 4660c0eb7e6bfd796cd749ae713ea558
86cca6bf31bfe8572de27c1b441420d8
dc70f9d4badbbdb5d467fd321986d173
8096440ab42b60a86e6aba678fe2695a
62586459693e05b2e1063967e76883f1
author_id_fullname_str_mv 4660c0eb7e6bfd796cd749ae713ea558_***_Ben Pascoe
86cca6bf31bfe8572de27c1b441420d8_***_Thomas Wilkinson
dc70f9d4badbbdb5d467fd321986d173_***_Llinos Harris
8096440ab42b60a86e6aba678fe2695a_***_Owen Bodger
62586459693e05b2e1063967e76883f1_***_Angharad Davies
author Ben Pascoe
Thomas Wilkinson
Llinos Harris
Owen Bodger
Angharad Davies
author2 Ben Pascoe
Lucy Dams
Thomas Wilkinson
Llinos Harris
Owen Bodger
Dietrich Mack
Angharad Davies
format Journal article
container_title PLoS ONE
container_volume 9
container_issue 2
container_start_page e85998
publishDate 2014
institution Swansea University
doi_str_mv 10.1371/journal.pone.0085998
college_str Faculty of Medicine, Health and Life Sciences
hierarchytype
hierarchy_top_id facultyofmedicinehealthandlifesciences
hierarchy_top_title Faculty of Medicine, Health and Life Sciences
hierarchy_parent_id facultyofmedicinehealthandlifesciences
hierarchy_parent_title Faculty of Medicine, Health and Life Sciences
department_str Swansea University Medical School - Medicine{{{_:::_}}}Faculty of Medicine, Health and Life Sciences{{{_:::_}}}Swansea University Medical School - Medicine
url http://www.plosone.org/article/info%3Adoi%2F10.1371%2Fjournal.pone.0085998
document_store_str 0
active_str 0
description We describe the first in vitro model of dormancy in Staphylococcus aureus, showing that cells are generated which can beresuscitated by addition of spent medium supernatant taken from cultures of the same organism. Over 30 days, culturablecounts in dormant cultures of S. aureus SH1000 fell from 106–107 cfu/ml to ,10 cfu/ml as measured by the Most ProbableNumber method in liquid culture, while total counts as determined by microscopy, and supported by data from RT-qPCR,remained around 106–107 cells/ml. Supplementing cultures with 25–50% spent medium resulted in a .600-fold increase inbacterial growth. Resuscitation was a specific effect, greatly reduced by boiling or addition of trypsin to the spentsupernatant. Supernatant also effected a reduction in lag phase of dormant cultures. SEM demonstrated the presence ofsmall coccoid cells in dormant cultures. The results are similar to those seen with resuscitation promoting factors (Rpfs) inactinobacteria. This is the first time resuscitation has been demonstrated in Staphylococcus aureus, which is an importanthuman pathogen. A better understanding of control and reactivation of dormant cells could lead to major improvements inmanaging staphylococcal infections; resuscitation could be an important step in restoring susceptibility to antibiotictreatment.
published_date 2014-02-11T03:19:57Z
_version_ 1763750546967101440
score 11.017797

Similar Items