No Cover Image

Journal article 310 views 33 downloads

Real-time detection of hepatitis B surface antigen using a hybrid graphene-gold nanoparticle biosensor

Ffion Walters, S Rozhko, D Buckley, Ehsaneh Daghigh Ahmadi, Muhammad Ali, Zari Tehrani Orcid Logo, Jacob Mitchell Orcid Logo, Gregory Burwell Orcid Logo, Y Liu, O Kazakova, Owen Guy Orcid Logo

2D Materials, Volume: 7, Issue: 2, Start page: 024009

Swansea University Authors: Ffion Walters, Ehsaneh Daghigh Ahmadi, Muhammad Ali, Zari Tehrani Orcid Logo, Jacob Mitchell Orcid Logo, Gregory Burwell Orcid Logo, Owen Guy Orcid Logo

  • 53518.pdf

    PDF | Version of Record

    © 2020 Author(s). All article content, except where otherwise noted, is licensed under a Creative Commons Attribution 4.0 (CC BY) License

    Download (2.18MB)

Abstract

A hybrid biosensor based on a graphene resistor functionalized with self-assembled Graphene-AuNPs (Gold Nanoparticles) is demonstrated for the real-time detection of hepatitis B surface antigen (HBsAg). The hybrid biosensor consists of a ssDNA sequence attached to a graphene resistor device via π–π...

Full description

Published in: 2D Materials
ISSN: 2053-1583
Published: IOP Publishing 2020
Online Access: Check full text

