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Enhanced biofilm formation and multi-host transmission evolve from divergent genetic backgrounds inCampylobacter jejuni

Ben Pascoe Orcid Logo, Guillaume Méric, Susan Murray, Koji Yahara, Leonardos Mageiros, Ryan Bowen, Nathan H. Jones, Rose E. Jeeves, Hilary M. Lappin-Scott, Hiroshi Asakura, Samuel K. Sheppard, Guillaume Meric

Environmental Microbiology

Swansea University Authors: Ben Pascoe Orcid Logo, Guillaume Meric

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DOI (Published version): 10.1111/1462-2920.13051

Abstract

Multicellular biofilms are an ancient bacterial adaptation that can offer a protective environment for survival in hostile habitats. In microaerophilic organisms like Campylobacter, biofilms can play a key role in transmission to humans as the bacteria are exposed to atmospheric oxygen concentration...

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Published in: Environmental Microbiology
Published: 2015
Online Access: http://onlinelibrary.wiley.com/doi/10.1111/1462-2920.13051/abstract
URI: https://cronfa.swan.ac.uk/Record/cronfa18744
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first_indexed 2015-08-21T02:02:00Z
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spelling 2016-03-22T14:49:19.0984490 v2 18744 2014-10-16 Enhanced biofilm formation and multi-host transmission evolve from divergent genetic backgrounds inCampylobacter jejuni 4660c0eb7e6bfd796cd749ae713ea558 0000-0001-6376-5121 Ben Pascoe Ben Pascoe true false 9384e450ee619395be7459d6bd7a8f6d Guillaume Meric Guillaume Meric true false 2014-10-16 PMSC Multicellular biofilms are an ancient bacterial adaptation that can offer a protective environment for survival in hostile habitats. In microaerophilic organisms like Campylobacter, biofilms can play a key role in transmission to humans as the bacteria are exposed to atmospheric oxygen concentrations when leaving the reservoir host gut. The genetic determinants of biofilm formation can differ between species but little is known about how strains of the same species achieve the biofilm phenotype with different genetic backgrounds. We take an integrated genomics approach to combine genome-wide association studies with traditional microbiology techniques to investigate the genetic basis of biofilm formation in 102 Campylobacter jejuni isolates. The study quantified biofilm formation among the isolates and identified hotspots of genetic variation in homologous sequence that corresponded to variation in biofilm phenotypes. In total, 46 genes showed a statistically robust association including those involved in adhesion, motility, nitrosative and oxidative stress. Previously reported and novel genes, containing associated elements, clustered on the genome - including 9 multi-gene transcriptional units. This is consistent with genetic linkage and the horizontal acquisition of biofilm genes in recombining organisms such as C. jejuni. The genes associated with biofilm formation were different in the host generalist ST-21 and ST-45 clonal complexes, which are frequently isolated from multiple host species and clinical samples, but both had enhanced biofilm formation compared to host specialists. This suggests the evolution of enhanced biofilm from different genetic backgrounds and a possible role in colonisation of multiple hosts and transmission to humans. Journal Article Environmental Microbiology Campylobacter / Genome-wide association study / Transmission ecology / Oxidative stress / Biofilm formation / Host generalism 16 9 2015 2015-09-16 10.1111/1462-2920.13051 http://onlinelibrary.wiley.com/doi/10.1111/1462-2920.13051/abstract COLLEGE NANME Medicine COLLEGE CODE PMSC Swansea University 2016-03-22T14:49:19.0984490 2014-10-16T21:51:59.4316916 Faculty of Medicine, Health and Life Sciences Swansea University Medical School - Medicine Ben Pascoe 0000-0001-6376-5121 1 Guillaume Méric 2 Susan Murray 3 Koji Yahara 4 Leonardos Mageiros 5 Ryan Bowen 6 Nathan H. Jones 7 Rose E. Jeeves 8 Hilary M. Lappin-Scott 9 Hiroshi Asakura 10 Samuel K. Sheppard 11 Guillaume Meric 12
