Journal article 614 views 87 downloads
A Facile Method for the Non-Covalent Amine Functionalization of Carbon-Based Surfaces for Use in Biosensor Development
,
Muhammad Ali,
Gregory Burwell ,
Sergiy Rozhko,
Zari Tehrani ,
Ehsaneh Daghigh Ahmadi,
Jonathan Evans,
Hina Abbasi,
Ryan Bigham,
Jacob Mitchell,
Olga Kazakova,
Anitha Devadoss ,
Owen Guy
Nanomaterials, Volume: 10, Issue: 9
Swansea University Authors:
Ffion Walters , Muhammad Ali, Gregory Burwell , Zari Tehrani , Ehsaneh Daghigh Ahmadi, Jonathan Evans, Hina Abbasi, Ryan Bigham, Jacob Mitchell, Anitha Devadoss , Owen Guy
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DOI (Published version): 10.3390/nano10091808
Abstract
Affinity biosensors based on graphene field-effect transistor (GFET) or resistor designs require the utilization of graphene’s exceptional electrical properties. Therefore, it is critical when designing these sensors, that the electrical properties of graphene are maintained throughout the functiona...
| Published in: | Nanomaterials |
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| ISSN: | 2079-4991 |
| Published: |
MDPI AG
2020
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| Online Access: |
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| URI: | https://cronfa.swan.ac.uk/Record/cronfa55139 |
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| Abstract: |
Affinity biosensors based on graphene field-effect transistor (GFET) or resistor designs require the utilization of graphene’s exceptional electrical properties. Therefore, it is critical when designing these sensors, that the electrical properties of graphene are maintained throughout the functionalization process. To that end, non-covalent functionalization may be preferred over covalent modification. Drop-cast 1,5-diaminonaphthalene (DAN) was investigated as a quick and simple method for the non-covalent amine functionalization of carbon-based surfaces such as graphene, for use in biosensor development. In this work, multiple graphene surfaces were functionalized with DAN via a drop-cast method, leading to amine moieties, available for subsequent attachment to receptor molecules. Successful modification of graphene with DAN via a drop-cast method was confirmed using X-ray photoelectron spectroscopy (XPS), Raman spectroscopy and real-time resistance measurements. Successful attachment of receptor molecules also confirmed using the aforementioned techniques. Furthermore, an investigation into the effect of sequential wash steps which are required in biosensor manufacture, on the presence of the DAN layer, confirmed that the functional layer was not removed, even after multiple solvent exposures. Drop-cast DAN is thus, a viable fast and robust method for the amine functionalization of graphene surfaces for use in biosensor development. |
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| Keywords: |
graphene; non-covalent; biosensor; real-time; sensor; nanocomposite; π-π stacking; drop-cast; carbon-surfaces; resistor; GFET |
| College: |
Faculty of Science and Engineering |
| Funders: |
UKRI, EP/M006301/1 |
| Issue: |
9 |