Real-time detection of hepatitis B surface antigen using a hybrid graphene-gold nanoparticle biosensor

Walters, Ffion; Rozhko, S; Buckley, D; Ahmadi, Ehsaneh Daghigh; Ali, Muhammad; Tehrani, Zari; Mitchell, Jacob; Burwell, Gregory; Liu, Y; Kazakova, O; Guy, Owen

doi:10.1088/2053-1583/ab734c

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Real-time detection of hepatitis B surface antigen using a hybrid graphene-gold nanoparticle biosensor

Ffion Walters

, S Rozhko, D Buckley, Ehsaneh Daghigh Ahmadi, Muhammad Ali, Zari Tehrani

, Jacob Mitchell, Gregory Burwell

, Y Liu, O Kazakova, Owen Guy

2D Materials, Volume: 7, Issue: 2, Start page: 024009

Swansea University Authors: Ffion Walters , Ehsaneh Daghigh Ahmadi, Muhammad Ali, Zari Tehrani , Jacob Mitchell, Gregory Burwell , Owen Guy

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DOI (Published version): 10.1088/2053-1583/ab734c

Abstract

A hybrid biosensor based on a graphene resistor functionalized with self-assembled Graphene-AuNPs (Gold Nanoparticles) is demonstrated for the real-time detection of hepatitis B surface antigen (HBsAg). The hybrid biosensor consists of a ssDNA sequence attached to a graphene resistor device via π–π...

