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A new derivative for oxosteroid analysis by mass spectrometry / William, Griffiths; Yuqin, Wang

Biochemical and Biophysical Research Communications

Swansesa University Authors: William, Griffiths, Yuqin, Wang

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DOI (Published version): 10.1016/j.bbrc.2014.01.190

Abstract

Here we report a new method for oxosteroid identification utilizing "tandem mass tag hydrazine" (TMTH) carbonyl-reactive derivatisation reagent. TMTH is a reagent with a chargeable tertiary amino group attached through a linker to a carbonyl-reactive hydrazine group. Thirty oxoster...

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Published in: Biochemical and Biophysical Research Communications
Published: 2014
URI: https://cronfa.swan.ac.uk/Record/cronfa17815
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Abstract: Here we report a new method for oxosteroid identification utilizing "tandem mass tag hydrazine" (TMTH) carbonyl-reactive derivatisation reagent. TMTH is a reagent with a chargeable tertiary amino group attached through a linker to a carbonyl-reactive hydrazine group. Thirty oxosteroids were analysed after derivatisation with TMTH by electrospray ionization mass spectrometry (ESI-MS) and were found to give high ion-currents compared to underivatised molecules. ESI-tandem mass spectrometry (MS/MS) analysis of the derivatives yielded characteristic fragmentation patterns with specific mass reporter ions derived from the TMT group. A shotgun ESI-MS method incorporating TMTH derivatisation was applied to a urine sample
College: Swansea University Medical School