URI: https://cronfa.swan.ac.uk/Record/cronfa53518
Tags: Add Tag
No Tags, Be the first to tag this record!
first_indexed 2020-02-13T19:46:06Z
last_indexed 2021-09-25T03:14:24Z
id cronfa53518
recordtype SURis
fullrecord <?xml version="1.0"?><rfc1807><datestamp>2021-09-24T15:54:41.0639908</datestamp><bib-version>v2</bib-version><id>53518</id><entry>2020-02-13</entry><title>Real-time detection of hepatitis B surface antigen using a hybrid graphene-gold nanoparticle biosensor</title><swanseaauthors><author><sid>7808dfe3fba8a2989fd755a8a9e08d0c</sid><firstname>Ffion</firstname><surname>Walters</surname><name>Ffion Walters</name><active>true</active><ethesisStudent>false</ethesisStudent></author><author><sid>974f6a7393c1f088d58aeeea07d80363</sid><firstname>Ehsaneh</firstname><surname>Daghigh Ahmadi</surname><name>Ehsaneh Daghigh Ahmadi</name><active>true</active><ethesisStudent>false</ethesisStudent></author><author><sid>103ad6374ddc3a36f8d0609a8f471535</sid><firstname>Muhammad</firstname><surname>Ali</surname><name>Muhammad Ali</name><active>true</active><ethesisStudent>false</ethesisStudent></author><author><sid>fd8e614b01086804c80fbafa6fa6aaf5</sid><ORCID>0000-0002-5069-7921</ORCID><firstname>Zari</firstname><surname>Tehrani</surname><name>Zari Tehrani</name><active>true</active><ethesisStudent>false</ethesisStudent></author><author><sid>522a9b94c350f5977584e0fd942facdc</sid><ORCID>0000-0003-1377-725X</ORCID><firstname>Jacob</firstname><surname>Mitchell</surname><name>Jacob Mitchell</name><active>true</active><ethesisStudent>false</ethesisStudent></author><author><sid>49890fbfbe127d4ae94bc10dc2b24199</sid><ORCID>0000-0002-2534-9626</ORCID><firstname>Gregory</firstname><surname>Burwell</surname><name>Gregory Burwell</name><active>true</active><ethesisStudent>false</ethesisStudent></author><author><sid>c7fa5949b8528e048c5b978005f66794</sid><ORCID>0000-0002-6449-4033</ORCID><firstname>Owen</firstname><surname>Guy</surname><name>Owen Guy</name><active>true</active><ethesisStudent>false</ethesisStudent></author></swanseaauthors><date>2020-02-13</date><deptcode>BMS</deptcode><abstract>A hybrid biosensor based on a graphene resistor functionalized with self-assembled Graphene-AuNPs (Gold Nanoparticles) is demonstrated for the real-time detection of hepatitis B surface antigen (HBsAg). The hybrid biosensor consists of a ssDNA sequence attached to a graphene resistor device via &#x3C0;&#x2013;&#x3C0; stacking interactions in combination with a ssDNA functionalized AuNP. The ssDNA has complementary sequences which through hybridization, yield the graphene-AuNP hybrid biosensor. Real-time 2-point resistance measurements, performed using varying concentrations of HBsAg, show a linear dependence of resistance change against the logarithm of HBsAg concentration (log[HBsAg]). A limit of detection of 50 pg ml&#x2212;1 was observed. Moreover, the hybrid biosensor platform has potential to be applied to any biomarker of interest.</abstract><type>Journal Article</type><journal>2D Materials</journal><volume>7</volume><journalNumber>2</journalNumber><paginationStart>024009</paginationStart><paginationEnd/><publisher>IOP Publishing</publisher><placeOfPublication/><isbnPrint/><isbnElectronic/><issnPrint/><issnElectronic>2053-1583</issnElectronic><keywords>graphene, hepatitis, gold nanoparticle, biosensor, ssDNA, detection, AFM-IR</keywords><publishedDay>5</publishedDay><publishedMonth>3</publishedMonth><publishedYear>2020</publishedYear><publishedDate>2020-03-05</publishedDate><doi>10.1088/2053-1583/ab734c</doi><url/><notes/><college>COLLEGE NANME</college><department>Biomedical Sciences</department><CollegeCode>COLLEGE CODE</CollegeCode><DepartmentCode>BMS</DepartmentCode><institution>Swansea University</institution><apcterm/><lastEdited>2021-09-24T15:54:41.0639908</lastEdited><Created>2020-02-13T12:58:11.8325260</Created><path><level id="1">College of Science</level><level id="2">Chemistry</level></path><authors><author><firstname>Ffion</firstname><surname>Walters</surname><order>1</order></author><author><firstname>S</firstname><surname>Rozhko</surname><order>2</order></author><author><firstname>D</firstname><surname>Buckley</surname><order>3</order></author><author><firstname>Ehsaneh</firstname><surname>Daghigh Ahmadi</surname><order>4</order></author><author><firstname>Muhammad</firstname><surname>Ali</surname><order>5</order></author><author><firstname>Zari</firstname><surname>Tehrani</surname><orcid>0000-0002-5069-7921</orcid><order>6</order></author><author><firstname>Jacob</firstname><surname>Mitchell</surname><orcid>0000-0003-1377-725X</orcid><order>7</order></author><author><firstname>Gregory</firstname><surname>Burwell</surname><orcid>0000-0002-2534-9626</orcid><order>8</order></author><author><firstname>Y</firstname><surname>Liu</surname><order>9</order></author><author><firstname>O</firstname><surname>Kazakova</surname><order>10</order></author><author><firstname>Owen</firstname><surname>Guy</surname><orcid>0000-0002-6449-4033</orcid><order>11</order></author></authors><documents><document><filename>53518__18893__e70fb28289b54df589682f8d588333db.pdf</filename><originalFilename>53518.pdf</originalFilename><uploaded>2020-12-15T15:22:58.0136931</uploaded><type>Output</type><contentLength>2289877</contentLength><contentType>application/pdf</contentType><version>Version of Record</version><cronfaStatus>true</cronfaStatus><documentNotes>&#xA9; 2020 Author(s). All article content, except where otherwise noted, is licensed under a Creative Commons Attribution 4.0 (CC BY) License</documentNotes><copyrightCorrect>true</copyrightCorrect><language>eng</language><licence>http://creativecommons.org/licenses/by/4.0</licence></document></documents><OutputDurs/></rfc1807>