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Published in:	2D Materials
ISSN:	2053-1583
Published:	IOP Publishing 2020
Online Access:	Check full text
URI:	https://cronfa.swan.ac.uk/Record/cronfa53518
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first_indexed	2020-02-13T19:46:06Z
last_indexed	2021-09-25T03:14:24Z
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The hybrid biosensor consists of a ssDNA sequence attached to a graphene resistor device via π–π stacking interactions in combination with a ssDNA functionalized AuNP. The ssDNA has complementary sequences which through hybridization, yield the graphene-AuNP hybrid biosensor. Real-time 2-point resistance measurements, performed using varying concentrations of HBsAg, show a linear dependence of resistance change against the logarithm of HBsAg concentration (log[HBsAg]). A limit of detection of 50 pg ml−1 was observed. 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spelling	2021-09-24T15:54:41.0639908 v2 53518 2020-02-13 Real-time detection of hepatitis B surface antigen using a hybrid graphene-gold nanoparticle biosensor 7808dfe3fba8a2989fd755a8a9e08d0c 0000-0003-1732-6886 Ffion Walters Ffion Walters true false 974f6a7393c1f088d58aeeea07d80363 Ehsaneh Daghigh Ahmadi Ehsaneh Daghigh Ahmadi true false 103ad6374ddc3a36f8d0609a8f471535 Muhammad Ali Muhammad Ali true false fd8e614b01086804c80fbafa6fa6aaf5 0000-0002-5069-7921 Zari Tehrani Zari Tehrani true false 522a9b94c350f5977584e0fd942facdc Jacob Mitchell Jacob Mitchell true false 49890fbfbe127d4ae94bc10dc2b24199 0000-0002-2534-9626 Gregory Burwell Gregory Burwell true false c7fa5949b8528e048c5b978005f66794 0000-0002-6449-4033 Owen Guy Owen Guy true false 2020-02-13 FGMHL A hybrid biosensor based on a graphene resistor functionalized with self-assembled Graphene-AuNPs (Gold Nanoparticles) is demonstrated for the real-time detection of hepatitis B surface antigen (HBsAg). The hybrid biosensor consists of a ssDNA sequence attached to a graphene resistor device via π–π stacking interactions in combination with a ssDNA functionalized AuNP. The ssDNA has complementary sequences which through hybridization, yield the graphene-AuNP hybrid biosensor. Real-time 2-point resistance measurements, performed using varying concentrations of HBsAg, show a linear dependence of resistance change against the logarithm of HBsAg concentration (log[HBsAg]). A limit of detection of 50 pg ml−1 was observed. Moreover, the hybrid biosensor platform has potential to be applied to any biomarker of interest. Journal Article 2D Materials 7 2 024009 IOP Publishing 2053-1583 graphene, hepatitis, gold nanoparticle, biosensor, ssDNA, detection, AFM-IR 5 3 2020 2020-03-05 10.1088/2053-1583/ab734c COLLEGE NANME Medicine, Health and Life Science - Faculty COLLEGE CODE FGMHL Swansea University 2021-09-24T15:54:41.0639908 2020-02-13T12:58:11.8325260 Faculty of Science and Engineering School of Engineering and Applied Sciences - Chemistry Ffion Walters 0000-0003-1732-6886 1 S Rozhko 2 D Buckley 3 Ehsaneh Daghigh Ahmadi 4 Muhammad Ali 5 Zari Tehrani 0000-0002-5069-7921 6 Jacob Mitchell 7 Gregory Burwell 0000-0002-2534-9626 8 Y Liu 9 O Kazakova 10 Owen Guy 0000-0002-6449-4033 11 53518__18893__e70fb28289b54df589682f8d588333db.pdf 53518.pdf 2020-12-15T15:22:58.0136931 Output 2289877 application/pdf Version of Record true © 2020 Author(s). All article content, except where otherwise noted, is licensed under a Creative Commons Attribution 4.0 (CC BY) License true eng http://creativecommons.org/licenses/by/4.0
title	Real-time detection of hepatitis B surface antigen using a hybrid graphene-gold nanoparticle biosensor
spellingShingle	Real-time detection of hepatitis B surface antigen using a hybrid graphene-gold nanoparticle biosensor Ffion Walters Ehsaneh Daghigh Ahmadi Muhammad Ali Zari Tehrani Jacob Mitchell Gregory Burwell Owen Guy
title_short	Real-time detection of hepatitis B surface antigen using a hybrid graphene-gold nanoparticle biosensor
title_full	Real-time detection of hepatitis B surface antigen using a hybrid graphene-gold nanoparticle biosensor
title_fullStr	Real-time detection of hepatitis B surface antigen using a hybrid graphene-gold nanoparticle biosensor
title_full_unstemmed	Real-time detection of hepatitis B surface antigen using a hybrid graphene-gold nanoparticle biosensor
title_sort	Real-time detection of hepatitis B surface antigen using a hybrid graphene-gold nanoparticle biosensor
author_id_str_mv	7808dfe3fba8a2989fd755a8a9e08d0c 974f6a7393c1f088d58aeeea07d80363 103ad6374ddc3a36f8d0609a8f471535 fd8e614b01086804c80fbafa6fa6aaf5 522a9b94c350f5977584e0fd942facdc 49890fbfbe127d4ae94bc10dc2b24199 c7fa5949b8528e048c5b978005f66794
author_id_fullname_str_mv	7808dfe3fba8a2989fd755a8a9e08d0c_*_Ffion Walters 974f6a7393c1f088d58aeeea07d80363__Ehsaneh Daghigh Ahmadi 103ad6374ddc3a36f8d0609a8f471535__Muhammad Ali fd8e614b01086804c80fbafa6fa6aaf5__Zari Tehrani 522a9b94c350f5977584e0fd942facdc__Jacob Mitchell 49890fbfbe127d4ae94bc10dc2b24199__Gregory Burwell c7fa5949b8528e048c5b978005f66794_**_Owen Guy
author	Ffion Walters Ehsaneh Daghigh Ahmadi Muhammad Ali Zari Tehrani Jacob Mitchell Gregory Burwell Owen Guy
author2	Ffion Walters S Rozhko D Buckley Ehsaneh Daghigh Ahmadi Muhammad Ali Zari Tehrani Jacob Mitchell Gregory Burwell Y Liu O Kazakova Owen Guy
format	Journal article
container_title	2D Materials
container_volume	7
container_issue	2
container_start_page	024009
publishDate	2020
institution	Swansea University
issn	2053-1583
doi_str_mv	10.1088/2053-1583/ab734c
publisher	IOP Publishing
college_str	Faculty of Science and Engineering
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hierarchy_top_id	facultyofscienceandengineering
hierarchy_top_title	Faculty of Science and Engineering
hierarchy_parent_id	facultyofscienceandengineering
hierarchy_parent_title	Faculty of Science and Engineering
department_str	School of Engineering and Applied Sciences - Chemistry{{{_:::_}}}Faculty of Science and Engineering{{{_:::_}}}School of Engineering and Applied Sciences - Chemistry
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description	A hybrid biosensor based on a graphene resistor functionalized with self-assembled Graphene-AuNPs (Gold Nanoparticles) is demonstrated for the real-time detection of hepatitis B surface antigen (HBsAg). The hybrid biosensor consists of a ssDNA sequence attached to a graphene resistor device via π–π stacking interactions in combination with a ssDNA functionalized AuNP. The ssDNA has complementary sequences which through hybridization, yield the graphene-AuNP hybrid biosensor. Real-time 2-point resistance measurements, performed using varying concentrations of HBsAg, show a linear dependence of resistance change against the logarithm of HBsAg concentration (log[HBsAg]). A limit of detection of 50 pg ml−1 was observed. Moreover, the hybrid biosensor platform has potential to be applied to any biomarker of interest.
published_date	2020-03-05T04:06:29Z
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Real-time detection of hepatitis B surface antigen using a hybrid graphene-gold nanoparticle biosensor

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