Journal article 1127 views
A new derivative for oxosteroid analysis by mass spectrometry
Biochemical and Biophysical Research Communications
Swansea University Authors: William Griffiths , Yuqin Wang
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DOI (Published version): 10.1016/j.bbrc.2014.01.190
Abstract
Here we report a new method for oxosteroid identification utilizing "tandem mass tag hydrazine" (TMTH) carbonyl-reactive derivatisation reagent. TMTH is a reagent with a chargeable tertiary amino group attached through a linker to a carbonyl-reactive hydrazine group. Thirty oxosteroids wer...
Published in: | Biochemical and Biophysical Research Communications |
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2014
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URI: | https://cronfa.swan.ac.uk/Record/cronfa17815 |
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2014-04-15T01:30:03Z |
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2018-02-09T04:51:49Z |
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2014-04-14T16:41:01.6263853 v2 17815 2014-04-14 A new derivative for oxosteroid analysis by mass spectrometry 3316b1d1b524be1831790933eed1c26e 0000-0002-4129-6616 William Griffiths William Griffiths true false c92729b58622f9fdf6a0e7d8f4ce5081 0000-0002-3063-3066 Yuqin Wang Yuqin Wang true false 2014-04-14 MEDS Here we report a new method for oxosteroid identification utilizing "tandem mass tag hydrazine" (TMTH) carbonyl-reactive derivatisation reagent. TMTH is a reagent with a chargeable tertiary amino group attached through a linker to a carbonyl-reactive hydrazine group. Thirty oxosteroids were analysed after derivatisation with TMTH by electrospray ionization mass spectrometry (ESI-MS) and were found to give high ion-currents compared to underivatised molecules. ESI-tandem mass spectrometry (MS/MS) analysis of the derivatives yielded characteristic fragmentation patterns with specific mass reporter ions derived from the TMT group. A shotgun ESI-MS method incorporating TMTH derivatisation was applied to a urine sample Journal Article Biochemical and Biophysical Research Communications 31 12 2014 2014-12-31 10.1016/j.bbrc.2014.01.190 COLLEGE NANME Medical School COLLEGE CODE MEDS Swansea University 2014-04-14T16:41:01.6263853 2014-04-14T16:41:01.6263853 Faculty of Medicine, Health and Life Sciences Swansea University Medical School - Medicine 1 William Griffiths 0000-0002-4129-6616 2 Yuqin Wang 0000-0002-3063-3066 3 |
title |
A new derivative for oxosteroid analysis by mass spectrometry |
spellingShingle |
A new derivative for oxosteroid analysis by mass spectrometry William Griffiths Yuqin Wang |
title_short |
A new derivative for oxosteroid analysis by mass spectrometry |
title_full |
A new derivative for oxosteroid analysis by mass spectrometry |
title_fullStr |
A new derivative for oxosteroid analysis by mass spectrometry |
title_full_unstemmed |
A new derivative for oxosteroid analysis by mass spectrometry |
title_sort |
A new derivative for oxosteroid analysis by mass spectrometry |
author_id_str_mv |
3316b1d1b524be1831790933eed1c26e c92729b58622f9fdf6a0e7d8f4ce5081 |
author_id_fullname_str_mv |
3316b1d1b524be1831790933eed1c26e_***_William Griffiths c92729b58622f9fdf6a0e7d8f4ce5081_***_Yuqin Wang |
author |
William Griffiths Yuqin Wang |
author2 |
William Griffiths Yuqin Wang |
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Journal article |
container_title |
Biochemical and Biophysical Research Communications |
publishDate |
2014 |
institution |
Swansea University |
doi_str_mv |
10.1016/j.bbrc.2014.01.190 |
college_str |
Faculty of Medicine, Health and Life Sciences |
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|
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facultyofmedicinehealthandlifesciences |
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Faculty of Medicine, Health and Life Sciences |
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facultyofmedicinehealthandlifesciences |
hierarchy_parent_title |
Faculty of Medicine, Health and Life Sciences |
department_str |
Swansea University Medical School - Medicine{{{_:::_}}}Faculty of Medicine, Health and Life Sciences{{{_:::_}}}Swansea University Medical School - Medicine |
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description |
Here we report a new method for oxosteroid identification utilizing "tandem mass tag hydrazine" (TMTH) carbonyl-reactive derivatisation reagent. TMTH is a reagent with a chargeable tertiary amino group attached through a linker to a carbonyl-reactive hydrazine group. Thirty oxosteroids were analysed after derivatisation with TMTH by electrospray ionization mass spectrometry (ESI-MS) and were found to give high ion-currents compared to underivatised molecules. ESI-tandem mass spectrometry (MS/MS) analysis of the derivatives yielded characteristic fragmentation patterns with specific mass reporter ions derived from the TMT group. A shotgun ESI-MS method incorporating TMTH derivatisation was applied to a urine sample |
published_date |
2014-12-31T18:35:41Z |
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1822065785071206400 |
score |
11.048302 |