spelling 2021-09-24T15:54:41.0639908 v2 53518 2020-02-13 Real-time detection of hepatitis B surface antigen using a hybrid graphene-gold nanoparticle biosensor 7808dfe3fba8a2989fd755a8a9e08d0c Ffion Walters Ffion Walters true false 974f6a7393c1f088d58aeeea07d80363 Ehsaneh Daghigh Ahmadi Ehsaneh Daghigh Ahmadi true false 103ad6374ddc3a36f8d0609a8f471535 Muhammad Ali Muhammad Ali true false fd8e614b01086804c80fbafa6fa6aaf5 0000-0002-5069-7921 Zari Tehrani Zari Tehrani true false 522a9b94c350f5977584e0fd942facdc 0000-0003-1377-725X Jacob Mitchell Jacob Mitchell true false 49890fbfbe127d4ae94bc10dc2b24199 0000-0002-2534-9626 Gregory Burwell Gregory Burwell true false c7fa5949b8528e048c5b978005f66794 0000-0002-6449-4033 Owen Guy Owen Guy true false 2020-02-13 BMS A hybrid biosensor based on a graphene resistor functionalized with self-assembled Graphene-AuNPs (Gold Nanoparticles) is demonstrated for the real-time detection of hepatitis B surface antigen (HBsAg). The hybrid biosensor consists of a ssDNA sequence attached to a graphene resistor device via π–π stacking interactions in combination with a ssDNA functionalized AuNP. The ssDNA has complementary sequences which through hybridization, yield the graphene-AuNP hybrid biosensor. Real-time 2-point resistance measurements, performed using varying concentrations of HBsAg, show a linear dependence of resistance change against the logarithm of HBsAg concentration (log[HBsAg]). A limit of detection of 50 pg ml−1 was observed. Moreover, the hybrid biosensor platform has potential to be applied to any biomarker of interest. Journal Article 2D Materials 7 2 024009 IOP Publishing 2053-1583 graphene, hepatitis, gold nanoparticle, biosensor, ssDNA, detection, AFM-IR 5 3 2020 2020-03-05 10.1088/2053-1583/ab734c COLLEGE NANME Biomedical Sciences COLLEGE CODE BMS Swansea University 2021-09-24T15:54:41.0639908 2020-02-13T12:58:11.8325260 College of Science Chemistry Ffion Walters 1 S Rozhko 2 D Buckley 3 Ehsaneh Daghigh Ahmadi 4 Muhammad Ali 5 Zari Tehrani 0000-0002-5069-7921 6 Jacob Mitchell 0000-0003-1377-725X 7 Gregory Burwell 0000-0002-2534-9626 8 Y Liu 9 O Kazakova 10 Owen Guy 0000-0002-6449-4033 11 53518__18893__e70fb28289b54df589682f8d588333db.pdf 53518.pdf 2020-12-15T15:22:58.0136931 Output 2289877 application/pdf Version of Record true © 2020 Author(s). All article content, except where otherwise noted, is licensed under a Creative Commons Attribution 4.0 (CC BY) License true eng http://creativecommons.org/licenses/by/4.0
title Real-time detection of hepatitis B surface antigen using a hybrid graphene-gold nanoparticle biosensor
spellingShingle Real-time detection of hepatitis B surface antigen using a hybrid graphene-gold nanoparticle biosensor
Ffion Walters
Ehsaneh Daghigh Ahmadi
Muhammad Ali
Zari Tehrani
Jacob Mitchell
Gregory Burwell
Owen Guy
title_short Real-time detection of hepatitis B surface antigen using a hybrid graphene-gold nanoparticle biosensor
title_full Real-time detection of hepatitis B surface antigen using a hybrid graphene-gold nanoparticle biosensor
title_fullStr Real-time detection of hepatitis B surface antigen using a hybrid graphene-gold nanoparticle biosensor
title_full_unstemmed Real-time detection of hepatitis B surface antigen using a hybrid graphene-gold nanoparticle biosensor
title_sort Real-time detection of hepatitis B surface antigen using a hybrid graphene-gold nanoparticle biosensor
author_id_str_mv 7808dfe3fba8a2989fd755a8a9e08d0c
974f6a7393c1f088d58aeeea07d80363
103ad6374ddc3a36f8d0609a8f471535
fd8e614b01086804c80fbafa6fa6aaf5
522a9b94c350f5977584e0fd942facdc
49890fbfbe127d4ae94bc10dc2b24199
c7fa5949b8528e048c5b978005f66794
author_id_fullname_str_mv 7808dfe3fba8a2989fd755a8a9e08d0c_***_Ffion Walters
974f6a7393c1f088d58aeeea07d80363_***_Ehsaneh Daghigh Ahmadi
103ad6374ddc3a36f8d0609a8f471535_***_Muhammad Ali
fd8e614b01086804c80fbafa6fa6aaf5_***_Zari Tehrani
522a9b94c350f5977584e0fd942facdc_***_Jacob Mitchell
49890fbfbe127d4ae94bc10dc2b24199_***_Gregory Burwell
c7fa5949b8528e048c5b978005f66794_***_Owen Guy
author Ffion Walters
Ehsaneh Daghigh Ahmadi
Muhammad Ali
Zari Tehrani
Jacob Mitchell
Gregory Burwell
Owen Guy
author2 Ffion Walters
S Rozhko
D Buckley
Ehsaneh Daghigh Ahmadi
Muhammad Ali
Zari Tehrani
Jacob Mitchell
Gregory Burwell
Y Liu
O Kazakova
Owen Guy
format Journal article
container_title 2D Materials
container_volume 7
container_issue 2
container_start_page 024009
publishDate 2020
institution Swansea University
issn 2053-1583
doi_str_mv 10.1088/2053-1583/ab734c
publisher IOP Publishing
college_str College of Science
hierarchytype
hierarchy_top_id collegeofscience
hierarchy_top_title College of Science
hierarchy_parent_id collegeofscience
hierarchy_parent_title College of Science
department_str Chemistry{{{_:::_}}}College of Science{{{_:::_}}}Chemistry
document_store_str 1
active_str 0
description A hybrid biosensor based on a graphene resistor functionalized with self-assembled Graphene-AuNPs (Gold Nanoparticles) is demonstrated for the real-time detection of hepatitis B surface antigen (HBsAg). The hybrid biosensor consists of a ssDNA sequence attached to a graphene resistor device via π–π stacking interactions in combination with a ssDNA functionalized AuNP. The ssDNA has complementary sequences which through hybridization, yield the graphene-AuNP hybrid biosensor. Real-time 2-point resistance measurements, performed using varying concentrations of HBsAg, show a linear dependence of resistance change against the logarithm of HBsAg concentration (log[HBsAg]). A limit of detection of 50 pg ml−1 was observed. Moreover, the hybrid biosensor platform has potential to be applied to any biomarker of interest.
published_date 2020-03-05T04:24:22Z
_version_ 1733042228625932288
score 10.8721