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Real-time detection of hepatitis B surface antigen using a hybrid graphene-gold nanoparticle biosensor / Ffion Walters; S Rozhko; D Buckley; Ehsaneh Daghigh Ahmadi; Muhammad Ali; Zari Tehrani; Jacob Mitchell; Gregory Burwell; Y Liu; O Kazakova; Owen Guy

2D Materials, Volume: 7, Issue: 2, Start page: 024009

Swansea University Authors: Ffion, Walters, Ehsaneh, Daghigh Ahmadi, Muhammad, Ali, Zari, Tehrani, Jacob, Mitchell, Gregory, Burwell, Owen, Guy

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Abstract

A hybrid biosensor based on a graphene resistor functionalized with self-assembled Graphene-AuNPs (Gold Nanoparticles) is demonstrated for the real-time detection of hepatitis B surface antigen (HBsAg). The hybrid biosensor consists of a ssDNA sequence attached to a graphene resistor device via π–π...

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Published in: 2D Materials
ISSN: 2053-1583
Published: IOP Publishing 2020
Online Access: Check full text

URI: https://cronfa.swan.ac.uk/Record/cronfa53518
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Abstract: A hybrid biosensor based on a graphene resistor functionalized with self-assembled Graphene-AuNPs (Gold Nanoparticles) is demonstrated for the real-time detection of hepatitis B surface antigen (HBsAg). The hybrid biosensor consists of a ssDNA sequence attached to a graphene resistor device via π–π stacking interactions in combination with a ssDNA functionalized AuNP. The ssDNA has complementary sequences which through hybridization, yield the graphene-AuNP hybrid biosensor. Real-time 2-point resistance measurements, performed using varying concentrations of HBsAg, show a linear dependence of resistance change against the logarithm of HBsAg concentration (log[HBsAg]). A limit of detection of 50 pg ml−1 was observed. Moreover, the hybrid biosensor platform has potential to be applied to any biomarker of interest.
Keywords: graphene, hepatitis, gold nanoparticle, biosensor, ssDNA, detection, AFM-IR
College: College of Science
Issue: 2
Start Page: 024009