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Electrochemical Biofunctionalization of Highly Oriented Pyrolytic Graphite for Immunosensor Applications
e-Journal of Surface Science and Nanotechnology, Volume: 14, Pages: 193 - 197
Swansea University Authors: Owen Guy , Zari Tehrani
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DOI (Published version): 10.1380/ejssnt.2016.193
Abstract
The present research demonstrates a procedure for surface modification of Highly Oriented Pyrolytic Graphite (HOPG) electrodes intended for use as immunosensors. The HOPG surface is linked to the molecule 8-hydroxydeoxyguanosine (8-OHdG), an oxidative stress biomarker for DNA damage, though the anil...
Published in: | e-Journal of Surface Science and Nanotechnology |
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ISSN: | 1348-0391 |
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2016
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URI: | https://cronfa.swan.ac.uk/Record/cronfa31387 |
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2016-12-08T10:48:36.2754545 v2 31387 2016-12-08 Electrochemical Biofunctionalization of Highly Oriented Pyrolytic Graphite for Immunosensor Applications c7fa5949b8528e048c5b978005f66794 0000-0002-6449-4033 Owen Guy Owen Guy true false fd8e614b01086804c80fbafa6fa6aaf5 0000-0002-5069-7921 Zari Tehrani Zari Tehrani true false 2016-12-08 EAAS The present research demonstrates a procedure for surface modification of Highly Oriented Pyrolytic Graphite (HOPG) electrodes intended for use as immunosensors. The HOPG surface is linked to the molecule 8-hydroxydeoxyguanosine (8-OHdG), an oxidative stress biomarker for DNA damage, though the aniline mediator covalently bonded to electrode and biomarker. An electrochemical procedure to graft the mediator is described and the presence of biomarker at surface is demonstrated by using a fluorescence-labeled immune-reagent. An electrochemical functionalization process has been employed for attachment of functional aminie (NH2) linking groups to graphitic surfaces, which consists of two stages: (i) a reaction with a diazonium salt to covalently bond nitrobenzene groups to the surface and (ii) electrochemical reduction of the nitro group (–NO2) to an amine group (–NH2). The shape of the CV curve indicates that the redox reactions are taking place at the HOPG electrode surface. The amine group can subsequently be used to covalently link to an antibody biorecptor. The presence of 8-OHdG, indicative of DNA damage, has been linked to increased cancer risk. Detection of this oxidative stress biomarker is an important tool for the early diagnosis of disease. Journal Article e-Journal of Surface Science and Nanotechnology 14 0 193 197 1348-0391 31 12 2016 2016-12-31 10.1380/ejssnt.2016.193 COLLEGE NANME Engineering and Applied Sciences School COLLEGE CODE EAAS Swansea University 2016-12-08T10:48:36.2754545 2016-12-08T10:45:23.9877489 Faculty of Science and Engineering School of Engineering and Applied Sciences - Chemistry Owen Guy 0000-0002-6449-4033 1 Z. Tehrani 2 D. J. Thomas 3 O. J. Guy 4 Zari Tehrani 0000-0002-5069-7921 5 0031387-08122016104824.pdf tehrani2016(2).pdf 2016-12-08T10:48:24.5900000 Output 2537263 application/pdf Version of Record true 2016-12-08T00:00:00.0000000 false |
title |
Electrochemical Biofunctionalization of Highly Oriented Pyrolytic Graphite for Immunosensor Applications |
spellingShingle |
Electrochemical Biofunctionalization of Highly Oriented Pyrolytic Graphite for Immunosensor Applications Owen Guy Zari Tehrani |
title_short |
Electrochemical Biofunctionalization of Highly Oriented Pyrolytic Graphite for Immunosensor Applications |
title_full |
Electrochemical Biofunctionalization of Highly Oriented Pyrolytic Graphite for Immunosensor Applications |
title_fullStr |
Electrochemical Biofunctionalization of Highly Oriented Pyrolytic Graphite for Immunosensor Applications |
title_full_unstemmed |
Electrochemical Biofunctionalization of Highly Oriented Pyrolytic Graphite for Immunosensor Applications |
title_sort |
Electrochemical Biofunctionalization of Highly Oriented Pyrolytic Graphite for Immunosensor Applications |
author_id_str_mv |
c7fa5949b8528e048c5b978005f66794 fd8e614b01086804c80fbafa6fa6aaf5 |
author_id_fullname_str_mv |
c7fa5949b8528e048c5b978005f66794_***_Owen Guy fd8e614b01086804c80fbafa6fa6aaf5_***_Zari Tehrani |
author |
Owen Guy Zari Tehrani |
author2 |
Owen Guy Z. Tehrani D. J. Thomas O. J. Guy Zari Tehrani |
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Journal article |
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e-Journal of Surface Science and Nanotechnology |
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14 |
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193 |
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2016 |
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Swansea University |
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1348-0391 |
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10.1380/ejssnt.2016.193 |
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Faculty of Science and Engineering |
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School of Engineering and Applied Sciences - Chemistry{{{_:::_}}}Faculty of Science and Engineering{{{_:::_}}}School of Engineering and Applied Sciences - Chemistry |
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description |
The present research demonstrates a procedure for surface modification of Highly Oriented Pyrolytic Graphite (HOPG) electrodes intended for use as immunosensors. The HOPG surface is linked to the molecule 8-hydroxydeoxyguanosine (8-OHdG), an oxidative stress biomarker for DNA damage, though the aniline mediator covalently bonded to electrode and biomarker. An electrochemical procedure to graft the mediator is described and the presence of biomarker at surface is demonstrated by using a fluorescence-labeled immune-reagent. An electrochemical functionalization process has been employed for attachment of functional aminie (NH2) linking groups to graphitic surfaces, which consists of two stages: (i) a reaction with a diazonium salt to covalently bond nitrobenzene groups to the surface and (ii) electrochemical reduction of the nitro group (–NO2) to an amine group (–NH2). The shape of the CV curve indicates that the redox reactions are taking place at the HOPG electrode surface. The amine group can subsequently be used to covalently link to an antibody biorecptor. The presence of 8-OHdG, indicative of DNA damage, has been linked to increased cancer risk. Detection of this oxidative stress biomarker is an important tool for the early diagnosis of disease. |
published_date |
2016-12-31T07:04:46Z |
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11.085372 |