title Enhanced biofilm formation and multi-host transmission evolve from divergent genetic backgrounds inCampylobacter jejuni
spellingShingle Enhanced biofilm formation and multi-host transmission evolve from divergent genetic backgrounds inCampylobacter jejuni
Ben Pascoe
Guillaume Meric
title_short Enhanced biofilm formation and multi-host transmission evolve from divergent genetic backgrounds inCampylobacter jejuni
title_full Enhanced biofilm formation and multi-host transmission evolve from divergent genetic backgrounds inCampylobacter jejuni
title_fullStr Enhanced biofilm formation and multi-host transmission evolve from divergent genetic backgrounds inCampylobacter jejuni
title_full_unstemmed Enhanced biofilm formation and multi-host transmission evolve from divergent genetic backgrounds inCampylobacter jejuni
title_sort Enhanced biofilm formation and multi-host transmission evolve from divergent genetic backgrounds inCampylobacter jejuni
author_id_str_mv 4660c0eb7e6bfd796cd749ae713ea558
9384e450ee619395be7459d6bd7a8f6d
author_id_fullname_str_mv 4660c0eb7e6bfd796cd749ae713ea558_***_Ben Pascoe
9384e450ee619395be7459d6bd7a8f6d_***_Guillaume Meric
author Ben Pascoe
Guillaume Meric
author2 Ben Pascoe
Guillaume Méric
Susan Murray
Koji Yahara
Leonardos Mageiros
Ryan Bowen
Nathan H. Jones
Rose E. Jeeves
Hilary M. Lappin-Scott
Hiroshi Asakura
Samuel K. Sheppard
Guillaume Meric
format Journal article
container_title Environmental Microbiology
publishDate 2015
institution Swansea University
doi_str_mv 10.1111/1462-2920.13051
college_str Faculty of Medicine, Health and Life Sciences
hierarchytype
hierarchy_top_id facultyofmedicinehealthandlifesciences
hierarchy_top_title Faculty of Medicine, Health and Life Sciences
hierarchy_parent_id facultyofmedicinehealthandlifesciences
hierarchy_parent_title Faculty of Medicine, Health and Life Sciences
department_str Swansea University Medical School - Medicine{{{_:::_}}}Faculty of Medicine, Health and Life Sciences{{{_:::_}}}Swansea University Medical School - Medicine
url http://onlinelibrary.wiley.com/doi/10.1111/1462-2920.13051/abstract
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active_str 0
description Multicellular biofilms are an ancient bacterial adaptation that can offer a protective environment for survival in hostile habitats. In microaerophilic organisms like Campylobacter, biofilms can play a key role in transmission to humans as the bacteria are exposed to atmospheric oxygen concentrations when leaving the reservoir host gut. The genetic determinants of biofilm formation can differ between species but little is known about how strains of the same species achieve the biofilm phenotype with different genetic backgrounds. We take an integrated genomics approach to combine genome-wide association studies with traditional microbiology techniques to investigate the genetic basis of biofilm formation in 102 Campylobacter jejuni isolates. The study quantified biofilm formation among the isolates and identified hotspots of genetic variation in homologous sequence that corresponded to variation in biofilm phenotypes. In total, 46 genes showed a statistically robust association including those involved in adhesion, motility, nitrosative and oxidative stress. Previously reported and novel genes, containing associated elements, clustered on the genome - including 9 multi-gene transcriptional units. This is consistent with genetic linkage and the horizontal acquisition of biofilm genes in recombining organisms such as C. jejuni. The genes associated with biofilm formation were different in the host generalist ST-21 and ST-45 clonal complexes, which are frequently isolated from multiple host species and clinical samples, but both had enhanced biofilm formation compared to host specialists. This suggests the evolution of enhanced biofilm from different genetic backgrounds and a possible role in colonisation of multiple hosts and transmission to humans.
published_date 2015-09-16T03:22:00Z
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score 11.036531

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