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Generic epitaxial graphene biosensors for ultrasensitive detection of cancer risk biomarker

Zari Tehrani Orcid Logo, G Burwell, M A Mohd Azmi, A Castaing, R Rickman, J Almarashi, Peter Dunstan Orcid Logo, A Miran Beigi, shareen Doak, Owen Guy Orcid Logo

2D Materials, Volume: 1, Issue: 2, Start page: 025004

Swansea University Authors: Zari Tehrani Orcid Logo, Peter Dunstan Orcid Logo, shareen Doak, Owen Guy Orcid Logo

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DOI (Published version): 10.1088/2053-1583/1/2/025004

Abstract

A generic electrochemical method of 'bioreceptor' antibody attachment to phenyl amine functionalized graphitic surfaces is demonstrated. Micro-channels of chemically modified multi-layer epitaxial graphene (MLEG) have been used to provide a repeatable and reliable response to nano-molar (n...

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Published in: 2D Materials
ISSN: 2053-1583
Published: 2014
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URI: https://cronfa.swan.ac.uk/Record/cronfa19735
first_indexed 2014-12-13T02:56:44Z
last_indexed 2018-12-05T04:22:39Z
id cronfa19735
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spelling 2018-12-04T10:43:21.2864836 v2 19735 2014-12-12 Generic epitaxial graphene biosensors for ultrasensitive detection of cancer risk biomarker fd8e614b01086804c80fbafa6fa6aaf5 0000-0002-5069-7921 Zari Tehrani Zari Tehrani true false eada15d4d33fcb3dfddcff43f1323bd6 0000-0002-4337-4307 Peter Dunstan Peter Dunstan true false 8f70286908f67238a527a98cbf66d387 shareen Doak shareen Doak true false c7fa5949b8528e048c5b978005f66794 0000-0002-6449-4033 Owen Guy Owen Guy true false 2014-12-12 EAAS A generic electrochemical method of 'bioreceptor' antibody attachment to phenyl amine functionalized graphitic surfaces is demonstrated. Micro-channels of chemically modified multi-layer epitaxial graphene (MLEG) have been used to provide a repeatable and reliable response to nano-molar (nM) concentrations of the cancer risk (oxidative stress) biomarker 8-hydroxydeoxyguanosine (8-OHdG). X-ray photoelectron spectroscopy, Raman spectroscopy are used to characterize the functionalized MLEG. Confocal fluorescence microscopy using fluorescent-labelled antibodies indicates that the anti-8-OHdG antibody selectively binds to the phenyl amine-functionalized MLEG's channel. Current–voltage measurements on functionalized channels showed repeatable current responses from antibody–biomarker binding events. This technique is scalable, reliable, and capable of providing a rapid, quantitative, label-free assessment of biomarkers at nano-molar (<20 nM) concentrations in analyte solutions. The sensitivity of the sensor device was investigated using varying concentrations of 8-OHdG, with changes in the sensor's channel resistance observed upon exposure to 8-OHdG. Detection of 8-OHdG concentrations as low as 0.1 ng ml−1 (0.35 nM) has been demonstrated. This is five times more sensitive than reported enzyme linked immunosorbent assay tests (0.5 ng ml−1). Journal Article 2D Materials 1 2 025004 2053-1583 19 9 2014 2014-09-19 10.1088/2053-1583/1/2/025004 COLLEGE NANME Engineering and Applied Sciences School COLLEGE CODE EAAS Swansea University 2018-12-04T10:43:21.2864836 2014-12-12T14:48:56.3772260 Zari Tehrani 0000-0002-5069-7921 1 G Burwell 2 M A Mohd Azmi 3 A Castaing 4 R Rickman 5 J Almarashi 6 Peter Dunstan 0000-0002-4337-4307 7 A Miran Beigi 8 shareen Doak 9 Owen Guy 0000-0002-6449-4033 10 0019735-07052015130054.pdf Zari-2014-FINAL__FOR__IOPfor__reviewer__OJ__Zari__V2__pdf.pdf 2015-05-07T13:00:54.2600000 Output 1625804 application/pdf Accepted Manuscript true 2015-05-07T00:00:00.0000000 false
title Generic epitaxial graphene biosensors for ultrasensitive detection of cancer risk biomarker
spellingShingle Generic epitaxial graphene biosensors for ultrasensitive detection of cancer risk biomarker
Zari Tehrani
Peter Dunstan
shareen Doak
Owen Guy
title_short Generic epitaxial graphene biosensors for ultrasensitive detection of cancer risk biomarker
title_full Generic epitaxial graphene biosensors for ultrasensitive detection of cancer risk biomarker
title_fullStr Generic epitaxial graphene biosensors for ultrasensitive detection of cancer risk biomarker
title_full_unstemmed Generic epitaxial graphene biosensors for ultrasensitive detection of cancer risk biomarker
title_sort Generic epitaxial graphene biosensors for ultrasensitive detection of cancer risk biomarker
author_id_str_mv fd8e614b01086804c80fbafa6fa6aaf5
eada15d4d33fcb3dfddcff43f1323bd6
8f70286908f67238a527a98cbf66d387
c7fa5949b8528e048c5b978005f66794
author_id_fullname_str_mv fd8e614b01086804c80fbafa6fa6aaf5_***_Zari Tehrani
eada15d4d33fcb3dfddcff43f1323bd6_***_Peter Dunstan
8f70286908f67238a527a98cbf66d387_***_shareen Doak
c7fa5949b8528e048c5b978005f66794_***_Owen Guy
author Zari Tehrani
Peter Dunstan
shareen Doak
Owen Guy
author2 Zari Tehrani
G Burwell
M A Mohd Azmi
A Castaing
R Rickman
J Almarashi
Peter Dunstan
A Miran Beigi
shareen Doak
Owen Guy
format Journal article
container_title 2D Materials
container_volume 1
container_issue 2
container_start_page 025004
publishDate 2014
institution Swansea University
issn 2053-1583
doi_str_mv 10.1088/2053-1583/1/2/025004
document_store_str 1
active_str 0
description A generic electrochemical method of 'bioreceptor' antibody attachment to phenyl amine functionalized graphitic surfaces is demonstrated. Micro-channels of chemically modified multi-layer epitaxial graphene (MLEG) have been used to provide a repeatable and reliable response to nano-molar (nM) concentrations of the cancer risk (oxidative stress) biomarker 8-hydroxydeoxyguanosine (8-OHdG). X-ray photoelectron spectroscopy, Raman spectroscopy are used to characterize the functionalized MLEG. Confocal fluorescence microscopy using fluorescent-labelled antibodies indicates that the anti-8-OHdG antibody selectively binds to the phenyl amine-functionalized MLEG's channel. Current–voltage measurements on functionalized channels showed repeatable current responses from antibody–biomarker binding events. This technique is scalable, reliable, and capable of providing a rapid, quantitative, label-free assessment of biomarkers at nano-molar (<20 nM) concentrations in analyte solutions. The sensitivity of the sensor device was investigated using varying concentrations of 8-OHdG, with changes in the sensor's channel resistance observed upon exposure to 8-OHdG. Detection of 8-OHdG concentrations as low as 0.1 ng ml−1 (0.35 nM) has been demonstrated. This is five times more sensitive than reported enzyme linked immunosorbent assay tests (0.5 ng ml−1).
published_date 2014-09-19T03:36:01Z
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